Loss-of Function screening for identification of new therapeutic target for malignant mesothelioma

• Project/Area Number: 20790582
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: SUDO Hitomi National Institute of Radiological Sciences, 分子イメージング研究センター, 准技術員 (10415416)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 中皮腫 / siRNA / 機能スクリーニング

Research Abstract

We conducted a large-scale functional screening of mesothelioma cells using a genome-wide small interfering RNA library. We determined that knockdown of 39 genes suppressed mesothelioma cell proliferation. In particular, COPA knockdown induced apoptosis and suppressed tumor growth in a mesothelioma mouse model. Therefore, COPA may have the potential of a therapeutic target of mesothelioma.

Research Products

• [Journal Article] Knockdown of COPA, Identified by Loss-of-Function Screen, Induces Apoptosis and Suppresses Tumor Growth in Mesothelioma Mouse Mode (2010)
  • Author(s): Hitomi Sudo, Atsushi B. Tsuji, Aya Sugyo, Masakazu Kohda, Chizuru Sogawa, Chisato Yoshida, Yoshi-nobu Harada, Okio Hino Tsuneo Saga
  • Journal Title: Genomics 95 Pages: 210-216
• [Journal Article] Knockdown of COPA, Identified by Loss-of-Function Screen, Induces Apoptosis and Suppresses Tumor Growth in Mesothelioma Mouse Model (2010)
  • Author(s): Hitomi Sudo
  • Journal Title: Genomics 95 Pages: 210-216
  • Peer Reviewed
• [Presentation] 中皮腫に対する治療薬のターゲット遺伝子のスクリーニング (2008)
  • Author(s): 須藤仁美、辻厚至、須堯綾、曽川千鶴、吉田千里、樋野興夫、佐賀恒夫
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] 中皮腫の増殖に関わる遺伝子の大規模スクリーニング (2008)
  • Author(s): 須藤仁美、辻厚至、曽川千鶴、原田良信、樋野興夫、佐賀恒夫
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28