Reverse genetics for rotavirus and its applications

• Project/Area Number: 20790360
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Fujita Health University
• Principal Investigator: KOMOTO Satoshi Fujita Health University, 医学部, 講師 (60367711)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ロタウイルス / ウイルス工学 / リバースジェネティクス / VP4 / ロタイルス / リバースジェネイクテス / ヘルパウイールス

Research Abstract

Recombinant rotaviruses (RVs) containing a cDNA-derived VP4 genome could be generated by means of reverse genetics system. It is expected that this approach will be valuable for the development of novel RV vaccines and vaccine vectors, as well as for an understanding of the molecular basis of RV pathogenesis.

Research Products

• [Journal Article] Generation of recombinant rotavirus with an antigenic mosaic of cross-reactive neutralization epitopes on VP4. (2008)
  • Author(s): Komoto, S., Kugita, M., Sasaki, J., Taniguchi, K.
  • Journal Title: J. Virol. 82 Pages: 6753-6757
  • Peer Reviewed
• [Journal Article] Generation of recombinant rotavirus with an antigenic mosaic of cross- reactive neutralization epitopes on VP4 (2008)
  • Author(s): Satoshi Komoto
  • Journal Title: Journal of Virology 82 Pages: 6753-6757
  • Peer Reviewed
• [Presentation] Proteolytic cleavage at the conserved Arginine-241 of Rotavirus VP4 is not essential for virus replication in cell culture. (2009)
  • Author(s): Komoto, S., Taniguchi, K.
  • Organizer: The 9th Awaji International Forum on Infection and Immunit
  • Place of Presentation: Hyogo, Japan
  • Year and Date: 2009-09-09
• [Presentation] Proteolytic cleavage at the conserved Arginine-241 of Rotavirus VP4 is notessential for virus replication in cell culture (2009)
  • Author(s): Satoshi Komoto
  • Organizer: The 9th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫)
  • Year and Date: 2009-09-09
• [Presentation] VP4上の交叉反応性中和エピトープの抗原モザイクを有する組換えロタウイルスの作製 (2008)
  • Author(s): 河本聡志、釘田雅則、油井晶子、佐々木潤、守口匡子、前野芳正、石川球美子、谷口孝喜
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-28
• [Presentation] ロタウイルスの新知見 (2008)
  • Author(s): 河本聡志
  • Organizer: 第20回ウイルス性下痢症研究会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-25
• [Presentation] Generation of recombinant rotavirus with an antigenic mosaic of cross-reactive neutralization epitopes on VP4. (2008)
  • Author(s): Komoto, S., Kugita, M., Yui, A., Sasaki, J., Taniguchi, K.
  • Organizer: The 8th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Hyogo, Japan
  • Year and Date: 2008-09-09
• [Presentation] Generation of recombinant rotavirus having an antigenic mosaic of cross-reactive neutralization epitopes on VP4. (2008)
  • Author(s): Komoto, S., Kugita, M., Yui, A., Sasaki, J., Moriguchi, K., Maeno, Y., Taniguchi, K.
  • Organizer: XIV International Congress of Virology
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2008-08-13
• [Presentation] Generation of recombinant rotavirus having an antigenic mosaic of cross-reactive neutralization epitopes on VP4. (2008)
  • Author(s): Komoto, S., Kugita, M., Yui, A., Sasaki, J., Taniguchi, K.
  • Organizer: The 42nd Joint Working Conference on Viral Diseases, The Japan-United States Cooperative Medical Science Program
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 2008-05-27
• [Presentation] Generation of recombinant rotavirus having an antigenic mosaic of cross- reactive neutralization epitopes on VP4 (2008)
  • Author(s): Satoshi Komoto
  • Organizer: The 42nd Joint Working Conference on Viral Diseases. The Japan- United States Cooperative Medical Science Program
  • Place of Presentation: 長崎
  • Year and Date: 2008-05-27
• [Book] ロタウイルス感染性クローンの開発と病原性解析 (2009)
  • Author(s): 河本聡志、谷口孝喜
  • Publisher: 共立出版
• [Book] ロタウイルスのリバースジェネティクスとその展望 (2009)
  • Author(s): 谷口孝喜、河本聡志、佐々木潤、釘田雅則
  • Publisher: 日本ウイルス学会
• [Remarks] 研究室ホームページ
  • URL: http://www.fujita-hu.ac.jp/~virology/index.htm
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/~virology/index.htm
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/~virology/index.htm