Biosynthetic and functional analysis of mycobacterial glycolipid

• Project/Area Number: 20790347
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MIYAMOTO Yuji National Institute of Infectious Diseases, ハンセン病研究センター感染制御部, 主任研究官 (40392328)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 抗酸菌 / 糖脂質 / 糖鎖 / 生合成

Research Abstract

Mycobacterium avium complex contains the glycopeptidolipid as one of major components of cell envelope. This study revealed the glycosylation pathways of some specific glycopeptidolipids showing high pathogenicity and that their oligosaccharide portion determined biochemical properties of cell envelope, which was associated with pathogenicity.

Research Products

• [Journal Article] Analysis of drug-resistant strains of Mycobacterium leprae in an endemic area of Vietnam. (2010)
  • Author(s): Kai, M, N.H.Nguyen Phuc, A.H.Nguyen, B.D.H.Pham Thi, K.H.Nguyen, Y.Miyamoto, Y.Maeda, Y.Fukutomi, N.Nakata, M.Matsuoka, M.Masahiko, T.T.Nguyen.
  • Journal Title: Clin. Infect.Dis. 52 Pages: 127-132
• [Journal Article] Influence of advanced age on Mycobacterium bovis BCG vaccination in guinea pigs aerogenically infected with Mycobacterium tuberculosis. (2010)
  • Author(s): Komine-Aizawa, S., T.Yamazaki, T.Yamazaki, S.Hattori, Y.Miyamoto, N.Yamamoto, S.Haga, M.Sugitani, M.Honda, S.Hayakawa, S.Yamamoto.
  • Journal Title: Clin. Vaccine Immunol. 17 Pages: 1500-1506
• [Journal Article] Novel rhamnosyltransferase involved in biosynthesis of serovar 4-specific glycopeptidolipid from Mycobacterium avium complex. (2010)
  • Author(s): Miyamoto, Y., T.Mukai, T.Naka, N.Fujiwara, Y.Maeda, M.Kai, S.Mizuno, I.Yano, M.Makino.
  • Journal Title: J.Bacteriol. 192 Pages: 5700-5708
• [Journal Article] Novel rhamnosyltransferase involved in biosynthesis of serovar 4-specific glycopeptidolipid from Mycobacterium avium complex. (2010)
  • Author(s): 宮本友司、向井徹、中崇、藤原永年、前田百美、甲斐雅規、水野浄子、矢野郁也、牧野正彦
  • Journal Title: The Journal of Bacteriology Volume: 192 Pages: 5700-5708
  • Peer Reviewed
• [Journal Article] Detection of serum antibodies to M.leprae Major Membrane Protein-II in leprosy patients from Indonesia. (2009)
  • Author(s): Hatta M., M.Makino, M.Ratnawati, Mashudi, Yadi, M.Sabir, N.Tandirogang, L.M.M., Rusyati, M.Kai, Y.Fukutomi, Y.Miyamoto, T.Mukai, Y.Maeda.
  • Journal Title: Leprosy Review. 80 Pages: 402-409
• [Journal Article] CD4+ T-cell activation by antigen-presenting cells infected with urease-deficient recombinant Mycobacterium bovis bacillus Calmette-Guerin. (2008)
  • Author(s): Mukai T., Y.Maeda, T.Tamura, Y.Miyamoto, M.Makino.
  • Journal Title: FEMS Immunol.Med.Microbiol. 53 Pages: 96-106
• [Journal Article] Serological diagnosis of leprosy in patients in vietnam by enzyme-linked immunosorbent assay with Mycobacterium leprae-derived major membrane protein II. (2008)
  • Author(s): Kai, M., Phuc N.H.Nguyen, Thi T.H.Hoang, A.H.Nguyen, Y.Fukutomi, Y.Maeda, Y.Miyamoto, T.Mukai, T.Fujiwara, T.T.Nguyen, M.Makino.
  • Journal Title: Clin.Vaccine Immunol. 15 Pages: 1755-1759
• [Journal Article] The Mycobacterium avium complex gtfTB gene encodes a glucosyltransferase required for the biosynthesis of serovar 8-specific glycopeptidolipid. (2008)
  • Author(s): Miyamoto, Y., T.Mukai, Y.Maeda, M.Kai, T.Naka, I.Yano, M.Makino.
  • Journal Title: J.Bacteriol. 190 Pages: 7918-7924
• [Journal Article] The Mycobacterium avium complex gtfTB gene encodes a glucosyltransferase required for the biosynthesis of serovar 8-specific glycopeptidolipid. (2008)
  • Author(s): 宮本友司, Miyamoto Yuji
  • Journal Title: Journal of Bacteriology 190 Pages: 7918-7924
  • Peer Reviewed
• [Presentation] Characterization of the glycopeptidolipid biosynthesis in Mycobacterium avium complex serovar 20.U.S.-Japan Cooperative Medical Science Program. (2010)
  • Author(s): Miyamoto, Y., M.Makino.
  • Organizer: 45^<th> Tuberculosis and Leprosy Research Conference.
  • Place of Presentation: Boston, United States of America
  • Year and Date: 2010-07-14
• [Presentation] Characterization of the glycopeptidolipid biosynthesis in Mycobacterium avium complex serovar 20 (2010)
  • Author(s): 宮本友司、牧野正彦
  • Organizer: U.S.-Japan Cooperative Medical Science Program, 45^<th> Tuberculosis and Leprosy Research Conference
  • Place of Presentation: ブロード研究所(アメリカ合衆国、マサチューセッツ州、ケンブリッジ市)
  • Year and Date: 2010-07-14
• [Presentation] 抗酸菌ファージプロモーターによるらい菌の蛍光蛋白発現 (2010)
  • Author(s): 向井徹, 松岡正典, 前田百美, 宮本友司, 福富康夫, 牧野正彦
  • Organizer: 第83回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-05-28
• [Presentation] 次世代シークエンス解析によるらい菌株のゲノム配列比較 (2010)
  • Author(s): 甲斐雅規, 松岡正典, 宮本友司, 牧野正彦
  • Organizer: 第83回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-05-28
• [Presentation] Mycobacterium avium complex血清型4型株におけるglycopeptidolipidの生合成解析 (2010)
  • Author(s): 宮本友司
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川県横浜市西区パシフィコ横浜
  • Year and Date: 2010-03-27
• [Presentation] Biosynthesis of serovar 4-specific glycopeptidolipid from Mycobacterium avium Complex. US-Japan Cooperative Medical Science Program. (2009)
  • Author(s): Miyamoto, Y., M.Makino.
  • Organizer: 44^<th> Tuberculosis and leprosy Research Conference
  • Place of Presentation: 福岡
  • Year and Date: 2009-07-30
• [Presentation] Major Membrane Protein-IIを用いた血清診断法 (2009)
  • Author(s): 前田百美, Mochammad Hatta, Ratnawati, Mashudi, Yadi, Muhammad Sabir, Nataniel Tandirogang, Luh Made Mas Rusyati, 甲斐雅規, 向井徹, 宮本友司, 福富康夫, 牧野正彦
  • Organizer: 第82回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 出雲
  • Year and Date: 2009-05-14
• [Presentation] らい菌蛋白調整に用いる発現用プロモーターの同定 (2009)
  • Author(s): 向井徹, 宮本友司, 前田百美, 牧野正彦
  • Organizer: 第82回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 出雲
  • Year and Date: 2009-05-14
• [Presentation] Mycobacterium avium complexにおけるglycopeptidolipid生合成遺伝子群の転写制御解析 (2009)
  • Author(s): 宮本友司、向井徹、中崇、甲斐雅規、前田百美、矢野郁也、牧野正彦
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] BCG菌のミコール酸サブクラス合成遺伝子の解析 (2009)
  • Author(s): 甲斐雅規、藤原永年、宮本友司、向井徹、矢野郁也、牧野正彦
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] Mycobacterium avium complexにおけるglycopeptidolipid生合成遺伝子群の転写制御解析 (2009)
  • Author(s): 宮本友司
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋市熱田区名古屋国際会議場
  • Year and Date: 2009-03-13
• [Presentation] Characterization of the glycopeptidolipid biosynthesis in Mycobacterium avium complex serovar 8. US-Japan Cooperative Medical Science Program. (2008)
  • Author(s): Miyamoto, Y., Mukai, T., Maeda, Y., Makino, M.
  • Organizer: 43^<rd> Tuberculosis and leprosy Research Conference.
  • Place of Presentation: Baltimore, United States of America
  • Year and Date: 2008-07-08
• [Presentation] 常温輸送臨床検体のLAMP法によるらい菌遺伝子検出 (2008)
  • Author(s): 向井徹、和泉眞藏、Teky Budiawan、宮本友司、Cita Rosita、Indropo Agusni、松岡正典、牧野正彦
  • Organizer: 第81回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2008-05-23
• [Presentation] らい菌由来免疫原性タンパク、MMP-IIを用いた血清診断 (2008)
  • Author(s): 甲斐雅規、前田百美、福富康夫、宮本友司、向井徹、牧野正彦
  • Organizer: 第81回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 熊本
  • Year and Date: 2008-05-23
• [Remarks] ホームページ等