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Development of liver regeneration therapy using of anti-fibrotic gene-modified endothelial progenitor cells

Research Project

Project/Area Number 20790517
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionKurume University

Principal Investigator

NAKAMURA Toru  Kurume University, 医学部, 助教 (30341332)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords血管内皮細胞(EPC) / 細胞療法 / 肝線維化 / 肝硬変症 / 肝再生 / アデノウイルスベクター / 肝細胞増殖因子(HGF) / 血管内皮前駆細胞(EPC) / アデノウイスルベクター / HGF(肝細胞増殖因子)
Research Abstract

Previously we reported that endothelial progenitor cell (EPC) transplantation could reduce established liver fibrosis and promote hepatic regeneration. In the current study, we investigated the hypothesis that gene transfer can be used to achieve phenotypic modulation of EPCs. Especially, we investigated the effect on regenerative properties of human hepatocyte growth factor (hHGF)-transduced EPCs in cirrhotic rat liver. In AdhHGF-transduced group, the degree of liver fibrosis was suppressed compared to those in AdLacZ-transduced group at 10 weeks after CCl_4 treatment. Ki67-positive hepatocytes in AdhHGF-transduced group were significantly increased. Moreover, serum total protein and albumin levels were significantly higher in AdhHGF-transduced group than those in AdLacZ-transduced group. In conclusion, this enhanced regenerative acceleration of transplanted EPCs by hHGF transfer will provide novel therapeutical strategy for hepatic regeneration in severe cirrhotic liver disease patients.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (14 results)

All 2010 2009 2008

All Presentation (12 results) Book (2 results)

  • [Presentation] 血管内皮前駆細胞を用いた肝臓再生療法-基礎研究から臨床研究へ-2010

    • Author(s)
      中村徹
    • Organizer
      第 回九州BMT研究会
    • Place of Presentation
      福岡
    • Year and Date
      2010-02-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Dose-dependent contribution of human peripheral blood CD34-positive cell transplantation to hepatic regeneration after CCl4 chronic liver injury2010

    • Author(s)
      Toru Nakamura, et.al.
    • Organizer
      61th Annual Meeting of The American Association For The study of Liver Disease
    • Place of Presentation
      Boston, MA, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] 健常ヒト末梢血CD34陽性細胞を用いた肝再生医療2010

    • Author(s)
      中村徹、鳥村拓司、佐田通夫
    • Organizer
      第18回日本消化器関連学会週間(JDDW)
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Human peripheral blood CD34-positive cells exhibit increased potency for therapeutic hepatic regeneration in carbon tetrachloride-induced hepatic fibrosis model nude rats2010

    • Author(s)
      Toru Nakamura, et al
    • Organizer
      15th International Symposium on Cells of the Hepatic Sinusoid
    • Place of Presentation
      Pasadena, California, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Human peripheral blood CD34-positive cells exhibit increased potency for therapeutic hepatic regeneration in carbon tetrachloride-induced hepatic fibrosis model nude rats2010

    • Author(s)
      Toru Nakamura, et.al.
    • Organizer
      15^<th> International Symposium on Cells of the Hepatic Sinusoid
    • Place of Presentation
      Pasadena, California, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] 健常ヒト末梢血CD34陽性細胞を用いた肝再生医療2010

    • Author(s)
      中村徹、鳥村拓司、佐田通夫
    • Organizer
      第18回 日本消化器関連学会週間(JDDW)
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dose-dependent contribution of human peripheral blood CD34-positive cell transplantation to hepatic regeneration after CCl_4 chronic liver injury2010

    • Author(s)
      Toru Nakamura, et.al.
    • Organizer
      61^<th> Annual Meeting of The American Association For The study of Liver Disease
    • Place of Presentation
      Boston, MA, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] 肝硬変症に対する血管内皮前駆細胞を用いた肝臓再生療法2009

    • Author(s)
      中村徹、鳥村拓司、他4名
    • Organizer
      日本臨床分子形態学術学会
    • Place of Presentation
      神戸
    • Year and Date
      2009-09-05
    • Related Report
      2010 Final Research Report
  • [Presentation] 肝硬変症に対する血管内皮前駆細胞を用いた肝臓再生療法2009

    • Author(s)
      中村徹, 鳥村拓司, 他4名
    • Organizer
      日本臨床分子形態学術学会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2009-09-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 血管内皮前駆細胞を用いた肝硬変の治療2009

    • Author(s)
      中村徹
    • Organizer
      第1回福岡医学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2009-01-25
    • Related Report
      2010 Final Research Report
  • [Presentation] 血管内皮前駆細胞へのHGF遺伝子導入による肝再生遺伝子治療の試み2008

    • Author(s)
      中村徹、鳥村拓司、他5名
    • Organizer
      第22回肝類洞壁細胞研究会 学術集会
    • Place of Presentation
      久留米
    • Year and Date
      2008-11-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 血管内皮前駆細胞へのHGF遺伝子導入による肝再生遺伝子治療の試み2008

    • Author(s)
      中村徹, 鳥村拓司, 他5名
    • Organizer
      第22回肝類洞壁細胞研究会学術集会
    • Place of Presentation
      久留米
    • Year and Date
      2008-11-29
    • Related Report
      2008 Annual Research Report
  • [Book] アークメディア(肝胆膵血管内皮前駆細胞を用いた肝臓再生療法)、Vol.57、No.32008

    • Author(s)
      中村徹、佐田通夫
    • Related Report
      2010 Final Research Report
  • [Book] 肝胆膵血管内皮前駆細胞を用いた肝臓再生療法2008

    • Author(s)
      中村徹, 佐田通夫
    • Total Pages
      502
    • Publisher
      アークメデイア
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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