The study of pathogenesis during respiratory tract infection usingCCL1 gene targeted mouse

• Project/Area Number: 20790563
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Yamagata University
• Principal Investigator: INOUE Sumito Yamagata University, 医学部, 助教 (70466621)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: CCL1 / COPD / 呼吸器感染症

Research Abstract

We originally established novel gene targeted mice, which can strongly express theCCL1 only in the lung tissues under the control of surfactant protein C (SP-C) promoter. To date, we confirmed that significantly higher expression ofCCL1 in bronchial alveolar fluid (BALF) in CCL1 gene targeted mice, compared with control mice. To evaluate the role of CCL1 during the respiratory infection or pathogenesis of chronic obstructive pulmonary disease, bacterial infection or pulmonary emphysema will be induced in these mice.

Research Products

• [Journal Article] Azithromycin suppresses interleukin-12 p40 expression in lipopolysaccharide and interferon-gamma stimulated macrophages. (2009)
  • Author(s): Yamauchi K, Shibata Y, Kimura T, Abe S, Inoue S, et. al.
  • Journal Title: Int J Biol Sci. 23 Pages: 667-78
  • Peer Reviewed
• [Journal Article] Effect of antibiotic therapy on the inflammatory responses during streptococcal pneumonia in emphysematous mice. (2009)
  • Author(s): Inoue S, Nakamura H, Shibata Y, et. al.
  • Journal Title: EXCLI Journal 8 Pages: 50-7
  • Peer Reviewed
• [Journal Article] Gly80Ser Polymorphism of Phospholipase A2-IID Is Associated with Cytokine Inducibility in A549 Cells. (2009)
  • Author(s): Igarashi A, Shibata Y, Yamauchi K, Osaka D, Takabatake N, Abe S, Inoue S, et. al.
  • Journal Title: Respiration 78 Pages: 312-21
  • Peer Reviewed
• [Journal Article] A novel polymorphism in CDC6 is associated with the decline in lung function of ex-smokers in COPD. (2009)
  • Author(s): Takabatake N, Toriyama S, Igarashi A, Tokairin Y, Takeishi Y, Konta T, Inoue S, et. al.
  • Journal Title: Biochem Biophys Res Commun 17,381(4) Pages: 554-9
  • Peer Reviewed
• [Journal Article] COPDと遺伝子多型呼吸 (2009)
  • Author(s): 井上純人、柴田陽光、阿部修一
  • Journal Title: vol.28 Pages: 361-365
• [Journal Article] Azithromycin suppresses interleukin-12p40 expression in lipopolysaccharide and interferon-gamma stimulated macrophages (2009)
  • Author(s): Yamauchi K
  • Journal Title: Int J Biol Sci. 23 Pages: 667-78
  • Peer Reviewed
• [Journal Article] Effect of antibiotic therapy on the inflammatory responses during streptococcal pneumonia in emphysematous mice (2009)
  • Author(s): Inoue S
  • Journal Title: EXCLI Journal 8 Pages: 50-7
  • Peer Reviewed
• [Journal Article] Gly80Ser Polymorphism of Phospholipase A2-IID Is Associated with Cytokine Inducibility in A549 Cells (2009)
  • Author(s): Igarashi A
  • Journal Title: Respiration 78 Pages: 312-21
  • Peer Reviewed
• [Journal Article] Altered expression of antimicrobial molecules in cigarette smoke-exposed emphysematous mice lungs. (2008)
  • Author(s): Shibata Y, Abe S, Inoue S, et. al.
  • Journal Title: Respirology 13(7) Pages: 1061-5
  • Peer Reviewed
• [Journal Article] Altered expression of antimicrobial molecules in cigarette smoke-exposed emphysematous mice lungs (2008)
  • Author(s): Shibata Y, Abe S, Inoue S, Kubota I., et.al.
  • Journal Title: Respirology 13 Pages: 1061-5
  • Peer Reviewed
• [Journal Article] Enhanced immediate inflammatory response to Streptococcus pneumoniae in the lungs of mice with pulmonary emphysema (2008)
  • Author(s): Tokairin Y, Shibata Y, Inoue S, Kubota I, et.al.
  • Journal Title: Respirology 13 Pages: 324-32
  • Peer Reviewed
• [Presentation] Exercise tolerance in patients with chronic obstructive pulmonary disease is independent of resting pulmonary or cardiac functions. (2010)
  • Author(s): Hiroyuki Kishi, Sumito Inoue, et. al.
  • Organizer: American Thoracic Society
  • Place of Presentation: New Orleans, USA
• [Presentation] Cigarette smoking causes airflow limitation in Japanese healthy individuals: Takahata Study. (2010)
  • Author(s): Daisuke Osaka, Yoko Shibata, Shuichi Abe, Sumito Inoue, et. al.
  • Organizer: American Thoracic Society 2010
  • Place of Presentation: New Orleans, USA
• [Presentation] Decrease in the numbers of alveolar macrophage in dominant negative MafB transgenic mice. (2010)
  • Author(s): Michiko Sato, Yoko Shibata, Tomomi Kimura, Shuichi Abe, Sumito Inoue, et. al.
  • Organizer: American Thoracic Society 2010
  • Place of Presentation: New Orleans, USA