Development of the method to investigate the mechanism of hypertensive remodeling using laser microdissection

• Project/Area Number: 20790560
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Kurume University
• Principal Investigator: IKEDA Ayami Kurume University, 医学部, 助教 (80412500)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 高血圧 / 分子生物学 / 高血圧性臓器障害

Research Abstract

We sought to establish the method to selectively collect the myocardium, vessels, and perivascular infiltrating cells from the heart sections and to compare gene expression profile of each tissue component between normotensive and hypertensive hearts using laser microdissection method. Real-time RT-PCR analysis showed that BNP, a marker of the cardiomyocytes, was expressed in the myocardial samples, but not in the vascular samples, whereas alpha-SMA, a marker of smooth muscle cells, was detected only in the vascular samples. Moreover, BNP was more upregulated in stroke-prone spontaneously hypertensive rats than in WKY rats.

Research Products

• [Journal Article] Exaggerated blood pressure variability superimposed on hypertension aggravates cardiac remodeling in rats via angiotensin II system-mediated chronic inflammation. (2009)
  • Author(s): Kudo H, Ikeda A, (他11 名7 番目)
  • Journal Title: Hypertension 54 Pages: 832-838
• [Journal Article] Exaggerated blood pressure variability superimposed on hypertension aggravates cardiac remodeling in rats via angiotensin II system-mediated chronic inflammation (2009)
  • Author(s): Kudo H, Kai H, (他11名2番目)
  • Journal Title: Hypertension 54 Pages: 832-838
  • Peer Reviewed
• [Journal Article] Mutant MCP-1 Therapy Inhibits Tumor Angiogenesis and Growth of Malignant Melanoma in Mice. (2008)
  • Author(s): Koga M, Ikeda A, (他10 名5 番目)
  • Journal Title: Biochem Biophys Res Comm 365 Pages: 279-284
• [Journal Article] Mutant MCP-1 Therapy Inhibits Tumor Angiogenesis and Growth of Malignant Melanoma in Mice (2008)
  • Author(s): Koga M, Ikeda A, 他10名5番目
  • Journal Title: Biochem Biophys Res Comm 365 Pages: 279-284
  • Peer Reviewed
• [Presentation] レーザーマイクロダイセクション法を用いた心筋構成細胞の選択的遺伝子発現解析法 (2009)
  • Author(s): 安岡逸、池田理望, (他4 名2 番目)
  • Organizer: 日本心不全学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-10-31
• [Presentation] レーザーマイクロダイセクション法を用いた心筋構成細胞の選択的遺伝子発現解析法 (2009)
  • Author(s): 安岡逸、池田理望, 他4名2番目
  • Organizer: 第13回日本心不全学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-10-31
• [Presentation] Asymmeteric dimethylarginine induces endothelial dysfunction in renal failure mice (2009)
  • Author(s): Kajimoto H, Ikeda A, (他4 名4 番目)
  • Organizer: 日本循環器学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-03-21
• [Presentation] Selective collection and comprehensive gene expression analysis of each tissue component of the heart using laser microdissection method (2009)
  • Author(s): Ikeda A, (他5 名1番目)
  • Organizer: 日本循環器学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-03-21
• [Presentation] Selective collection and comprehensive gene expression analysis of each tissue component of the heart using lase microdissection method (2009)
  • Author(s): Ikeda A、他5名1番目
  • Organizer: 日本循環器学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-03-21
• [Presentation] Enhanced cardiac inflammation and myocardial fibrosis in ovariectomized (2009)
  • Author(s): Mori T, Ikeda A, (他7 名6 番目)
  • Organizer: pressure-overloaded rats -A possible mechanism of aggravation of diastolic dysfunction in the post-menopause- American Heart Association Scientific Meeting 2009
  • Place of Presentation: Orlando, Florida, USA
• [Presentation] レーザーマイクロダイセクション法を用いた心筋構成細胞の選択的遺伝子発現解析法の開発 (2008)
  • Author(s): 池田理望, (他4 名1番目)
  • Organizer: 日本高血圧学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2008-10-11
• [Presentation] Simvastatin attenuates large blood pressure variability-induced aggravation of cardiac hypertrophy, but not myocardial fibrosis, by inhibiting Ras-ERK and Rho A pathways in hypertensive rats (2008)
  • Author(s): Takayama N, Ikeda A, (他4 名8 番目)
  • Organizer: New cardioproteictive effects of statins- 62nd High Blood Pressure Research Conference 2008 and Scientific Sessions of the Council for High Blood Pressure Research
  • Place of Presentation: Atlanta, USA
• [Presentation] Role of Asymmetric Dimethylarginine in endothelial dysfunction in renal failure mice (2008)
  • Author(s): Kajimoto H, Ikeda A, (他3 名4 番目)
  • Organizer: 62nd High Blood Pressure Research Conference 2008 and Scientific Sessions of the Council for High Blood Pressure Research
  • Place of Presentation: Atlanta, USA
• [Presentation] Role of Asymmetric Dimethylarginine in endothelial dysfunction in renal failure mice (2008)
  • Author(s): Kajimoto H, Ikeda A、他3名4番目
  • Organizer: 62^<nd> High Blood Pressure Research Conference 2008 and Scientific Sessions of the Council for High Blood Pressure Research
  • Place of Presentation: Atlanta, USA
• [Presentation] Simvastatin attenuates large blood pressure variability-induced aggravation of cardiac hypertrophy, but not myocardial fibrosis, by inhibiting Ras-ERK and Rho A pathways in hypertensive rats.-New cardioproteictive effects of statins- (2008)
  • Author(s): Takayama N, Ikleda A、他4名8番目
  • Organizer: 62^<nd> High Blood Pressure Research Conference 2008 and Scientific Sessions of the Council for High Blood Pressure Research
  • Place of Presentation: Atlanta, USA