Aiming for the improvement of regenerative ability of the liver from hepatic stem cells by analyzing the role of SDF-1

• Project/Area Number: 20790488
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Niigata University
• Principal Investigator: TSUCHIYA Ataunori Niigata University, 医歯学総合病院, 医員 (70464005)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: SDF-1 / 肝再生 / 肝幹前駆細胞 / CXCR4 / マウス / ALT / Mxマウス / VEGF / 肝臓 / ductular reaction / floxマウス / CD11b

Research Abstract

In the liver, stromal cell derived factor-1 (SDF-1) is produced in the bile duct and hepatic stem/progenitor cells. In this study, we analyzed the role of SDF-1 by using the CXCR4 conditional knock out (KO) mice (CXCR4 is the receptor of SDF-1). By analyzing the liver damaged CXCR4 KO mice, we realized that although in the liver hepatic stem/progenitor cells increased, the recovery of liver regeneration was delayed. These results reveal that the role of SDF-1/CXCR4 axis works positive effect for liver regeneration.

Research Products

• [Journal Article] Successful treatment of multiple lung metastases of hepatocellular carcinoma by combined chemotherapy with docetaxel, cisplatin and tegafur/uracil (2009)
  • Author(s): Tsuchiya A, Imai M, Kamimura H, Togashi T, Watanabe K, Seki K, Ishikawa T, Ohta H, Yoshida T, Kamimura T
  • Journal Title: World J Gastroenterol 15(14) Pages: 1779-1781
• [Journal Article] Successful Treatment in a Case of Massive Hepatocellular Carcinoma with Paraneoplastic Syndrome (2009)
  • Author(s): Tsuchiya A, Kubota T, Takizawa K, Yamada K, Wakai T, Matsuda Y, Honma T, Watanabe M, Shirai Y, Maruyama H, Nomoto M, Aoyagi Y
  • Journal Title: Case reports in gastroenterology 3 Pages: 105-110
• [Journal Article] Clinicopathological analysis of CD133+ and NCAM+ human hepatic stem/progenitor cells in damaged livers and hepatocellular carcinomas (2009)
  • Author(s): Tsuchiya A, Kamimura H, Takamura M, Yamagiwa S, Matsuda Y, Sato Y, Nomoto M, Ichida T, Aoyagi Y
  • Journal Title: Hepatology Research 39 Pages: 1080-1090
• [Journal Article] 劇症肝炎に対する再生医学の現実性-内科医の立場で- (2009)
  • Author(s): 土屋淳紀
  • Journal Title: 肝胆膵 59(3) Pages: 469-476
• [Journal Article] Successful treatment of multiple lung metastases of hepatocellular carcinoma by combined chemotherapy with docetaxel, cisplatin and tegafur/uracil (2009)
  • Author(s): Atsunori Tsuchiya
  • Journal Title: World J Gastroenterol 15(14) Pages: 1779-1781
  • Peer Reviewed
• [Journal Article] Successful Treatment in a Case of Massive Hepatocellular Carcinoma with Paraneoplastic Syndrome (2009)
  • Author(s): Atsunori Tsuchiya
  • Journal Title: Case reports in gastroenterology 3 Pages: 105-110
  • Peer Reviewed
• [Journal Article] Clinicopathological analysis of CD133+ and NCAM+ human hepatic stem/progenitor cells in damaged livers and hepatocellular carcinomas (2009)
  • Author(s): Atsunori Tsuchiya
  • Journal Title: Hepatology Research 39 Pages: 1080-1090
  • Peer Reviewed
• [Presentation] 急性、慢性、人障害肝に見られるNCAM陽性肝幹前駆細胞およびNCAM陽性肝細胞癌の解析 (2009)
  • Author(s): 土屋淳紀
  • Organizer: 第16回肝細胞研究会
  • Place of Presentation: 山形市
  • Year and Date: 2009-06-27
• [Presentation] 人障害肝内に出現する肝幹前駆細胞の分化階層性の解析および肝癌幹細胞マーカーとしての可能性 (2009)
  • Author(s): 土屋淳紀
  • Organizer: 第106回内科学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2009-04-12
• [Presentation] 術前CDDP肝動注療法にて、肝機能を保ちかつ著効し狭心症加療後切除し長期生存した多血症、高脂血症の腫瘍随伴症候群を伴う高齢者巨大肝細胞癌の一例 (2008)
  • Author(s): 土屋淳紀
  • Organizer: 第37回日本肝臓学会東部会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-04
• [Presentation] 急性、慢性障害肝組織中でのCD133, NCAM陽性細胞の出現とその分化階層性の解析 (2008)
  • Author(s): 土屋淳紀
  • Organizer: 第40回臨床分子形態学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-10-03
• [Presentation] 門脈域に存在するSca-1陽性血管内皮細胞は急性肝障害時の早期回復に貢献する (2008)
  • Author(s): 土屋淳紀
  • Organizer: JDDW2008(日本消化器関連学会集会)
  • Place of Presentation: 東京
  • Year and Date: 2008-10-01
• [Presentation] Sca-1+ endothelial cells reside in the portal area of the liver and contribute to rapid recovery from acute liver disease (2008)
  • Author(s): 土屋淳紀
  • Organizer: 6^<th> ISSCR (International Society for Stem Cell Research)
  • Place of Presentation: フィラデルフィア(アメリカ)
  • Year and Date: 2008-06-11
• [Presentation] 人障害肝内に出現する肝幹前駆細胞の分化階層性の解析および肝癌幹細胞マーカーとしての可能性
  • Author(s): 土屋淳紀、高村昌昭、山際訓、野本実、青柳豊
  • Organizer: 内科学会総会
• [Presentation] 急性、慢性、人障害肝に見られるNCAM陽性肝幹前駆細胞およびNCAM陽性肝細胞癌の解析
  • Author(s): 土屋淳紀、野本実、青柳豊
  • Organizer: 肝細胞研究会