Clarification of the role of annexin on glucose transport

• Project/Area Number: 20790638
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: The University of Tokyo
• Principal Investigator: FUJISHIRO Midori The University of Tokyo, 医学部附属病院, 助教 (50420211)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: エネルギー / 糖質代謝異常 / 糖尿病 / シグナル伝達 / 細胞・組織 / 蛋白質 / インスリン抵抗性

Research Abstract

Recently we have discovered a novel Akt binding protein, annexin, which is characterized by Ca2+-dependent interactions with cellular membranes. First we produced cDNA encoding human annexin by PCR with primers corresponding to sequences already reported, using the cDNA library from HEK293 cells and generated recombinant adenovirus to express human annexin. We confirmed the association of annexin with Akt using adenovirus system and then made GST-annexin fusion protein to generate the antibody specific against annexin by immunizing rabbits with this antigen. Fragments of both Akt and annexin were designed by PCR using full-length cDNAs as templates to generate GST-fused proteins.

Research Products

• [Journal Article] Macrophage foam cell formation is augmented in serum from patients with diabetic angiopathy. (2010)
  • Author(s): Cui X, Kushiyama A, Yoneda M, Nakatsu Y, Guo Y, Zhang J, Ono H, Kanna M, Sakoda H, Ono H, Kikuchi T, Fujishiro M, Shiomi M, Kamata H, Kurihara H, Kikuchi M, Kawazu S, Nishimura F, Asano T.
  • Journal Title: Diabetes Res Clin Pract. 87(1) Pages: 57-63
  • Peer Reviewed
• [Journal Article] Macrophage foam cell formation is augmented in serum from patients with diabetic angiopathy (2009)
  • Author(s): Cui X, Kushiyama A, Yoneda M, Fujishiro M, et al.
  • Journal Title: Diabetes Res Clin Pract. 87 Pages: 57-63
  • Peer Reviewed
• [Journal Article] Long-term forskolin stimulation induces AMPK activation and thereby enhances tight junction formation in human placental trophoblast BeWo cells. (2008)
  • Author(s): Egawa M, Kamata H, Kushiyama A, Sakoda H, Fujishiro M, Horike N, Yoneda M, Nakatsu Y, Ying G, Jun Z, Tsuchiya Y, Takata K, Kurihara H, Asano T.
  • Journal Title: Placenta 29(12) Pages: 1003-8
  • Peer Reviewed
• [Journal Article] AMP-activated protein kinase activation increases phosphorylation of glycogen synthase kinase 3beta and thereby reduces cAMP-responsive element transcriptional activity and phosphoenolpyruvate carboxykinase C gene expression in the liver. (2008)
  • Author(s): Horike N, Sakoda H, Kushiyama A, Ono H, Fujishiro M, Kamata H, Nishiyama K, Uchijima Y, Kurihara Y, Kurihara H, Asano T
  • Journal Title: J Biol Chem. 283(49) Pages: 33902-10
  • Peer Reviewed
• [Journal Article] Hepatic overexpression of a dominant negative form of raptor enhances Akt phosphorylation and restores insulin sensitivity in K/KAy mice. (2008)
  • Author(s): Koketsu Y, Sakoda H, Fujishiro M, Kushiyama A, Fukushima Y, Ono H, Anai M, Kikuchi T, Fukuda T, Kamata H, Horike N, Uchijima Y, Kurihara H, Asano T.
  • Journal Title: Am J Physiol Endocrinol Metab. 294(4) Pages: 719-25
  • Peer Reviewed
• [Journal Article] Regulation of gut-derived resistin-like molecule beta expression by nutrients. (2008)
  • Author(s): Fujio J, Kushiyama A, Sakoda H, Fujishiro M, Ogihara T, Fukushima Y, Anai M, Horike N, Kamata H, Uchijima Y, Kurihara H, Asano T
  • Journal Title: Diabetes Res Clin Pract. 79(1) Pages: 2-10
  • Peer Reviewed
• [Journal Article] Hepatic overexpression of a dominant negative form of raptor enhances Akt phosphorylation and restores insulin sensitivity in K/KAy mice (2008)
  • Author(s): Koketsu Y, Sakoda H, Fujishiro M, et.al.
  • Journal Title: Am J Physiol Endocrinol Metab 294
  • Peer Reviewed
• [Journal Article] AMP-ctivated protein kinase activation increases phosphorylation of glycogen synthase kinase 3beta and thereby reduces cAMP-responsive element transcriptional activity and phosphoenolpyruvate carboxykinase C gene expression in the liver (2008)
  • Author(s): Horike N, Sakoda H, Kushiyama A, Ono H, Fujishiro M, et.al.
  • Journal Title: J Biol Chem 223 Pages: 33902-10
  • Peer Reviewed
• [Journal Article] Long-term forskolin stimulation induces AMPK activation and thereby enhances tight junction formation in human placental trophoblast BeWo cells (2008)
  • Author(s): Egawa M, Kamata H, Kushiyama A, Sakoda H, Fujishiro M, et.al.
  • Journal Title: Placenta 29 Pages: 1003-8
  • Peer Reviewed
• [Presentation] 長時間高グルコース刺激による肝細胞内インスリン受容体蛋白発現調節および活性調節機構の解明 (2009)
  • Author(s): 藤城緑
  • Organizer: 第52回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-05-23