| Project/Area Number |
20790652
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
KATSUMOTO Keiichi Kumamoto University, 発生医学研究所・COE リサーチ, アソシエイト (40398227)
|
|---|
| Research Collaborator |
KUME Shoen 熊本大学, 発生医学研究所, 教授 (70347011)
FUKUDA Kimiko 首都大学, 東京・理工学研究所, 准教授 (40285094)
SHIMAMURA Kenji 熊本大学, 生医学研究所, 教授 (70301140)
YASUGI Sadao 京都産業大学, 総合生命科学部, 教授 (70011591)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | 膵臓 / DiI 結晶 / 細胞運命予定地図 / 移植実験 / insulin / ニワトリ胚 / 内胚葉 / Pdx1 / ニワトリ / DiI / インスリン / アミラーゼ / β細胞 / 中胚葉 |
|---|
| Research Abstract |
To study the developmental origin of the pancreas we used DiI crystals to mark regions of the early chick endoderm: this allowed correlations to be established between specific endoderm sites and the positions of their descendants. Endodermal precursor cells for the stomach, pancreas and intestine were found to segregate immediately after completion of gastrulation. Transplantation experiments showed that region-specific endodermal fates are determined sequentially in the order stomach, intestine, and then pancreas. Non-pancreatic endoderm transplanted to the stomach region generated ectopic pancreas expressing both insulin and glucagon. These results imply that a pancreas-inducing signal is emitted from somitic mesoderm underlying the pre-pancreatic region, and this extends rostrally beyond the stomach endoderm region at the early somite stage. Transplantation experiments revealed that the endoderm responding to these pancreatic-inducing signals lies within the pre-pancreatic region and extends caudally beyond the region of the intestinal endoderm. The results indicate that pancreatic fate is determined in the area of overlap between these two regions.
|
