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Regulatory mechanisms of TRP channel by diacylglycerol kinase in blood vessels.

Research Project

Project/Area Number 20790559
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionHyogo College of Medicine

Principal Investigator

NAKANO Tomoyuki  Hyogo College of Medicine, 医学部, 助教 (00333948)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsジアシルグリセロールキナーゼ / 血管平滑筋細胞 / 血管内皮細胞 / DGK / IL-1β / ゴルジ体 / ストレスファイバー / 免疫細胞化学 / セロトニン
Research Abstract

In the present study, diacylglycerol kinase (DGK) expression in rat aortic cells was investigated. In rat aortic smooth muscle cells (RASMC), α, ε and ζ types of DGK isozymes mRNA were detected. Immunocytochemical analysis revealed that DGKα, ε, ζ were localized in nucleus and cytoplasm, cytoskelton and nucleus, respectively. Phalloidin staining demonstrated that DGK_ε was localized to actin stress fibers. When RASMC were treated with serotonin, a contractile agonist, subcellular localization of DGKε was altered to be diffusely in cytoplasm. These facts indicate that DGK_ε functions in the regulatory mechanism of contraction in RASMC. In rat aortic endothelial cells (RAEC), immunocytochemical analysis revealed that DGK_γ was localized in Golgi complex. Present study demonstrated that up-regulated expression of DGK_γ mRNA by IL-1β. We also investigated the expression of cyclooxygenase-2 (COX-2) which was induced by IL-1β. In endothelial denuded aortae, COX-2 expression was induced in tunica media, in addition to adventitia, whereas no expression was detected in tunica media of intact aortae. These facts can be significant to study functional role of DGK isozymes, for example regulation of TRP channels, in vascular cells.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (17 results)

All 2010 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (11 results)

  • [Journal Article] Identification of cells expressing COX-2 in response to interleukin-1βin rat aortae.2010

    • Author(s)
      T. Nakano, I. Wakabayashi
    • Journal Title

      J. Pharmacol. Sci. 113

      Pages: 84-88

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of interleukin-1 β-induced iNOS expression in cultured vascular smooth muscle cells of Goto-Kakizaki diabetes rats.2010

    • Author(s)
      I. Wakabayashi, T. Nakano, Y. Takahashi
    • Journal Title

      Eur. J. Pharmacol. 629

      Pages: 1-6

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of cells expressing COX-2 in response to interleukin-1β in rat aortae2010

    • Author(s)
      T.Nakano, I.Wakabayashi
    • Journal Title

      Journal of Pharmacological Science (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of interleukin-1β-induced iNOS expression in cultured vascular smooth muscle cells of Goto-Kakizaki diabetes rats2010

    • Author(s)
      I.Wakabayashi, T.Nakano, Y.Takahashi
    • Journal Title

      European Journal of Pharmacology 629

      Pages: 1-6

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization of diacylglycerol kinase s on stress fibers in vascular smooth muscle cells.2009

    • Author(s)
      T. Nakano, Y Hozumi, K. Goto, I. Wakabayashi
    • Journal Title

      Cell Tis. Res. 337

      Pages: 167-175

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Localization of diacylglycerol kinase ε on stress fibers in vascular smooth muscle cells2009

    • Author(s)
      T. Nakano, et.al.
    • Journal Title

      Cell and Tissue Research (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] IL-1β-induced expression of diacylglycerol kinase γ in Golgi complex in rat aortic endothelial cells2009

    • Author(s)
      T.Nakano, K.Goto, I.Wakabayashi
    • Organizer
      49^<th> annual meeting of American Society for Cell Biology
    • Place of Presentation
      San Diego convention center, (San Diego CA, USA)
    • Year and Date
      2009-12-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 2型糖尿病ラット大動脈でのiNOS発現の変化2009

    • Author(s)
      高橋裕二、中野知之、若林一郎
    • Organizer
      第9回日本NO学会
    • Place of Presentation
      グランシップ(静岡県静岡市)
    • Year and Date
      2009-05-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 血管内皮細胞におけるジアシルグリセロールキナーゼ発現の解析-iNos発現制御の可能性-2009

    • Author(s)
      中野知之、後藤薫、若林一郎
    • Organizer
      第38回日本心脈管作動物質学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2009-02-06
    • Related Report
      2009 Final Research Report
  • [Presentation] 血管内皮細胞におけるジアシルグリセロールキナーゼ発現の解析 : iNos発現制御の可能性2009

    • Author(s)
      中野知之, ら
    • Organizer
      第38回日本心脈管作動物質学会
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2009-02-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] IL-1 β-induced expression of diacylglycerol kinaseγin Golgi complex in rat aortic endothelial cells2009

    • Author(s)
      T. Nakano, K. Goto, I. Wakabayashi
    • Organizer
      49th annual meeting of American Society for Cell Biology
    • Place of Presentation
      San Diego CA, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] 血管内皮細胞におけるジアシルグリセロールキナーゼの発現解析2009

    • Author(s)
      中野知之、若林一郎、後藤薫
    • Organizer
      第114回日本解剖学会総会
    • Place of Presentation
      岡山理科大学
    • Related Report
      2009 Final Research Report
  • [Presentation] 血管内皮細胞におけるジアシルグリセロールキナーゼの発現解析2009

    • Author(s)
      中野知之, ら
    • Organizer
      第114回日本解剖学会総会
    • Place of Presentation
      岡山大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] ジアシルグリセロールキナーゼによる血管平滑筋機能制御の可能性2008

    • Author(s)
      中野知之、後藤薫、若林一郎
    • Organizer
      第37回日本心脈管作動物質学会
    • Place of Presentation
      仙台
    • Year and Date
      2008-02-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Expression and subcellular localization of diacylglycerol kinase isozyme in rat aortic endothelial cells2008

    • Author(s)
      T. Nakano, K. Goto, I. Wakabayashi
    • Organizer
      48th Annual meeting of American Society for Cell Biology
    • Place of Presentation
      San Francisco, CA, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] 血管平滑筋細胞におけるジアシルグリセロールキナーゼの発現・機能解析2008

    • Author(s)
      中野知之、若林一郎、後藤薫
    • Organizer
      第113回日本解剖学会総会
    • Place of Presentation
      大分
    • Related Report
      2009 Final Research Report
  • [Presentation] Expression and subcellular localization of diacylglycerol kinase isozyme in rat aortic endothelial cells2008

    • Author(s)
      T. Nakano, et.al.
    • Organizer
      48th American Society for Cell Biology
    • Place of Presentation
      San Francisco, CA, USA
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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