Effect of nitorosylated- and phosphorylated-RKIP on cell-proliferation signal in gastric cancer cells

• Project/Area Number: 20790505
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Josai University
• Principal Investigator: OHTAKE Kazuo Josai University, 薬学部, 助教 (50337482)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 癌 / 整理活性 / 蛋白質 / シグナル伝達 / 一酸化窒素 / ニトロソ化 / 細胞増殖 / 翻訳後修飾 / 生理活性 / RKIP / リン酸化 / Raf / ERK

Research Abstract

The purpose of the current study is to evaluate the effect of nitorosylated- and phosphorylated-RKIP (Raf kinase inhibitor protein) on cell-proliferation signal in human gastric cancer (AGS) cells. RKIP-nitrosylation was significant reduction in the phophorylation level of RKIP in AGS cells. On the other hand, cell proliferation signal was up-regulated by an expressive increase in nitrosylation level of RKIP.

Research Products

• [Journal Article] Proteomic approach for identification of protein S-nitrosation in mouse gastric mucosa treated with S-nitrosoglutathione (2009)
  • Author(s): Ohtake K, Shimada N, Uchida N, Kobayashi
  • Journal Title: J. Proteomics 72 Pages: 750-760
  • Peer Reviewed
• [Journal Article] Proteomic approach for identification of protein S-nitrosation in mouse gastric mucosa (2009)
  • Author(s): Ohtake K, Shimada N, Uchida N, Kobayashi
  • Journal Title: Nitric Oxide 20
  • Peer Reviewed
• [Journal Article] Proteomic approach for identification of protein S-nitrosation in mousegastric mucosa treated with S-nitrosoglutathione (2009)
  • Author(s): K.Ohtake, 他3名
  • Journal Title: J Proteomics 72 Pages: 750-760
  • Peer Reviewed
• [Journal Article] Proteomic approach for identification of protein S-nitrosation in mousegastric mucosa (2009)
  • Author(s): K.Ohtake, 他3名
  • Journal Title: Nitric Oxide 20
  • Peer Reviewed
• [Presentation] Proteomic approach for identification of protein S-nitrosation in mouse gastric mucosa (2009)
  • Author(s): K Ohtake, 他3名
  • Organizer: THIRD INTERNATIONAL MEETING ON THE ROLE OF NITRITE IN PHYSIOLOGY, PATHOPHYSIOLOGY, AND THERAPERUTICS
  • Place of Presentation: スウェーデンストックホルムカロリンスカ研究所
  • Year and Date: 2009-06-17
• [Presentation] Proteomic approach for identification of protein S-nitrosationin mouse gastric mucosa (2009)
  • Author(s): K Ohtake, 他3名
  • Organizer: THIRD INTERNATIONAL MEETING ONTHE ROLE OF NITRITE IN PHYSIOLOGY, PATHOPHYSIOLOGY, AND THERAPERUTICS
  • Place of Presentation: スウェーデン ストックホルム カロリンスカ研究所
  • Year and Date: 2009-06-17
• [Presentation] S-ニトロソ化RKIPはRKIPリン酸化を阻害し、ERKリン酸化を活性化する (2009)
  • Author(s): 大竹一男, 他3名
  • Organizer: 第9回日本NO学会学術集会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-05-08
• [Presentation] S-ニトロソ化RKIPはRKIPリン酸化を阻害し、 ERI(リン酸化を活性化する (2009)
  • Author(s): 大竹一男, 他3名
  • Organizer: 第9回日本NO学会学術集会
  • Place of Presentation: 静岡 グランシップ
  • Year and Date: 2009-05-08
• [Presentation] ヒト胃細胞におけるNOストレスはRKIP非依存的にERKをリン酸化する (2009)
  • Author(s): 大竹一男
  • Organizer: 第129回日本薬学会年会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-03-27