| Project/Area Number |
20790656
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Metabolomics
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
OKAMOTO Shiki National Institute for Physiological Sciences, 発達生理学研究系, 助教 (40342919)
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|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
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| Keywords | 脳・神経 / ウィルス / メタボリックシンドローム / AMPキナーゼ |
|---|
| Research Abstract |
Hypothalamic AMP-kinase regulates feeding behavior in response to hormonal and nutrient signals. Here I report that preferential expression of constitutively-active AMP-kinase (CA-AMPK) in the PVH blilaterally using lenti-virus vector (neuron specific-synapsin 1 promoter) increased food intake and body weight under lab chow diet. Furthermore, CA-AMPK mice increased the preference for high carbohydrate diet, by enhancing fatty acid oxidation in the PVH. In contrast, diet-induced as well as KKAy obese mice increased the preference for HFD, associated with paradoxically decreased AMPK activity and FAO in the PVH.
Thus, my results suggest that AMPK-FAO in the PVH regulates the preference for carbohydrate and fat diets. Furthermore, obese mice impair the AMPK-FAO system in the PVH and thereby increase HFD intake
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