| Project/Area Number |
20790344
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Fukuoka University |
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Principal Investigator |
ISHII Kazunari Fukuoka University, 医学部, 講師 (70380954)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 微生物 / 免疫学 / 感染症 / ワクチン / T細胞 |
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| Research Abstract |
Chlamydia trachomatis is an obligate intracellular pathogen that causes a variety of disease, including trachoma, pelvic inflammatory diseases, infertility and pneumonia. A vaccine is likely the most effective strategy for controlling human chlamydial infections. Ubiquitin-proteasome pathway is indispensable in inducing MHC class I-restricted CD8^+T cells. In the present study, we applied this strategy for vaccination against C.trachomatis. We constructed a DNA vaccine encoding chlamydia antigen fused with an ubiquitin at its N-terminus for inducing MHC class I-restricted CD8^+ T cells. Mice immunized with the chimeric DNA developed potent protective immunity against Ctrachomatis mediated by antigen-specific CD8^+T cells. The enhanced immunity was dependent on the ubiquitination of antigen that enabled it to be efficiently processed by the proteasome pathway resulting in efficient induction of specific CD8^+ T cells.
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