Role of Rho-associated kinase 2 in metabolic syndrome

• Project/Area Number: 20790646
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: Kyoto University
• Principal Investigator: NOGUCHI Michio Kyoto University, 医学研究科, 特定研究員 (00432394)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ROCK2 / メタボリックシンドローム / 脂肪細胞分化 / ROCK / 糖代謝

Research Abstract

We demonstrate that inhibition of ROCK2, not ROCK1, enhances adipogenesis accompanied by the upregulation of adipogenic transcription factors. Augmentation of insulin signaling may contribute to the enhancement of adipogenesis. Furtheremore, we focus on a cofactor as a novel mechanism associated with this pathway. ROCK2 heterozygous mice did not exhibit significant differences in metabolic parameters on standard chow and high fat diet compared with wild-type mice. Further studies will be needed.

Research Products

• [Journal Article] Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells (2009)
  • Author(s): D. Taura*, M. Noguchi*(* equally contributed), M. Sone, K. Hosoda, E. Mori, Y. Okada, K. Takahashi, K. Homma. N. Oyamada, M. Inuzuka, T. Sonoyama, K. Ebihara, N. Tamura, H. Itoh, H. Suemori, N. Nakatsuji, H. Okano, S. Yamanaka, K. Nakao
  • Journal Title: FEBS Lett. 583(6) Pages: 1029-1033
  • Peer Reviewed
• [Journal Article] Glucocorticoid reamplification within cells intensifies NF{kappa}B andMAPK signaling and reinforces inflammation in activated preadipocytes (2009)
  • Author(s): T. Ishii-Yonemoto, H. Masuzaki, S. Yasue, S. Okada, C. Kozuka, T. Tanaka, M. Noguchi, T. Tomita, J. Fujikura, Y. Yamamoto, K. Ebihara, K. Hosoda, K. Nakao
  • Journal Title: Am J Physiol Endocrinol Metab 298 Pages: 930-940
  • Peer Reviewed
• [Journal Article] Adipogenic differentiation of human induced pluripotent stem cells : comparison with that of human embryonic stem cells (2009)
  • Author(s): D.Taura^*, M.Noguchi^*(^* equally contributed), M.Sone, K.Hosoda, E.Mori, Y.Okada, K.Takahashi, K.Homma.N.Oyamada, M.Inuzuka, T.Sonoyama, K.Ebihara, N.Tamura, H.Itoh, H.Suemori, N.Nakatsuji, H.Okano, S.Yamanaka, K.Nakao
  • Journal Title: FEBS Lett. 583 Pages: 1029-1033
  • Peer Reviewed
• [Journal Article] Glucocorticoid reamplification within cells intensifies NF{kappa}B and MAPK signaling and reinforces inflammation in activated preadipocytes (2009)
  • Author(s): T.Ishii-Yonemoto, H.Masuzaki, S.Yasue, S.Okada, C.Kozuka, T.Tanaka, M.Nouchi, T.Tomita, J.Fujikura, Y.Yamamoto, K.Ebihara, K.Hosoda, K.Nakao
  • Journal Title: Am J Physiol Endocrinol Metab. 298 Pages: 930-940
  • Peer Reviewed
• [Journal Article] Adipose tissue-specific dysregulation of angiotensinogen by oxidative stress in obesity (2009)
  • Author(s): S.Okada, C.Kozuka, H.Masuzaki, S.Yasue, T.Ishii-Yonemoto, T.Tanaka, Y.Yamamoto, M.Noguchi, T.Kusakabe, T.Tomita, J.Fujikura, K.Ebihara, K.Hosoda, H.Sakaue, H.Kobori, M.Ham, Y.S.Lee, J.B.Kim, Y.Saito, K.Nakao
  • Journal Title: Metabolism
  • Peer Reviewed
• [Journal Article] Adipogenic differentiation of human induced pluripotent stem cells : comparison with that of human embryonic stem cells (2009)
  • Author(s): D. Taura, M. Noguchi, et.al.
  • Journal Title: FEBS Lett 583(6) Pages: 1029-33
  • Peer Reviewed
• [Journal Article] Index of the systemic balance of end products of glucocorticoid metabolism in fresh urine from humans. Its potential usefulness in the evaluation of obesity-related diseases (2009)
  • Author(s): N. Kobayashi, M. Noguchi, et.al.
  • Journal Title: Obesity Research and Clinical Practice
  • Peer Reviewed
• [Journal Article] Genetic and pharmacological inhibition of Rho-associated kinase II enhances adipogenesis. (2007)
  • Author(s): M. Noguchi, K. Hosoda, J. Fujikura, M. Fujimoto, H. Iwakura, T. Tomita, T. Ishii, N. Arai, M. Hirata, K. Ebihara, H. Masuzaki, H. Itoh, S. Narumiya, K. Nakao.
  • Journal Title: J Biol Chem. 282 Pages: 29574-29583
  • Peer Reviewed
• [Presentation] Role of Rho-associated kinase II in adipogenesis (2010)
  • Author(s): 野口倫生
  • Organizer: 14th International Congress of Endocrinology
  • Place of Presentation: 京都国際会館
  • Year and Date: 2010-03-28
• [Presentation] 脂肪細胞分化におけるRho-ROCK pathwayの意義 (2008)
  • Author(s): 野口倫生, 細田公則, 藤倉純二, 冨田努, 小鳥真司, 森栄作, 平田雅一, 海老原健, 益崎裕章, 中尾一和
  • Organizer: 第29回日本肥満学会
  • Place of Presentation: 大分
  • Year and Date: 2008-10-17
• [Presentation] 脂肪細胞分化、糖代謝におけるROCK-IIの意義 (2008)
  • Author(s): 野口倫生, 細田公則, 藤倉純二, 冨田努, 小鳥真司, 森栄作, 泰江慎太郎, 石井崇子, 岡田定規, 益崎裕章, 中尾一和
  • Organizer: 第51回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-24
• [Presentation] 脂肪細胞分化および糖代謝におけるROCK-IIの意義 (2008)
  • Author(s): 野口倫生, 細田公則, 藤倉純二, 冨田努, 小鳥真司, 森栄作, 泰江慎太郎, 石井崇子, 岡田定規, 海老原健, 平田雅一, 益崎裕章, 中尾一和
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森
  • Year and Date: 2008-05-16
• [Book] 日本肥満学会誌「肥満研究」, 脂肪細胞分化におけるRho-ROCK pathwayの意義 (2009)
  • Author(s): 野口倫生、細田公則
  • Total Pages: 4
  • Publisher: 日本肥満学会
• [Book] 分子細胞治療特集細胞骨格制御と疾患, メタボリックシンドロームと細胞骨格 (2009)
  • Author(s): 野口倫生、細田公則、中尾一和
  • Total Pages: 6
  • Publisher: 先端医学社
• [Book] 日本肥満学会誌「肥満研究」 (2009)
  • Author(s): 野口倫生、細田公則、中尾一和
  • Total Pages: 4
  • Publisher: 日本肥満学会
• [Book] 分子細胞治療 特集 細胞骨格制御と疾患 (2009)
  • Author(s): 野口倫生、細田公則、中尾一和
  • Total Pages: 6
  • Publisher: 先端医学社
• [Book] 日本肥満学会誌「肥満研究」 (2009)
  • Author(s): 野口倫生, 細田公則, 中尾一和
  • Total Pages: 4
  • Publisher: 日本肥満学会