Investigation of the pathomechanisms of lung fibrosis by using bronchioalveolar epithelium-specific Pten deficient mice

• Project/Area Number: 20790573
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: University of Miyazaki
• Principal Investigator: YANAGI Shigehisa University of Miyazaki, 医学部, 助教 (60404422)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 肺線維症 / 分子標的治療

Research Abstract

Idiopathic pulmonary fibrosis is a chronic, lethal interstitial lung disease resulting from abnormal wound healing that involves epithelial mesenchymal interaction and epithelial-mesenchymal transition (EMT). To clarify the biological role of PTEN, in bronchioalveolar epithelium in the pathogenesis of lung fibrosis in vivo. KO mice showed high mortality compared with the control after bleomycin administration due to hypoxia caused by aberrant lung fibrosis. Lungs of KO mice exhibited increased accumulation of myofibroblasts and type I collagen. EMT significantly increased in KO mice. These results suggested that bronchioalveolar PTEN expression plays a critical role in preventing lung fibrosis by regulating EMT.

Research Products

• [Presentation] Function of Pten/PI3K/Akt Pathway in Bronchioalveolar Epithelium in the pathogenesis of Lung Fibrosis (2009)
  • Author(s): Shigehisa Yanagi
  • Organizer: European Respiratory Society Annual Congress
  • Place of Presentation: Vienna
  • Year and Date: 2009-09-16
• [Presentation] 細気管支肺胞上皮特異的Pten欠損マウスを用いたブレオマイシン肺線維症モデルの解析 (2009)
  • Author(s): 三好かほり、柳重久、今津善史、松元信弘、芦谷淳一、中里雅光
  • Organizer: 第49回日本呼吸器学会総会
  • Place of Presentation: 東京
• [Presentation] Function of PTEN/P13K/AKT pathway in bronchioalveolar epithelium in the pathogenesis of lung fibrosis (2009)
  • Author(s): Tsubouchi H, Yanagi S, Miyoshi K, Imazu Y, Arimura Y, Matsumoto N, Ashitani J, Kawahara K, Suzuki A, Nakazato M
  • Organizer: European Respiratory Society
  • Place of Presentation: Vienna, Austria
• [Remarks] 受賞2009年9月European Respiratory Society Annual Congress 2009 Cell & Molecular Biology Young Scientist Travel Award " Function of Pten/PI3K/Akt pathway in bronchioalveolar epithelium in the pathogenesis of lung fibrosis"