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Analysis of the pancreas regionalization factor in the pre-pancreas region.

Research Project

Project/Area Number 20790652
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Metabolomics
Research InstitutionKumamoto University

Principal Investigator

KATSUMOTO Keiichi  Kumamoto University, 発生医学研究所・COE リサーチ, アソシエイト (40398227)

Research Collaborator KUME Shoen  熊本大学, 発生医学研究所, 教授 (70347011)
FUKUDA Kimiko  首都大学, 東京・理工学研究所, 准教授 (40285094)
SHIMAMURA Kenji  熊本大学, 生医学研究所, 教授 (70301140)
YASUGI Sadao  京都産業大学, 総合生命科学部, 教授 (70011591)
Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords膵臓 / DiI 結晶 / 細胞運命予定地図 / 移植実験 / insulin / ニワトリ胚 / 内胚葉 / Pdx1 / ニワトリ / DiI / インスリン / アミラーゼ / β細胞 / 中胚葉
Research Abstract

To study the developmental origin of the pancreas we used DiI crystals to mark regions of the early chick endoderm: this allowed correlations to be established between specific endoderm sites and the positions of their descendants. Endodermal precursor cells for the stomach, pancreas and intestine were found to segregate immediately after completion of gastrulation. Transplantation experiments showed that region-specific endodermal fates are determined sequentially in the order stomach, intestine, and then pancreas. Non-pancreatic endoderm transplanted to the stomach region generated ectopic pancreas expressing both insulin and glucagon. These results imply that a pancreas-inducing signal is emitted from somitic mesoderm underlying the pre-pancreatic region, and this extends rostrally beyond the stomach endoderm region at the early somite stage. Transplantation experiments revealed that the endoderm responding to these pancreatic-inducing signals lies within the pre-pancreatic region and extends caudally beyond the region of the intestinal endoderm. The results indicate that pancreatic fate is determined in the area of overlap between these two regions.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (15 results)

All 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Embryonic and adult stem cell syst ems in mammals: Ontology and regulation2010

    • Author(s)
      Keiichi Katsumoto, Nobuaki Shiraki, Rika Miki, Shoen Kume
    • Journal Title

      Development, Growth&Differentiation 52

      Pages: 115-129

    • NAID

      10027563368

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryonic and adult stem cell systems in mammals : Ontology and regulation2010

    • Author(s)
      Keiichi Katsumoto
    • Journal Title

      Development, Growth & Differentiation 52

      Pages: 115-129

    • NAID

      10027563368

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conserved origin of the ventral pancreas in chicken.2009

    • Author(s)
      Kumi Matsuura*, Keiichi Katsumoto*, Kimiko Fukuda, Kazuhiko Kume, Shoen Kume(* equal contribution)
    • Journal Title

      Mechanisms of Development 126(10)

      Pages: 817-827

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Origin of pancreatic precursors in the chick embryo and the mechanism of endoderm regionalization.2009

    • Author(s)
      Keiichi Katsumoto, Kimiko Fukuda, Wataru Kimura, Kenji Shimamura, Sadao Yasugi, Shoen Kume
    • Journal Title

      Mechanisms of Development 126(7)

      Pages: 539-551

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conserved origin of the ventral pancreas in chicken2009

    • Author(s)
      Kumi Matsuura
    • Journal Title

      Mechanisms of Development 126

      Pages: 817-827

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Origin of pancreatic precursors in the chick embryo and the mechanism of endoderm regionalization2009

    • Author(s)
      Keiichi Katsumoto
    • Journal Title

      Mechanisms of Development (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] ニワトリ胚における背側、腹側膵臓形成メカニズムの解析2009

    • Author(s)
      勝本恵一松浦公美福田公子粂昭苑
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] The "sliding" between the endoderm and the mesoderm layer in the early embryo is a significant event for pancreas development2009

    • Author(s)
      Keiichi Katsumoto, Kimiko Fukuda, Wataru Kimura, Kenji Shimamura, Sadao Yasugi, Shoen Kume
    • Organizer
      International Society for Stem Cell Research
    • Place of Presentation
      Barcelona (Spain)
    • Year and Date
      2009-07-09
    • Related Report
      2009 Final Research Report
  • [Presentation] The "sliding" between the endoderm and the mesoderm layer in the early embryo is a significant event for pancreas development.2009

    • Author(s)
      Keiichi Katsumoto
    • Organizer
      International Society for Stem Cell Research
    • Place of Presentation
      Barcelona (Spain)
    • Year and Date
      2009-07-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] ニワトリ胚における膵臓前駆細胞の起源と初期内胚葉領域化メカニズムについての解析2009

    • Author(s)
      勝本恵一福田公子木村航嶋村健児八杉貞雄粂昭苑
    • Organizer
      日本発生生物学会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Year and Date
      2009-05-30
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 初期膵臓領域化は、内胚葉と中胚葉のズレにより生じる2008

    • Author(s)
      勝本恵一
    • Organizer
      日本発生生物学会サマースクール
    • Place of Presentation
      乗鞍(長野)
    • Year and Date
      2008-08-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] Analyses of the mechanism of dorsal pancreas specification in the early chick endoderm2008

    • Author(s)
      Keiichi Katsumoto
    • Organizer
      41st Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Tokushima, Japan
    • Year and Date
      2008-03-29
    • Related Report
      2008 Annual Research Report
  • [Book] Stem Cell Therapy(The potential of ES cells and tissue stem cells in the regenerative medicine of type I diabetes)2010

    • Author(s)
      Nobuaki Shiraki, Miki Rika, Keiichi Katsumoto, Shoen Kume
    • Publisher
      Transworld Research(in press)
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.imeg.kumamoto-u.ac.jp/newpress/np36.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.imeg.kumamoto-u.ac.jp/newpress/np31.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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