Comprehensive gene expression profiling analysis on diabetic nephropathy

• Project/Area Number: 20790592
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Nagoya University
• Principal Investigator: YOSHINARI Yasuda Nagoya University, 大学院・医学系研究科, 寄附講座准教授 (60432259)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 糖尿病性腎症 / DNAアレイ / バイオ・インフォマティクス / 糖尿病性腎 / cDNAアレイ / 網羅的遺伝子発現解析 / バイオインフォマティクス / NF-κB / interferon regulatory factor (IRF)

Research Abstract

A comprehensive gene expression analysis was performed by using mRNA extracted from human renal biopsy specimens after micro-disection, however signal sensitivity has still remained at the level of detection limit. In parallel, a bio-informatics analysis was conducted by using animal models of acute kidney injury. Comparison of the gene expression signatures of murine renal failure identified a shared transcriptome of 45 differentially regulated genes. 67% of these mRNAs were regulated in human renal disease and successfully segregated progressive DN from controls by clustering analysis.

Research Products

• [Journal Article] Modification of a new estimating equation for GFR, CKD-EPI equation for Japanese. (2010)
  • Author(s): Horio M, Imai E, Yasuda Y, Watanabe T, Matsuo S.
  • Journal Title: Am J Kid Dis. (in press)
• [Journal Article] Current status of estimated GFR (eGFR) equations for Asians and an approach to create a common eGFR equation. (2010)
  • Author(s): Matsuo S, Yasuda Y, Imai E, Horio M.
  • Journal Title: Nephrol. (in press)
• [Journal Article] Effects of oral cilostazol 100 mg BID on long-term patency after percutaneous transluminal angioplasty in patients with femoropopliteal disease undergoing hemodialysis: a retrospective chart review in Japanese patients. (2010)
  • Author(s): Ishii H, Kumada Y, Toriyama T, Aoyama T, Takahashi H, Tanaka M, Kamoi D, Kawamura Y, Yamada S, Hayashi M, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Clin Ther. 32(1) Pages: 24-33
• [Journal Article] Impact of renal function on coronary plaque composition. (2010)
  • Author(s): Miyagi M, Ishii H, Murakami R, Isobe S, Hayashi M, Amano T, Arai K, Yoshikawa D, Ohashi T, Uetani T, Yasuda Y, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Nephrol Dial Transplant. 25(1) Pages: 175-81
• [Journal Article] Prognostic values of C-reactive protein levels on clinical outcome after implantation of sirolimus-eluting stents in patients on hemodialysis. (2009)
  • Author(s): Ishii H, Toriyama T, Aoyama T, Takahashi H, Amano T, Hayashi M, Tanaka M, Kawamura Y, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Circ Cardiovasc Interv. 2(6) Pages: 513-8
• [Journal Article] Impact of microarousal associated with increased negative esophageal pressure in sleep-disordered breathing. (2009)
  • Author(s): Sukegawa M, Noda A, Yasuda Y, Nakata S, Sugiura T, Miyata S, Honda K, Hasegawa Y, Nakashima T, Koike Y.
  • Journal Title: Sleep Breath. (Epub ahead of print)
• [Journal Article] Collaborators developing the Japanese equation for estimated GFR. (2009)
  • Author(s): Matsuo S, Imai E, Horio M, Yasuda Y, Tomita K, Nitta K, Yamagata K, Tomino Y, Yokoyama H, Hishida A
  • Journal Title: Revised equations for estimated GFR from serum creatinine in Japan. Am J Kidney Dis. 53(6) Pages: 982-92
• [Journal Article] Aortic valvular calcification predicts restenosis after implantation of drug-eluting stents in patients on chronic haemodialysis (2009)
  • Author(s): Ishii H, Kumada Y, Toriyama T, Aoyama T, Takahashi H, Amano T, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Nephrol Dial Transplant. 24(5) Pages: 1562-7
• [Journal Article] on behalf of the collaborators for developing the Japanese equation for estimating GFR. (2009)
  • Author(s): Horio M, Imai E, Yasuda Y, Hishida A, Matsuo S
  • Journal Title: Simple sampling strategy for measuring inulin renal clearance. Clin Exp Nephrol. 13(1) Pages: 50-4
• [Journal Article] Revised Equations for Estimated GFR From Serum Creatinine in Japan (2009)
  • Author(s): Matsuo S
  • Journal Title: American journal of kidney diseases 53(6) Pages: 982-992
  • Peer Reviewed
• [Journal Article] Impact of microarousal associated with increased negative esophageal pr essure in sleep-disordered breathing. (2009)
  • Author(s): Sukegawa M
  • Journal Title: Sleep and breathing 13(4 Pages: 369-377
  • Peer Reviewed
• [Journal Article] Impact of renal function on coronary plaque composition. (2009)
  • Author(s): Miyagi M
  • Journal Title: Nephrology dialysis transplantation 25(1) Pages: 175-181
  • Peer Reviewed
• [Journal Article] Prognostic values of C-reactive protein levels on clinical outcome after implantation of sirolimus-eluting stents in patients on hemodialysis. (2009)
  • Author(s): Ishii H
  • Journal Title: Circ Cardiovasc Interv 2(6) Pages: 513-518
  • Peer Reviewed
• [Journal Article] Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import (2009)
  • Author(s): Ogawa Y
  • Journal Title: Mol Biol Cell 21(4 Pages: 630-638
  • Peer Reviewed
• [Journal Article] Aortic pressure augmentation as a marker of cardiovascular risk in obstructive sleep apnea syndrome. (2008)
  • Author(s): Noda A, Nakata S, Fukatsu H, Yasuda Y, Miyao E, Miyata S, Yasuma F, Murohara T, Yokota M, Koike Y.
  • Journal Title: Hypertens Res. 31(6) Pages: 1109-14
  • NAID: 10021936343
• [Journal Article] Cilostazol improves long-term patency after percutaneous transluminal angioplasty in hemodialysis patients with peripheral artery disease. (2008)
  • Author(s): Ishii H, Kumada Y, Toriyama T, Aoyama T, Takahashi H, Yamada S, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Clin J Am Soc Nephrol. 3(4) Pages: 1034-40
• [Journal Article] Sirolimus-eluting stents vs bare metal stents for coronary intervention in Japanese patients with renal failure on hemodialysis. (2008)
  • Author(s): Aoyama T, Ishii H, Toriyama T, Takahashi H, Kasuga H, Murakami R, Amano T, Uetani T, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Circ J. 72(1) Pages: 56-60
  • NAID: 110006533914
• [Journal Article] Sirolimus-eluting stents vs bare metal stents for coronary intervention in Japanese patients with renal failure on hemodialysis (2008)
  • Author(s): Aoyama T
  • Journal Title: Circulation journal 72(1) Pages: 56-60
  • Peer Reviewed
• [Journal Article] Cilostazol Improves Long-Term Patency after Percutaneous Transluminal Angioplasty in Hemodialysis Patients with Peripheral Artery Disease (2008)
  • Author(s): Ishii H
  • Journal Title: Clinical journal of the American Society of Nephrology 3(4) Pages: 1034-1040
  • Peer Reviewed
• [Journal Article] Aortic pressure augmentation as a marker of cardiovascular risk in obstructive sleep apnea syndrome (2008)
  • Author(s): Noda A
  • Journal Title: Hypertension Research 31(6) Pages: 1109-14
  • Peer Reviewed
• [Journal Article] Simple sampling strategy for measuring inulin renal clearance (2008)
  • Author(s): Horio M
  • Journal Title: Clinical and experimental nephrology (In press)
  • Peer Reviewed
• [Journal Article] Aortic valvular calcification predicts restenosis after implantation of drug-eluting stents in patients on chronic haemodialysis (2008)
  • Author(s): Ishii H
  • Journal Title: Nephrology dialysis transplantation (In press)
  • Peer Reviewed
• [Presentation] 保存期慢性腎不全(CRF)に全身性結節性痒疹を併発し、腹膜透析(PD)開始で著明に改善した一例 (2009)
  • Author(s): 冨田貴子
  • Organizer: 第54回日本透析医学会学術集会総会
  • Place of Presentation: 横浜
• [Presentation] NSAIDs使用患者におけるeGFR式による腎機能評価 (2009)
  • Author(s): 柴田佳菜子
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Presentation] The Age-Specific Reference Values of Estimated Glomerular Filtration Rate and Their Regional Variation in Japan (2009)
  • Author(s): Rieko Okada
  • Organizer: 41rd Annual Meeting of The American Society of Nephrology
  • Place of Presentation: San Diego, California, USA
• [Presentation] Morphological Renal Abnormalities by Ultrasound among Health Check Subjects : Clinical Significance of Silent Renal Stones or Cysts in Chronic Kidney Disease (2009)
  • Author(s): Kiyonari Kato
  • Organizer: 41rd Annual Meeting of The American, Society of Nephrology
  • Place of Presentation: San Diego, California, USA
• [Presentation] Revised Equations for Estimating Glomerular Filtration Rate from Serum Creatinine in Japan (2008)
  • Author(s): Seiichi Matsuo
  • Organizer: 41 rd Annual Meeting of The American Society of Nephrology
  • Place of Presentation: Philadelphia