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Derlin-1 overexpression ameliorates mutant SOD1-induced endoplasmic reticulum stress by reducing mutant SOD1 accumulation

Research Project

Project/Area Number 20790619
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionKumamoto University

Principal Investigator

YAMASHITA Satoshi  Kumamoto University, 大学院・生命科学研究部, 特任助教 (20457592)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords(A)神経分子病態学 / 神経科学 / 遺伝子治療 / 神経変性疾患 / 筋萎縮性側索硬化症 / 小胞体ストレス / アポトーシス / 小胞体関連分解 / アデノ随伴ウイルス
Research Abstract

Unfolded protein responses, including induction of stress sensor kinases, chaperones, and apoptotic mediators, are involved in the familial amyotrophic lateral sclerosis (ALS) model related to mutant Cu/Zn superoxide dismutase (SOD1) and sporadic ALS. We hypothesized that the endoplasmic reticulum-resident factor Derlin-1 plays a pivotal role in the regulation of misfolded proteins evoked by mutant SOD1. We show that Derlin-1 overexpression reduced mutant SOD1-induced cell toxicity and increased cell viability by suppressing the activation of the ER stress pathway factors : immunoglobulin-binding protein, activating transcription factor 6 p50, and C/EBP homologous protein. Interestingly, exogenous Derlin-1 resulted in a decrease in the amount of mutant SOD1, and a lesser decrease in that of wild-type SOD1, in transfected cells. Reduced SOD1 protein expression was observed in the microsomal fraction of wild-type and mutant SOD1 cells. Our results indicate that Derlin-1 regulates the turn over of SOD1 by promoting the proteasomal and autophagosomal degradation of SOD1 protein, but not by decreasing mutant SOD1 mRNA levels. Insights into the effects of Derlin-1 on mutant SOD1 may facilitate advancements in the treatment of motor neuron degeneration associated with ALS.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 2009 2008

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (12 results)

  • [Journal Article] Derlin-1overexpression ameliorates mutant SOD1-induced endoplasmic reticulum stress by reducing mutant SOD1 accumulation.2011

    • Author(s)
      Mori A, Yamashita S, et al.
    • Journal Title

      Neurochem Int 58

      Pages: 344-353

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Derlin-1 overexpression ameliorates mutant SOD1-induced endoplasmic reticulum stress by reducing mutant SOD1 accumulation.2011

    • Author(s)
      Akira Mori, et al.
    • Journal Title

      Neurochemistry International

      Volume: 58 Pages: 344-353

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amyotrophic lateral sclerosis in a patient with Kartagener syndrome.2010

    • Author(s)
      Yamashita S, et al.
    • Journal Title

      Amyotroph Lateral Scler 11

      Pages: 402-404

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] DJ-1 forms complexes with mutant SOD1 and ameliorates its toxicity.2010

    • Author(s)
      Yamashita S, et al.
    • Journal Title

      J Neurochem 113

      Pages: 860-870

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] DJ-1 forms complexes with mutant SOD1 and ameliorates its toxicity.2010

    • Author(s)
      Satoshi Yamashita, et al.
    • Journal Title

      Journal of neurochemistry

      Volume: 113 Pages: 860-870

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amyotrophic lateral sclerosis in a patient with Kartagener syndrome.2010

    • Author(s)
      Satoshi Yamashita, et al.
    • Journal Title

      Amyotrophic lateral sclerosis

      Volume: 11 Pages: 402-404

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flexor-dominant myopathic phenotype in patients with His46Arg substitution in the Cu/Zn superoxide dismutase gene.2009

    • Author(s)
      Yamashita S, et al.
    • Journal Title

      J Neurol Sci 281

      Pages: 6-10

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] DJ-1 forms complexes with mutant SOD1 and ameliorates its toxicity.2010

    • Author(s)
      Yamashita S, et al.
    • Organizer
      Society for Neuroscience 2010, 462.13
    • Place of Presentation
      San Diego, San Diego Convention Center, USA
    • Year and Date
      2010-11-15
    • Related Report
      2010 Final Research Report
  • [Presentation]2010

    • Author(s)
      Mori A, Yamashita S, et al.
    • Organizer
      Society for Neuroscience 2010, 462.11
    • Place of Presentation
      San Diego, San Diego Convention Center, USA
    • Year and Date
      2010-11-15
    • Related Report
      2010 Final Research Report
  • [Presentation] DJ-1 forms complexes with mutant SOD1 and ameliorates its toxicity.2010

    • Author(s)
      Satoshi Yamashita
    • Organizer
      Society for Neuroscience 2010
    • Place of Presentation
      Convention Center, San Diego, USA
    • Year and Date
      2010-11-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Derlin-1 overexpression ameliorates mutant SOD1-induced endoplasmic reticulum stress by reducing mutant SOD1 accumulation.2010

    • Author(s)
      Akira Mori
    • Organizer
      Society for Neuroscience 2010
    • Place of Presentation
      Convention Center, San Diego, USA
    • Year and Date
      2010-11-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] DJ-1 forms complexes with mutant SOD1 and ameliorates its toxicity.2010

    • Author(s)
      Yamashita S, et al.
    • Organizer
      Neuro 2010
    • Place of Presentation
      Kobe, Kobe Convention Center, Japan
    • Year and Date
      2010-09-02
    • Related Report
      2010 Final Research Report
  • [Presentation] DJ-1 forms complexes with mutant SOD1 and ameliorates its toxicity.2010

    • Author(s)
      Satoshi Yamashita
    • Organizer
      Neuro 2010
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] DJ-1は変異SOD1と相互作用し、神経毒性を軽減する2010

    • Author(s)
      山下賢
    • Organizer
      第51回日本神経学会総会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Year and Date
      2010-05-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] DJ-1は変異SOD1と相互作用し、神経毒性を軽減する2010

    • Author(s)
      山下賢, ら
    • Organizer
      第51回日本神経学会総会
    • Place of Presentation
      東京、東京国際フォーラム
    • Related Report
      2010 Final Research Report
  • [Presentation] 変異SOD1の小胞体内蓄積をターゲットとした家族性ALSの遺伝子治療開発2009

    • Author(s)
      森麗
    • Organizer
      第50回日本神経学会総会
    • Place of Presentation
      宮城県 仙台国際センター
    • Year and Date
      2009-05-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] DJ-1による変異SOD1毒性の軽減効果に関する検討2009

    • Author(s)
      山下賢, ら
    • Organizer
      第50回日本神経学会総会
    • Place of Presentation
      仙台、仙台国際センター
    • Related Report
      2010 Final Research Report
  • [Presentation] 変異SOD1の小胞体内蓄積をターゲットとした家族性ALSの遺伝子治療開発2008

    • Author(s)
      山下賢
    • Organizer
      第49回日本神経学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2008-05-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] 変異SOD1の小胞体内蓄積をターゲットとした家族性ALSの遺伝子治療開発2008

    • Author(s)
      山下賢, ら
    • Organizer
      第49回日本神経学会総会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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