Research of cardiac parasympathetic nerves

• Project/Area Number: 20790550
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: ARAI Takahide Keio University, 医学部, 助教 (00383894)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 心臓副交感神経 / 心臓支配副交感神経 / VAChT-creマウス / LIF / ChAT-cre / VAChT-cre / gp130

Research Abstract

It aimed to clarify a molecular mechanism concerning the cardiac parasympathetic nerves in this research. It aimed first making the cardiac parasympathetic nerves visible for that. It was confirmed to be able to dye the cardiac parasympathetic nerves and cardiac ganglia with ChAT(choline acetyl transferase), and confirmed ChAT was dominant on the central side of the heart in the adult mouse. The cre-loxP system aims at Cre recombinase in ChAT in the peculiarity and aims to establish the ChAT-EGFP mouse, and to make cardiac parasympathetic nerves visible from the appearing ChAT-cre mouse.