Detection of tumor-derived mutations in circulating DNA

• Project/Area Number: 20790565
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Kanazawa University
• Principal Investigator: KIMURA Hideharu Kanazawa University, 医学部, 講師 (40444202)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 癌 / 遺伝子変異 / 血中DNA

Research Abstract

Patients with EGFR mutations were enrolled in this study. The concentration of the DNA extracted was higher in the patients' serum than in their plasma. The rate of EGFR mutation detection was higher, in their DNA from plasma, and by the Scorpion ARMS method. The detection rate was higher when whole genome amplification was performed.A larger study and the establishment of a more sensitive assay is needed to achieve the purpose of this study.

Research Products

• [Journal Article] Eosinophilic pneumonia and thoracic metastases as an initial manifestaion of prostatic carcinoma. (2008)
  • Author(s): Ishiguro T, Kimura H, Araya T, et al.
  • Journal Title: Internal Med 47 Pages: 1419-1423
  • Peer Reviewed
• [Journal Article] Eosinophilic pneumonia and thoracic metastases as an initial manifestaion of prostatic carcinoma (2008)
  • Author(s): Ishiguro T, Kimura H, et.al.
  • Journal Title: Intern Med 47 Pages: 1419-1423
  • Peer Reviewed
• [Presentation] Prognostic and predictive impact of EGFR and K-ras mutation, and EGFR gene copy number in patients with advanced non-small cell lung cancer (NSCLC) who received first-line cytotoxic chemotherapy. (2009)
  • Author(s): Tambo Y, et al.
  • Organizer: ASCO Annual Meeting 2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-05-31
• [Presentation] Prognostic and predictive impact of EGFR and K-ras mutation, and EGFR gene copy number in patients with advanced non-small cell lung cancer(NSCLC)who received first-line cytotoxic chemotherapy (2009)
  • Author(s): Tambo Y
  • Organizer: ASCO Annual Meeting 2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-05-31
• [Presentation] Using whole genome amplification of plasma DNA can increase the success rate of tumor-derived mutation detection in patients with advanced non-small cell lung cancer(NSCLC). (2009)
  • Author(s): Kimura H
  • Organizer: CNAPS-VI Hong Kong
  • Place of Presentation: Hong Kong
• [Presentation] ら. EGFR遺伝子変異、遺伝子増幅と細胞傷害性化学療法の治療効果の関連性 (2009)
  • Author(s): 丹保裕一
  • Organizer: 第49回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
• [Presentation] Detection of micro-dissemination of cancer cells by testing for progastrin-releasing peptide expression in the peripheral blood of SCLC patients. (2009)
  • Author(s): Kimura H
  • Organizer: AACR Annual Meeting 2009
  • Place of Presentation: Denver
• [Presentation] Detection of micro-dissemination of cancer cells by testing for progastrin-releasing peptide expression in the peripheral blood of SCLC patients (2009)
  • Author(s): Kimura H
  • Organizer: AACR Annual Meeting 2009
  • Place of Presentation: Denver
• [Presentation] EGFR遺伝子変異、遺伝子増幅と細胞傷害性化学療法の治療効果の関連性 (2009)
  • Author(s): 丹保裕一
  • Organizer: 第49回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
• [Presentation] Using whole genome amplification of plasma DNA can increase the success rate of tumor-derived mutation detection in patients with advanced non-small cell lung cancer(NSCLC) (2009)
  • Author(s): Kimura H
  • Organizer: CNAPS-VI Hong Kong
  • Place of Presentation: Hong Kong
• [Presentation] 腫瘍代替サンプルを用いた癌由来遺伝子変異の検出 (2008)
  • Author(s): 木村英晴
  • Organizer: 第49回日本肺癌学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2008-11-13
• [Presentation] Suitable Sourse of DNA for a Bool-based test for EGFR Mutations. (2008)
  • Author(s): 木村英晴
  • Organizer: 第67回日本癌学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Suitable Sourse of DNA for a Bool-based test for EGFR Mutations (2008)
  • Author(s): 木村英晴
  • Organizer: 第67回日本癌学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] 腫瘍代替サンプルを用いた腫瘍由来変異遺伝子検出方法の確立、使用検体の検討 (2008)
  • Author(s): 木村英晴
  • Organizer: 第12回がん分子標的治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-27
• [Presentation] ゲフィチニブによる皮膚障害の発現を予測する因子としての血漿中サイトカイン測定の有用性 (2008)
  • Author(s): 木村英晴
  • Organizer: 第4回日本臨床プロテオーム研究会
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-10
• [Presentation] Suitable Sourse of DNA for a Bool-based test for EGFR Mutations. (2008)
  • Author(s): Kimura H, et al.
  • Organizer: AACR Annual Meeting 2008
  • Place of Presentation: San Diego
  • Year and Date: 2008-04-15
• [Presentation] Suitable Sourse of DNA for a Bool-based test for EGFR Mutations (2008)
  • Author(s): Kimura H, Sakai A, Kasahara K.
  • Organizer: AACR Annual Meeting 2008
  • Place of Presentation: San Diego
  • Year and Date: 2008-04-15
• [Presentation] Detection of EGFR mutations in accessible tissue that is a potential surrogate for tissue. (2008)
  • Author(s): Sakai A, et al.
  • Organizer: AACR Annual Meeting 2008
  • Place of Presentation: San Diego
  • Year and Date: 2008-04-14
• [Presentation] Detection of EGFR mutations in accessible tissue that is a potential surrogate for tissue (2008)
  • Author(s): Sakai A, Kimura H, et.al.
  • Organizer: AACR Annual Meeting 2008
  • Place of Presentation: San Diego
  • Year and Date: 2008-04-14
• [Book] 遺伝子変異検査. 「新臨床腫瘍学-がん薬物療法専門医のために-」(NPO法人日本臨床腫瘍学会編) (2009)
  • Author(s): 木村英晴
  • Publisher: 南行堂
• [Book] 「新臨床腫瘍学-がん薬物療法専門医のために-」(NPO法人日本臨床腫瘍学会編) (2009)
  • Author(s): 木村英晴
  • Publisher: 遺伝子変異検査