Identification of host factor using an influenza virus replicon system in yeast cells
| Project/Area Number |
20790349
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
NAITO Tadasuke University of Tsukuba, 大学院・人間総合科学研究科, 助教 (50455937)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | インフルエンザウイルス / 宿主因子 / 酵母 / ゲノム複製 / 転写 / 逆遺伝学 / RNAウイルス |
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| Research Abstract |
To identify host factors systematically, we tried to develop a system in which yeast cells support the replication and transcription of the influenza virus genome depending on transfected vRNP complexes. Yeast is a good model eukaryotic cell with merits including the well-established genetics and the entire genome information for the genome-wide screening. Using a genome-wide set of yeast single-gene deletion strains, we fished several host factor candidates affecting viral RNA synthesis. We found that among them, Tat-SF1 and Prp18 were a stimulatory host factor in the influenza virus RNA synthesis. Tat-SF1 and Prp18 interacted with NP free of RNP that but not with associated with RNA, and facilitates formation of RNA-NP complexes.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Molecular cloning and characterization of porcine Mx2 gene2009
Author(s)
Morozumi T, Naito T, Lan PD, Nakajima E, Mitsuhashi T, Mikawa S, Hayashi T, Awata T, Uenishi H, Nagata K, Watanabe T, Hamasima N
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Journal Title
Molecular Immunology Vol.46
Pages: 858-865
Related Report
Peer Reviewed
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