Visualization of influenza virus progeny RNP complex and analysis of its nucleus-to-plasma membrane transport mechanisms.

• Project/Area Number: 20790358
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Kitasato University
• Principal Investigator: MOMOSE Fumitaka Kitasato University, 大学院・感染制御科学府, 講師 (90332204)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 共焦点レーザー顕微鏡 / インフルエンザウイルス / 微小管 / 宿主因子 / モノクローナル抗体 / ライブセルイメージング / タンパク質トランスフェクション / RNP複合体 / 細胞内輸送 / 微小管輸送 / タンパク質導入 / 極性輸送 / モータータンパク質

Research Abstract

Influenza virus genome is replicated in the nucleus as ribonucleoprotein complex (vRNP) and is transported to the cell surface where the budding of progeny virion occurs. This research revealed that the trafficking of progeny vRNP depends on the host cellular microtubule transport system. Live cell imaging of progeny vRNP demonstrated that the vRNP signals moved along microtubules rapidly (the average velocity of 0.7-1.5μm/s) but intermittently in both plus and minus directions.

Research Products

• [Presentation] インフルエンザウイルス感染細胞におけるHAの細胞内輸送経路の解析 (2010)
  • Author(s): 大倉喬、百瀬文隆、森川裕子
  • Organizer: 第8回感染症沖縄フォーラム
  • Place of Presentation: 沖縄
  • Year and Date: 2010-02-12
• [Presentation] Live-cell imaging of the cytoplasmic trafficking of influenza viral ribonucleoprotein complexes. (2009)
  • Author(s): Fumitaka Momose, Tetsuya Sekimoto, Yuko Morikawa
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Live-cell imaging of the cytoplasmic trafficking of influenza viral ribonucleoprotein complexes. (2009)
  • Author(s): Fumitaka Momose
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2009-12-10
• [Presentation] インフルエンザウイルスRNAゲノムータンパク質複合体輸送の生細胞観察 (2009)
  • Author(s): 百瀬文隆
  • Organizer: 第47回日本生物物理学会年会招待講演
  • Place of Presentation: アスティとくしま(徳島)
  • Year and Date: 2009-10-30
• [Presentation] 生細胞観察によるインフルエンザウイルス子孫RNAゲノム-タンパク質複合体の細胞質内輸送機構の解析 (2009)
  • Author(s): 百瀬文隆、関本哲也、森川裕子
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-27
• [Presentation] 生細胞観察によるインフルエンザウイルス子孫RNAゲノムータンパク質複合体の細胞質内輸送機構の解析 (2009)
  • Author(s): 百瀬文隆
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 2009-10-27
• [Presentation] H5N1亜型トリインフルエンザウイルスHAに対する中和抗体のエピトープ解析とその一本鎖抗体の作製 (2009)
  • Author(s): 大倉喬、菊池雄士、駒瀬勝啓、百瀬文隆、森川裕子
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-26
• [Presentation] Live-cell imaging of the trafficking of influenza viral ribonucleoprotein complexes. (2009)
  • Author(s): Fumitaka Momose, Tetsuya Sekimoto, Yuko Morikawa
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Hyogo, JAPAN
  • Year and Date: 2009-09-09
• [Presentation] Live-cell imaging of the trafficking of influenza viral ribonucleoprotein complexes (2009)
  • Author(s): Fumitaka Momose
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫)
  • Year and Date: 2009-09-09
• [Presentation] インフルエンザウイルスRNP複合体輸送のライブセルイメージング (2008)
  • Author(s): 百瀬文隆
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-12
• [Presentation] インフルエンザウイルスRNP複合体のプラスミドトランスフェクションによる再構成と核外輸送機構の解析 (2008)
  • Author(s): 百瀬文隆
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-26
• [Presentation] Live-cell imaging of influenza viral RNP trafficking (2008)
  • Author(s): Fumitaka Momose
  • Organizer: The 8th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県淡路市
  • Year and Date: 2008-09-10
• [Presentation] Functional analysis of the amino-terminal region of influenza A virus nucleoprotein that participates in RAF-2p48/UAF56 interaction (2008)
  • Author(s): Fumitaka Momose
  • Organizer: 14th International Congress of Virology
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2008-08-12
• [Remarks]
  • URL: http://www.kitasato-u.ac.jp/lisci/