| Project/Area Number |
20790461
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
IKEMATSU Kazuya Nagasaki University, 大学院・医歯薬学総合研究科, 准教授 (80332857)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 法医病理学 / 蛍光2次元電気泳動法 / マウス小脳 / 頚部圧迫 / 窒息 |
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| Research Abstract |
We explored a specific protein which responded to hypoxia on an animal model of asphyxia with 2-Dimentioanl electrophoresis. Mice were anaesthetized after which hypoxia was induced by neck ligation for 30 min, then were killed by decapitation, and the brains were dissected either immediately (H-0) or 60 min (H-60) after death. Control mice without ligation of the neck were killed by decapitation under anaesthesia immediately (C-0) or 60 min (C-60) after death.
In forebrain, about 100 spots were observed. 2 spots were increased in H-0 and H-60. In addition, other 2 spots were increased. In cerebellum, about 100 spots were observed. 2 spots were increased in H-0 and H-60. In addition, other 2 spots were increased.
These increased spots might be asphyxia response proteins.
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