Establishment of mouse model of human cryoglobulinemic glomerulonephritis
| Project/Area Number |
20790590
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kanazawa University |
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Principal Investigator |
ITO Kiyoaki Kanazawa University, 附属病院, 医員 (10467110)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | クリオグロブリン / 糸球体腎炎 / ハイブリドーマ / モデルマウス / リウマトイド因子 / モノクローナルRF / 管内性増殖性腎炎 / メサンギウム増殖性腎炎 |
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| Research Abstract |
The purpose of our study is to establish a mouse model of cryoglobulinemic (Cryo) glomerulonephritis (GN). We established the hybridoma cell line producing IgM-kappa cryoglobulin with rheumatoid factor activity (Cryo+IgM RF) from a patient with Cryo GN. We administered Cryo+IgM RF to wild type Balb/c mice. Urinary albumin increased. Pathological analysis of kidney tissue showed mild endocapillary proliferation, mild mesangial cell proliferation, and deposition of Cryo+IgM RF in the glomeruli. These results provide basic data for the establishment of Cryo GN although renal pathological changes are mild.
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Report
(3 results)
Research Products
(2 results)
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[Presentation] Somatic hypermutation and intraclonal diversity in monoclonal IgM rheumatoid factor in mixed cryoglobulinemia.2009
Author(s)
Kazunori Yamada, Mitsuhiro Kawano, Ryo Inoue, Hiroshi Fujii, Kiyoaki Ito, Ryoko Hamano, Yasushi Kakuchi, Masami Matsumura, Ichiro Koni, Akihiro Yachie, Masakazu Yamagishi.
Organizer
World Congress of Nephrology
Place of Presentation
Milan Convention Center, Milan, Italy
Related Report
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