Rapid detection of alteration of outer membrane proteins conferring antimicrobial resistance in Pseudomonas aeruginosa and application to surveillance

• Project/Area Number: 20790413
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Tottori University
• Principal Investigator: HORII Toshinobu Tottori University, 医学部, 准教授 (80283430)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 臨床微生物学 / 緑膿菌 / カルバペネム系薬 / 外膜タンパク質 / 抗菌薬耐性 / OprD / OprM

Research Abstract

Rapid detection of OprD alteration conferring carbapenem resistance in Pseudomonas aeruginosa was developed. To survey carbapenem resistance caused by OprD alteration, carbapenem-resistant P. aeruginosa isolates were collected from all over the country and detected the alteration using our newly developed methods.

Research Products

• [Journal Article] Blood stream infections caused by Aoinetobacter ursiingii in an obstetrics ward (2011)
  • Author(s): Horii T, Tamai K, Mitsui M, Notake S, Yanagisawa H,
  • Journal Title: Infection, Genetics and Evolution 11 Pages: 52-56
  • Peer Reviewed
• [Journal Article] Blood stream infections caused by Acinetobacter ursingii in an obstetrics ward. (2011)
  • Author(s): Horii T, et al.
  • Journal Title: Infection, Genetics and Evolution Volume: 11 Pages: 52-56
  • Peer Reviewed
• [Journal Article] 血液培養検査で繰り返しRhodooooous equiを分離した1症例 (2010)
  • Author(s): 太田博美, 藤原弘光, 田中祐子, 谷本綾子, 堀井俊伸, 上山潤一, 岡崎俊朗
  • Journal Title: 日本臨床微生物学雑誌 20 Pages: 38-43
  • Peer Reviewed
• [Journal Article] Use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens (2009)
  • Author(s): Horii T, Ohtsuka H, Osaki M, Ohkuni H
  • Journal Title: Letters in Applied Microbiology 49 Pages: 46-52
  • Peer Reviewed
• [Journal Article] Detection of carbapenem resistance in clinical mucoid Pseudomonas aeruginosa isolates (2009)
  • Author(s): Horii T, Adachi A, Morita M
  • Journal Title: Scandinavian Journal of Infectious Diseases 41 Pages: 873-876
  • Peer Reviewed
• [Journal Article] Effect of the inoculum size on carbapenem susceptibilities of β-lactamase- negative (2009)
  • Author(s): Miyazaki H, Horii T, Nagura O, Suda T, Chida K, Nakamura H
  • Journal Title: ampicillin-resistant Haemophilus influenzae, Current Microbiology 58 Pages: 18-24
  • Peer Reviewed
• [Journal Article] Clinical experience in a university hospital in Japan (2009)
  • Author(s): Takayama T, Ito T, Suzuki K, Ushiyama T, Horii T, Miura K, Ozono S, BK virus nephropathy
  • Journal Title: International Journal of Urology 16 Pages: 924-929
  • Peer Reviewed
• [Journal Article] Use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens. (2009)
  • Author(s): Horii T, et al.
  • Journal Title: Letters in Applied Microbiology 49 Pages: 46-52
  • Peer Reviewed
• [Journal Article] Detection of carbapenem resistance in clinical mucoid Pseudomonas aeruginosa isolates. (2009)
  • Author(s): Horii T, et al.
  • Journal Title: Scandinavian Journal of Infectious Diseases 41 Pages: 873-876
  • Peer Reviewed
• [Journal Article] BK virus nephropathy : Clinical experience in a university hospital in Japan. (2009)
  • Author(s): Takayama T, et al.
  • Journal Title: International Journal of Urology 16 Pages: 924-929
  • Peer Reviewed
• [Journal Article] Effect of the inoculum size on carbapenem susceptibilities of piactamase-negative, ampicillin-resistant Haemophilus influenzae (2009)
  • Author(s): Miyazaki H., et.al.
  • Journal Title: Current Microbiology 58 Pages: 18-24
  • Peer Reviewed
• [Journal Article] Fluoroquinolone resistance in clinical isolates of Klebsiella oxytoca (2008)
  • Author(s): Horii T, Osaki M, Muramatsu H
  • Journal Title: Chemotherapy 54 Pages: 323-327
  • Peer Reviewed
• [Journal Article] Fluoroquinolone resistance in clinical isolates of Klebsiella oxytoca (2008)
  • Author(s): Horii T., et.al.
  • Journal Title: Chemotherapy 54 Pages: 323-327
  • Peer Reviewed
• [Journal Article] 特集・各種薬物の処方と注意点(3)-その他の領域. 抗菌薬 (2008)
  • Author(s): 堀井俊伸
  • Journal Title: 医学と薬学 60 Pages: 594-604
• [Presentation] シンポジウム:手術部の安全:術後感染症の防止 (2010)
  • Author(s): 堀井俊仲
  • Organizer: 第47回日本麻酔科学会中国・四国支部学術集会
  • Place of Presentation: 米子
  • Year and Date: 2010-09-11
• [Presentation] 手術部の安全:術後感染症の防止 (2010)
  • Author(s): 堀井俊伸
  • Organizer: 第47回日本麻酔科学会中国・四国支部学術集会
  • Place of Presentation: 米子市
  • Year and Date: 2010-09-11
• [Presentation] 手指衛生のコンプライアンスを改善するための取り組み-部門の特徴を捉えた取り組み- (2010)
  • Author(s): 上灘紳子, 堀井俊伸, シンポジウム
  • Organizer: 第25回日本環境感染学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-05
• [Presentation] 遺伝子検査の現状と未来:抗菌薬耐性を制御するための遺伝子検査とこれからの感染症診療 (2009)
  • Author(s): 堀井俊仲, シンポジウム
  • Organizer: 第54回日本臨床検査医学会中国・四国支部総会・第149回日本臨床化学会中国支部例会・総会・第19回日本臨床化学会四国支部例会・総会・第5回合同地方会
  • Place of Presentation: 岡山
  • Year and Date: 2009-02-07
• [Presentation] Antimicrobial susceptibility testings against clinical mucoid Pseudomonas aeruginosa isolates (2009)
  • Author(s): Horii T
  • Organizer: 26th International Congress of Chemotherapy and Infection
  • Place of Presentation: Toronto
• [Presentation] 抗菌薬耐性の制御に向けて～抗菌薬療法をどう適切に進めるか～ (2008)
  • Author(s): 堀井俊伸, セミナー
  • Organizer: 第78回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 広島
  • Year and Date: 2008-12-05
• [Presentation] Fluoroquinolone resistance in clinical isolates of Klebsiella oxytoca (2008)
  • Author(s): Horii T, Mai Osaki
  • Organizer: 13th International Conference on Infectious Diseases
  • Place of Presentation: Kuala Lumpur
• [Presentation] Fluoroquinolone resistance in clinical isolates of Klebsiella oxytoca (2008)
  • Author(s): Horii T, et.al.
  • Organizer: 13th International Conference on Infectious Diseases
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Book] Chapter 3.8. Utility and availability of plasma exposure in healthcare facilities. Akikazu Sakudo and Hideharu Shintani, eds : Sterilization and disinfection by plasma : sterilization mechanisms, biological and medical applications (2011)
  • Author(s): Horii T
  • Publisher: Nova Science Publishers