The role of autoantigen presentation by autophagy of biliary epitherial cell in the pathogenesis of primary biliary cirrhosis.

• Project/Area Number: 20790515
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: AMANO Katsushi Jikei University School of Medicine, 医学部, 助教 (70424645)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: PBC / 胆管細胞 / オートファジー / 胆管上皮細胞

Research Abstract

The role of autoantigen presentation by autophagy of biliary epitherial cell (BEC) in the pathogenesis of primary biliary cirrhosis was analyzed. Autophagy of BEC was induced by LPS or CpGDNA stimulation and the expression of MHC class II antigen on BEC was induced. PDCE2 specific immunological tolerance was not induced in vitro by immunization of dendritic cell which take autophagy induced BEC. When dendritic cell which take autophagy induced BEC was administered to the animal model of PBC before the bile duct damage was take place, inflammation with bile duct damage was slightly improved compared with control. However, such improvement was not observed when dendritic cell which take autophagy induced BEC was administered after the bile duct damage was take place.,

Research Products

• [Journal Article] Accumulation of functional regulatory T cells in actively inflamed liver in mouse dendritic cell-based autoimmune hepatic inflammation. (2010)
  • Author(s): Saeki C, Nakano M, Takahashi H, Zeniya M, et al.
  • Journal Title: Clin Immunol 135 Pages: 156-166
• [Presentation] Insulin resistance was involved in the pathogenesis of autoimmune hepatitis without the relation to prednisone therapy. (2010)
  • Author(s): Takahashi H, et al.
  • Organizer: 60th Annual Meeting of American Association for the Study of Liver Disease.
  • Place of Presentation: Boston, USA
  • Year and Date: 2010-10-31
• [Presentation] Serum ALT in normal level of healthy individuals positively and independently correlates to the markers of metabolic syndrome. (2010)
  • Author(s): Takahashi H, et al.
  • Organizer: 45th Annual Meeting of Europian Association for the Study of Liver Disease.
  • Place of Presentation: Vienna, Australia
  • Year and Date: 2010-04-18
• [Presentation] Accumulation of functional regulatory T cells in actively inflamed liver in mouse dendritic cell-based autoimmune hepatic inflammation. (2009)
  • Author(s): Saeki C, et al.
  • Organizer: 59th Annual Meeting of American Association for the Study of Liver Disease.
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-11-04
• [Presentation] Increased intrahepatic Foxp3+ Treg may participate in the natural occurring recovery of experimental autoimmune hepatitis. (2008)
  • Author(s): Saeki C, et al.
  • Organizer: 58th Annual Meeting of American Association for the Study of Liver Disease.
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-11-02
• [Presentation] Reciprocal changes of Tr1 and Th17 are involved in the pathogenesis of autoimmune liver diseases. (2008)
  • Author(s): Takahashi H, et al.
  • Organizer: 58th Annual Meeting of American Association for the Study of Liver Disease.
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-11-02