Establishment of new pathological analysis by imaging mass spectrometry

• Project/Area Number: 20790419
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: ZAIMA Nobuhiro Hamamatsu University School of Medicine, 分子イメージング先端研究センター, 特任助教 (40455572)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 質量顕微鏡法 / 病理解析 / 脂質代謝異常 / 質量顕微鏡 / 脂質 / 脂質蓄積ミオパチー / 脂肪肝

Research Abstract

Biochemical approaches, such as staining, are widely used as pathological analysis. These methods are essential for pathological analysis. However, they have some disadvantages. For example, they cannot be applied to non-targeting analysis. Establishment of new pathological method is important to analyze idiopathic disease. In this study, new pathological analysis using imaging mass spectrometry for non-targeting analysis was established.

Research Products

• [Journal Article] Principal component analysis of direct matrix-assisted laser desorption/ionization mass spectrometric data related to metabolites of fatty liver (2009)
  • Author(s): Zaima N, Matsuyama Y, Setou M
  • Journal Title: J Oleo Sci 58 Pages: 267-273
  • NAID: 130000106453
  • Peer Reviewed
• [Journal Article] Imaging of metabolites by MALDI mass spectrometry (2009)
  • Author(s): Zaima N, Hayasaka T, Goto-Inoue N, Setou M
  • Journal Title: J Oleo Sci 58 Pages: 415-419
  • NAID: 130000126093
  • Peer Reviewed
• [Journal Article] Imaging of metabolites by MALDI mass spectrometry (2009)
  • Author(s): Nobuhiro Zaima
  • Journal Title: Journal of Oleo Science 58 Pages: 415-419
  • NAID: 130000126093
  • Peer Reviewed
• [Journal Article] Principal component analysis of direct matrix-assisted laser desorption/ionization mass spectrometric data related to metabolites of fatty liver (2009)
  • Author(s): Nobuhiro Zaima
  • Journal Title: Journal of Oleo Science (In press)
  • NAID: 130000106453
  • Peer Reviewed
• [Presentation] The abnormal accumulation of metabolites in neutral lipid storage myopathy caused by adipose triacylglycerol lipase mutation. (2009)
  • Author(s): Zaima, N., Sugiura, Y., Hirano, K., Ohkuma, A., Okahashi, S., Noguchi, S., Nishino, I., Ibi, T., Sahashi, K., Setou, M.
  • Organizer: The American Oil Chemists' Society
  • Place of Presentation: USA
  • Year and Date: 2009-05-04
• [Presentation] The pathological analysis of neutral lipid storage myopathy caused by a dipose triacylglycerol lipase mutation. (2009)
  • Author(s): Nobuhiro Zaima
  • Organizer: The American Oil Chemists' Society
  • Place of Presentation: Florida (USA)
  • Year and Date: 2009-05-04
• [Presentation] Analysis of Lipid Storage Myopathy (ETFDH mutant or PNPLA2 mutant) by Direct Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (2008)
  • Author(s): Nobuhiro Zaima
  • Organizer: American Society for Mass Spectrometry
  • Place of Presentation: Denver (USA)
  • Year and Date: 2008-06-05
• [Presentation] Analysis of Lipid Storage Myopathy (ETFDH mutant or PNPLA2 mutant) by Direct Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (2008)
  • Author(s): Zaima, N., Sugiura, Y., Ohkuma, A., Okahashi, S., Noguchi, S., Nishino, I., Ibi, Tohru., Ozawa, F., Sahashi, K., Setou M.
  • Organizer: Conference Proceedings, 56th ASMS Conference on Mass Spectrometry
  • Place of Presentation: USA
  • Year and Date: 2008-05-06