Regulation of leukocyte trafficking and immune responses by nepmucin

• Project/Area Number: 20790374
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: UMEMOTO Eiji Osaka University, 医学系研究科, 助教 (90452440)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 接着分子 / 免疫学 / 白血球動態 / 免疫応答 / 高内皮細静脈

Research Abstract

Nepmucin is an Ig-domain-containing adhesion molecule expressed in certain types of vascular endothelial cells. We found that nepmucin is localized to interendothelial contacts and mediates homotypic cell adhesion. Nepmucin showed enhanced lymphocyte transendothelial migration by its Ig domain. We also found that nepmucin mediates uptake of apoptotic cells by the interaction of phosphatidylserine, which appears on the outer leaflet of the plasma membrane in apoptotic cells. These results indicate that nepmucin plays multiple physiological roles.

Research Products

• [Journal Article] Microanatomy of lymphocyte-endothelial interactions at the high endothelial venules of lymph nodes (2010)
  • Author(s): Tohya K., Umemoto E., Miyasaka M.
  • Journal Title: Histol. Histopathol 25 Pages: 781-794
  • Peer Reviewed
• [Journal Article] Microanatomy of lymphocyte-endothelial interactions at the high endothelial venules of lymph nodes (2010)
  • Author(s): Tohya K., Umemoto E., et al
  • Journal Title: Histology and Histopathology (In press)
  • Peer Reviewed
• [Journal Article] Involvement of the lysophosphatidic acid-generating enzyme autotaxin in lymphocyte-endothelial cell interactions (2008)
  • Author(s): Nakasaki T. Tanaka, T. Okudaira, S., Hirosawa, M., Umemoto, E., Otani, K., Jin, S., Bai, Z., Hayasaka, H., Fukui, Y., Aozasa, K., Fujita, N., Tsuruo, T., Ozono, K., Aoki, J., Miyasaka, M.
  • Journal Title: Am. J. Pathol 173 Pages: 1566-1576
  • Peer Reviewed
• [Journal Article] Nepmucin/CLM-9, an Ig domain-containing sialomucin in vascular endothelial cells, promotes lymphocyte transendothelial migration in vitro (2008)
  • Author(s): Jin S., *Umemoto E., et al.
  • Journal Title: FEBS Lett 582 Pages: 3018-3024
  • Peer Reviewed
• [Journal Article] Identification of novel isoforms of mouse L-selectin with different carboxyl-terminal tails (2008)
  • Author(s): Furukawa Y., Umemoto E. Jang, M.H., Tohya, K., Miyasaka, M., Hirata, T
  • Journal Title: J. Boil. Chem. 283 Pages: 12112-12119
• [Journal Article] Involvement of the lysophosphatidic acid-generating enzyme autotaxin in lymphocyte-endothelial cell interactions (2008)
  • Author(s): Nakasaki T., et.al.
  • Journal Title: The American Journal of Pathology 173 Pages: 1566-1576
  • Peer Reviewed
• [Journal Article] Nepmucin/CLM-9, an lg domain-containing sialomucin in vascular endothelial cells, promotes lymphocyte transendothelial migration in vitro (2008)
  • Author(s): Jin S., Umemoto E., et.al.
  • Journal Title: FEBS Letters 582 Pages: 3018-3024
  • Peer Reviewed
• [Journal Article] Identification of novel isoforms of mouse L-selectin with different carboxyl-terminal tails (2008)
  • Author(s): Furukawa Y., Umemoto E., et.al.
  • Journal Title: The Journal of Biological chemistry 283 Pages: 12112-12119
  • Peer Reviewed
• [Presentation] Identification of nepmucin as a phosphatidylserine receptor (2009)
  • Author(s): Umemoto E
  • Organizer: 17^<th> International Symposium on Molecular Cell Biology of Macrophages 2009
  • Place of Presentation: 金沢
  • Year and Date: 2009-06-04
• [Presentation] Nepmucin mediates uptake of apoptotic cells in endothelial cells of high endothelial venules (2009)
  • Author(s): 梅本英司, 他
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
• [Presentation] Identification of nepmucin as a phosphatidylserine receptor. 17th International Symposium on Molecular (2009)
  • Author(s): Umemoto, E
  • Organizer: Cell Biology of Macrophages 2009
  • Place of Presentation: Kanazawa
• [Presentation] Nepmucin mediates uptake of apoptotic cells through binding of phosphatidylserine (2008)
  • Author(s): 國澤啓司、梅本英司, ら
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-12-03
• [Presentation] Nepmucin/CLM-9, an lg domain-containing sialomucin in vascular endothelial cells, promotes lymphocyte transendothelial migration (2008)
  • Author(s): 陳秀晶、梅本英司, ら
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-12-01
• [Presentation] Nepmucin/CLM-9, an Ig domain-containing sialomucin in vascular endothelial cells, promotes lymphocyte transendothelial migration (2008)
  • Author(s): 陳秀晶、梅本英司, 他
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] Nepmucin mediates uptake of apoptotic cells through binding of phosphatidylserine (2008)
  • Author(s): 國澤啓司、梅本英司, 他
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都