The investigation of cytosolic uptake mechanisms for basic amino acid-rich peptides to the effective drug delivery

Research Project

Project/Area Number	20790404
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Applied pharmacology
Research Institution	Otani Womens University
Principal Investigator	OKUDA Akiko Otani Womens University, 薬学部, 助教 (60454584)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	ドラッグデリバリー / 塩基性ペプチド
Research Abstract	The endocytic pathways has recently been demonstrated in the major cellular uptake of the basic amino acids-rich peptides, including HIV-1 Tat and oligoarginine. But the cellular uptake into the cytosol has not been demonstrated by real time monitoring. In this study, I established the detection system of the peptides into the cytosol using a split Kusabira Green complementation assay. The fluorescence derived from the Kusabira Green could be observed by confocal laser scanning microscopy in whole cell by adding the oligoarginine-Kusabira Green fragment to the cell culture media for 5-8 hours.

Report

(3 results)