Establishment of differentiation method of iPS cell derived cardiomyocytes

• Project/Area Number: 20790549
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: OHNO Yohei Keio University, 医学部, 助教 (80383884)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: iPS細胞 / 患者特異的iPS細胞 / iPS細胞由来心筋細胞 / iPS細胞由来心筋の分化誘導効率 / iPS細胞由来心筋の移植効率

Research Abstract

We analyzed the difference of cardiomyocyte differentiation derived from mouse iPS cells. RT-PCR analysis of the purified iPS-derived cardiomyocytes revealed that iPS-derived cardiomyocytes with higher expression level of residual exogenous transgene had lower beating efficiency compared with that of iPS-derived cariomyocytes with lower expression level of residual exogenous transgene. Higher expression level of exogenous transgene may have contributed to lower incidence of beating colonies, preventing differentiation to cardiomyocytes.

Research Products

• [Presentation] Molecular characterization of induced pluripotent stem (iPS) cell- derived cardiomyocytes (2010)
  • Author(s): Yohei Ohno, Shinsuke Yuasa, Toru Egashira, Tomohisa Seki, Hisayuki Hashimoto, Shugo Toyama, Takahide Arai, Tomofumi Tanaka, Fumiyuki Hattori, Kojiro Yae, Mitsushige Murata, Satoshi Ogawa, Shinya Yamanaka, Keiichi Fukuda
  • Organizer: 日本循環器学会
  • Place of Presentation: 京都
  • Year and Date: 2010-03-06
• [Presentation] Molecular Characterization of Induced Pluripotent Stem (iPS) Cell-Derived Cardiomyocytes (2009)
  • Author(s): Yohei Ohno, Shinsuke Yuasa, Toru Egashira, Tomohisa Seki, Hisayuki Hashimoto, Shugo Toyama, Sung Han Yoon, Takahide Arai, Chen Hao, Tomofumi Tanaka, Fumiyuki Hattori, Kojiro Yae, Mitsushige Murata, Satoshi Ogawa
  • Organizer: American Heart Association
  • Place of Presentation: USA
  • Year and Date: 2009-11-16
• [Presentation] Molecular Characterization of Induced Pluripotent Stem(iPS) Cell-Derived Cardiomyocytes (2009)
  • Author(s): 大野洋平
  • Organizer: American Heart Association
  • Place of Presentation: Orlando, USA
  • Year and Date: 2009-11-16
• [Presentation] Molecular characterization, safety and feasibility of induced pluripotent stem (iPS) cellderived cardiomyocytes for heart regenerative therapy (2009)
  • Author(s): Yohei Ohno, Shinsuke Yuasa, Takeshi Onizuka, Toru Egashira, Kenichiro Shimoji, Sung Han Yoon, Takahide Arai, Chen Hao, Tomofumi Tanaka, Fumiyuki Hattori, Toshimi Kageyama, Satoshi Ogawa, Shinya Yamanaka, Keiichi Fukuda
  • Organizer: 日本循環器学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-03-22
• [Presentation] Molecular Characterization, Safety and Feasibility of Induced Pluripotent Stem(iPS)Cell Derived Cardiomyocytes for Heart Regenerative Therapy (2008)
  • Author(s): 大野洋平
  • Organizer: American Heart Association
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-11-11