The roles of proton-sensing receptors in bone remodeling

• Project/Area Number: 20790636
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: Gunma University
• Principal Investigator: MOGI Chihiro Gunma University, 生体調節研究所, 助教 (00375528)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 骨芽細胞 / 破骨細胞 / Gタンパク質共役型受容体 / プロトン / pH / 骨 / シグナル伝達

Research Abstract

It is well known that extracellular pH plays important roles in bone remodeling, but the molecular mechanisms are yet unidentified. To reveal the roles of novel four proton-sensing receptors (OGR1, GPR4, TDAG8, G2A), we investigated the functions of OGR1 in human osteoblasts and murine osteoclasts. Moreover, we examined the bone mineral density in femurs obtained from receptor-knockout mice.

Research Products

• [Journal Article] Mechanism and role of high density lipoprotein-induced activation of AMP-activated protein kinase in endothelial cells. (2010)
  • Author(s): Kimura T, Tomura H, Sato K, Ito M, Matsuoka I, Im DS, Kuwabara A, Mogi C, Itoh H, Kurose H, Murakami M, Okajima F
  • Journal Title: J Biol Chem. 285 Pages: 4387-97
  • Peer Reviewed
• [Journal Article] Mechanism and role of high density lipoprotein-induced activation of AMP-activated protein kinase in endothelial cells (2010)
  • Author(s): Kimura T, Tomura H, Sato K, Ito M, Matsuoka I, Im DS, Kuwabara A, Mogi C, Itoh H, Kurose H, Murakami M, Okajima F.
  • Journal Title: J Biol Chem. 285 Pages: 4387-4397
  • Peer Reviewed
• [Journal Article] Role of Rap1B and Tumor Suppressor PTEN in the Negative Regulation of Lysophosphatidic Acid-induced Migration by Isoproterenol in Glioma Cells. (2009)
  • Author(s): Malchinkhuu E, Sato K, Maehama T, Ishiuchi S, Yoshimoto Y, Mogi C, Kimura T, Kurose H, Tomura H, Okajima F
  • Journal Title: Mol Biol Cell. 20 Pages: 5156-65
  • Peer Reviewed
• [Journal Article] Inhibition of superoxide anion production by extracellular acidification in neutrophils. (2009)
  • Author(s): Murata N, §Mogi C, (§equally contributed) Tobo M, Nakakura T, Sato K, Tomura H, Okajima F
  • Journal Title: Cell Immunol 259 Pages: 21-26
  • Peer Reviewed
• [Journal Article] Involvement of Proton-Sensing TDAG8 in Extracellular Acidification-Induced Inhibition of Pro-Inflammatory Cytokine Production In Peritoneal Macrophages. (2009)
  • Author(s): Mogi C, Tobo M, Tomura H, Murata N, He XD, Sato K, Kimura T, Ishizuka T, Sasaki T, Sato T, Kihara Y, Ishii S, Harada A, Okajima F
  • Journal Title: J Immunol 182 Pages: 3243-3251
  • Peer Reviewed
• [Journal Article] LPA1 receptors mediate stimulation, whereas LPA2 receptors mediate inhibition, of migration of pancreatic cancer cells in response to lysophosphatidic acid and malignant ascites. (2009)
  • Author(s): Komachi M, Tomura H, Malchinkhuu E, Tobo M, Mogi C, Yamada T, Kimura T, Kuwabara A, Ohta H, Im DS, Kurose H, Takeyoshi I, Sato K, Okajima F
  • Journal Title: Carcinogenesis 30 Pages: 457-465
  • Peer Reviewed
• [Journal Article] LPA1 receptors mediate stimulation, whereas LPA2 receptors mediate inhibition, of migration of pancreatic cancer cells in response to lysophosphatidic acid and malignant ascites (2009)
  • Author(s): Komachi M, Tomura H, Malchinkhuu E, Tobo M, Mogi C, Yamada T, Kimura T, Kuwabara A, Ohta H, Im DS, Kurose H, Takeyoshi I, Sato K, Okajima F.
  • Journal Title: Carcinogenesis 30 Pages: 457-465
  • Peer Reviewed
• [Journal Article] Involvement of proton-sensing TDAG8 in extracellular acidification-induced inhibition of proinflammatory cytokine production in peritoneal macrophages (2009)
  • Author(s): Mogi C, Tobo M, Tomura H, Murata N, He XD, Sato K, Kimura T, Ishizuka T, Sasaki T, Sato T, Kihara Y, Ishii S, Harada A, Okajima F.
  • Journal Title: J Immunol. 182 Pages: 3243-3251
  • Peer Reviewed
• [Journal Article] Inhibition of superoxide anion production by extracellular acidification in neutrophils (2009)
  • Author(s): Murata N, Mogi C, Tobo M, Nakakura T, Sato K, Tomura H, Okajima F.
  • Journal Title: Cell Immunol. 259 Pages: 21-26
  • Peer Reviewed
• [Journal Article] Signaling pathways involved in DNA synthesis and migration in response to lysophosphatidic acid and low-density lipoprotein in coronary artery smooth muscle cells (2009)
  • Author(s): Komachi M, Damirin A, Malchinkhuu E, Mogi C, Tobo M, Ohta H, Sato K, Tomura H, Okajima F.
  • Journal Title: Vascul Pharmacol. 50 Pages: 178-184
  • Peer Reviewed
• [Journal Article] Role of Rap1B and tumor suppressor PTEN in the negative regulation of lysophosphatidic acid--induced migration by isoproterenol in glioma cells (2009)
  • Author(s): Malchinkhuu E, Sato K, Maehama T, Ishiuchi S, Yoshimoto Y, Mogi C, Kimura T, Kurose H, Tomura H, Okajima F.
  • Journal Title: Mol Biol Cell. 20 Pages: 5156-5165
  • Peer Reviewed
• [Journal Article] Involvement of proton-sensing TDAG8 in extracellular acidification-induced inhibition of proinflammatory cytokine production in peritoneal macrophages (2009)
  • Author(s): Mogi C, Tobo M, Tomura H, Murata N, He XD, Sato K, Kimura T, Ishizuka T, Sasaki T, Sato T, Kihara Y, Ishii S, Harada A, Okajima F
  • Journal Title: J Immunol 182 Pages: 3243-3251
  • Peer Reviewed
• [Journal Article] Induction of Scavenger Receptor Class B Type I is Critical for Simvastatin Enhancement of High-Density Lipoprotein-induced Anti-Inflammatory Actions in Endothelial Cells. (2008)
  • Author(s): Kimura T, §Mogi C, (§equally contributed) Tomura H, Kuwabara A, Im DS, Sato K, Kurose H, Murakami M, Okajima F
  • Journal Title: J Immunol 181 Pages: 7332-7340
  • Peer Reviewed
• [Journal Article] Cyclooxygenase-2 expression and prostaglandin E2 production in response to acidic pH through OGR1 in a human osteoblastic cell line. (2008)
  • Author(s): Tomura H, Wang JQ, Liu JP, Komachi M, Damirin A, Mogi C, Tobo M, Mochi H, Tamoto K, Im DS, Sato K, Okajima F.
  • Journal Title: J Bone Miner Res. 23 Pages: 1129-1139
  • Peer Reviewed
• [Journal Article] Cyclooxygenase-2 expression and prostaglandin E2 production in. response to acidic pH through OGR1 in a human osteoblastis cell line (2008)
  • Author(s): Tomura H, Wang JQ, Liu JP, Komachi M, Damirin A, Mogi C, Tobo M, Noch i H, Tamoto K, Im DS, Sato K, and Okajima F
  • Journal Title: J Bone Miner Res 23 Pages: 1129-1139
  • Peer Reviewed
• [Journal Article] Induction of scavenger receptor class B type I is critical for simvastatin enhancement of high-density lipoprotein-induced anti-inflammatory actions in endothelial cells (2008)
  • Author(s): Kimura T, Mogi C, Tomura H, Kuwabara A, Im DS, Sato K, Kurose H, Murakami M, Okajima F
  • Journal Title: J Immunol 181 Pages: 7332-7340
  • Peer Reviewed
• [Journal Article] LPA1 receptors mediate stimulation, whereas LPA2 receptors mediate inhibition, of migration of pancreatic cancer cells in response to lysophosphatidic acid and malignant ascites (2008)
  • Author(s): Komachi M, Tomura H, Malchinkhuu E, Tobo M, Mogi C, Yamada T, Kimura T, Kuwabara A, Ohta H, Im DS, Kurose H, Takeyoshi I, Sato K, Okanma F
  • Journal Title: Carcinogenesis 30 Pages: 457-465
  • Peer Reviewed
• [Presentation] ヒト大動脈平滑筋細胞における細胞外プロトン/OGR1受容体を介したシクロゲナーゼ-2の発現のリゾホスファチジン酸による増加 (2009)
  • Author(s): 戸村秀明, 劉進朋, 茂木千尋, 小町麻由美, 当房雅之, 中倉敬, 佐藤幸市, 岡島史和
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] マウスマクロファージの酸性下での炎症性サイトカインの産生低下におけるTDAG8の関与 (2009)
  • Author(s): 戸村秀明, 茂木千尋, 当房雅之, 中倉敬, 岡島史和
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
• [Presentation] ヒト大動脈血管平滑筋細胞のCOX-2発現/プロスタサイクリン産生応答におけるプロトン感知性GPCRとLPA受容体のクロストーク (2009)
  • Author(s): 劉進朋, 戸村秀明, 茂木千尋, 小町麻由美, 当房雅之, 佐藤幸市, 岡島史和
  • Organizer: 第51回日本脂質生化学会
  • Place of Presentation: 名古屋
• [Presentation] 細胞外プロトンをリガンドとするGタンパク質共役受容体の骨リモデリングにおける役割 (2009)
  • Author(s): 茂木千尋, 岡島史和
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 前橋
• [Presentation] 細胞外プロトンをリガンドとするGタンパク質共役受容体の骨リモデリングにおける役割 (2009)
  • Author(s): 茂木千尋、岡島史和
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬県民会館(前橋)
• [Presentation] ヒト大動脈血管平滑筋細胞のCOX-2発現/プロスタサイクリン産生応答におけるプロトン感知性GPCRとLPA受容体のクロストーク (2009)
  • Author(s): 劉進朋、戸村秀明、茂木千尋、小町麻由美、当房雅之、佐藤幸市、岡島史和
  • Organizer: 第51回日本脂質生化学会
  • Place of Presentation: ウィルあいち(愛知県女性総合センター:名古屋)
• [Presentation] ヒト大動脈平滑筋細胞における細胞外プロトン/OGR1受容体を介したシクロゲナーゼ-2の発現のリゾホスファチジン酸による増加 (2009)
  • Author(s): 戸村秀明、劉進朋、茂木千尋、小町麻由美、当房雅之、中倉敬、佐藤幸市、岡島史和
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] マウスマクロファージの酸性下での炎症性サイトカインの産生低下におけるTDAG8の関与 (2009)
  • Author(s): 戸村秀明、茂木千尋、当房雅之、中倉敬、岡島史和
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡グランシップ
• [Presentation] Role of TDAG8 in Extracellular Acidification-induced Inhibition of Pro-inflammatory Cytokine Production in Peritoneal Macrophages (2008)
  • Author(s): Chihiro Mogi, Masayuki Tobo, Hideaki Tomura, Naoya Murata, Xiao-dong He, Koichi Sato, Akihiro Harada, Fumikazu Okajima
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 細胞外pHの低下はOGR1受容体を介してヒト大動脈平滑筋細胞におけるシクロゲナーゼ-2の発現誘導とプロスタサイクリン産生の増加を引き起こす (2008)
  • Author(s): 戸村秀明, 一文字功, 石塚全, 小町麻由美, 当房雅之, 佐藤幸市, 茂木千尋, 森昌朋, 岡島史和
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 細胞外pHの低下はOGR1受容体を介してヒト大動脈平滑筋細胞におけるシクロゲナーゼ2の発現誘導とプロスタサイクリン産生の増加を引き起こす (2008)
  • Author(s): 劉進朋, 戸村秀明, 小町麻由美, 当房雅之, 佐藤幸市, 茂木千尋, 岡島史和
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Role of TDAG8 in Extracellular Acidification-induced Inhibition of Pro-inflammatory Cytokine Production in Peritoneal Macrophages (2008)
  • Author(s): Chihiro Mogi, Masayuki Tobo, Hideaki Tomura, Naoya Murata, Xiao-dong He, Koichi Sato, Akihiro Harada, and Fumikazu Okajima
  • Organizer: 第31回日本分子生物学瓜年酉、第81回日生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
• [Presentation] 細胞外pHの低下はOGR1受容体を介してヒト大動脈平滑筋細胞におけるシクロゲナーゼ-2の発現誘導とプロスタサイクリン産生の増加を引き起こす (2008)
  • Author(s): 戸村秀明、一文字功、石塚全、小町麻由美、当房雅之、佐藤幸市、茂木千尋、森昌朋、岡島史和
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
• [Presentation] 細胞外pHの低下はOGR1受容体を介してヒト大動脈平滑筋細胞におけるシクロゲナーゼ-2の発現誘導とプロスタサイクリン産生の増加を引き起こす (2008)
  • Author(s): 劉進朋、戸村秀明、小町麻由美、当房雅之、佐藤幸市、茂木千尋、岡島史和
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ボートアイランド(神戸)
• [Remarks]
  • URL: http://www.imcr.gunma-u.ac.jp/lab/sigtra/
• [Remarks]
  • URL: http://www.imcr.gunma-u.ac.jp/lab/sigtra/
• [Remarks]
  • URL: http://imcr.showa.gunma-u.ac.jp/lab/signal/index.html