| Project/Area Number |
20790624
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Juntendo University |
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Principal Investigator |
HATANO Taku Juntendo University, 医学部, 助教 (60338390)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | LRRK2 PARK8 / 家族性パーキンソン病 / 膜輸送 / Exocytosis / LRRK2 / iRNA / PARK8 / exocytosis |
|---|
| Research Abstract |
Leucin-rich repeat kinase 2 (LRRK2) is causative protein of PARK8-linked familial Parkinson's disease and associates with the membrane trafficking system. In this study, we have explored whether LRRK2 influences the exocytosis in cultured cells. We revealed that knocking down LRRK2 expression disrupted the exocytosis of growth hormone in PC12 cells, which are rat adrenal pheocromocytoma cell line. However, pathogenic LRRK2 mutants could not influence the exocytosis, compared to wild LRRK2. Therefore, LRRK2 might participate in the membrane trafficking system, but we need further investigations to understand whether pathogenic LRRK2 mutants may affect the functions of exocytosis.
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