Establishment of mouse model of human cryoglobulinemic glomerulonephritis

Research Project

Project/Area Number	20790590
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Kidney internal medicine
Research Institution	Kanazawa University
Principal Investigator	ITO Kiyoaki Kanazawa University, 附属病院, 医員 (10467110)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	クリオグロブリン / 糸球体腎炎 / ハイブリドーマ / モデルマウス / リウマトイド因子 / モノクローナルRF / 管内性増殖性腎炎 / メサンギウム増殖性腎炎
Research Abstract	The purpose of our study is to establish a mouse model of cryoglobulinemic (Cryo) glomerulonephritis (GN). We established the hybridoma cell line producing IgM-kappa cryoglobulin with rheumatoid factor activity (Cryo+IgM RF) from a patient with Cryo GN. We administered Cryo+IgM RF to wild type Balb/c mice. Urinary albumin increased. Pathological analysis of kidney tissue showed mild endocapillary proliferation, mild mesangial cell proliferation, and deposition of Cryo+IgM RF in the glomeruli. These results provide basic data for the establishment of Cryo GN although renal pathological changes are mild.