The molecular mechanism and physiological significance of thyroid hormone non-genomic action

• Project/Area Number: 20790658
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Nagoya University
• Principal Investigator: SOU Kasumi Nagoya University, 環境医学研究所, 助教 (70432218)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 甲状腺ホルモン / ノンゲノミック作用PI3K→Akt / ノンゲノミック作用 / シグナル伝達 / 甲状腺ホルモン(T3) / nongenomic作用 / シグナリング伝達

Research Abstract

We have reported previously that thyroid hormone (T3) stimulates the PI3K/Akt pathway through thyroid hormone receptor (TR).It is a non-genomic action since the novel protein synthesis is not involved in. In the present study we demonstrated that T3 promotes the complex formation of TR/Src/p85, which induces the phosphorylation of Src and p85 sequentially and results in the activation of PI3K→Akt. This novel T3 action plays important role in neuronal cell survival.

Research Products

• [Journal Article] Thyroid-hormone-dependent activation of the phosphoinositide 3-kinase/Akt cascade requires Src and enhances neuronal survival. (2009)
  • Author(s): Cao X, Kambe F, Yamauchi M, Seo H.
  • Journal Title: Biochem J. 424(2) Pages: 201-209
• [Journal Article] Thyroid hormone non-genomically suppresses Src thereby stimulating osteocalcin expression in primary mouse calvarial osteoblasts. (2009)
  • Author(s): Asai S, Cao X, Yamauchi M, Funahashi K, Ishiguro N, Kambe F.
  • Journal Title: Biochem Biophys Res Commun. 387(1) Pages: 92-96
  • Peer Reviewed
• [Journal Article] Prostaglandin E2 negatively regulates AMP-activated protein kinase via protein kinase A signaling pathway. (2009)
  • Author(s): Funahashi K, Cao X, Yamauchi M, Kozaki Y, Ishiguro N, Kambe F.
  • Journal Title: Prostaglandins Other Lipid Mediat. 88(1-2) Pages: 31-35
• [Journal Article] Thyroid-hormone-dependent activation of the phosphoinositide 3-kinase/Akt cascade requires Src and enhances neuronal survival. (2009)
  • Author(s): Cao X, Kambe F, Yamauchi M, Seo H
  • Journal Title: Biochem J. 424(2) Pages: 201-209
• [Journal Article] Thyroid hormone non-genomically suppresses Src thereby stimulating osteocalcin expression in primary mouse calvarial osteoblasts. (2009)
  • Author(s): Asai S, Cao X, Yamauchi M, Funahashi K, Ishiguro N, Kambe F
  • Journal Title: Biochem Biophys Res Commun. 387(1) Pages: 92-96
  • Peer Reviewed
• [Journal Article] Prostaglandin E2 negatively regulates AMP-activated protein kinase via protein kinase A signaling pathway. (2009)
  • Author(s): Funahashi K, Cao X, Yamauchi M, Kozaki Y, Ishiguro N, Kambe F.
  • Journal Title: Prostaglandins Other Lipid Mediat. 88(1-2): Pages: 31-35
• [Journal Article] 3beta-Hydroxysteroid-delta24 redu-ctase is a hydrogen peroxide scavenger, protecting cells from oxidative stress-induced apoptosis. (2008)
  • Author(s): Lu X, Kambe F, Cao X, Kozaki Y, Kaji T, Ishii T, Seo H.
  • Journal Title: End-ocrinology. 149(7) Pages: 3267-73
  • Peer Reviewed
• [Journal Article] Thyroid hormone activates adenosine 5'-monophosphate-activated protein kina-se, via intracellular calcium mobilization and activ-ation of calcium/calmodulin-dependent protein kinase beta. (2008)
  • Author(s): Yamauchi M, Kambe F, Cao X, Lu X, Kozaki Y, Oiso Y, Seo H.
  • Journal Title: Mol Endocrinol. 22(4) Pages: 893-903
  • Peer Reviewed
• [Journal Article] Insulin-like grown factor-I activation of Akt survival cascade in neuronal cells requires the presence of its cognate receptor in caveolae. (2008)
  • Author(s): Lu X, Kambe F, Cao X, Yamauchi M, Seo H.
  • Journal Title: Exp Cell Res. 314(2) Pages: 342-51
• [Journal Article] 3 beta-Hydroxysteroid-delta 24 reductase is a hydrogen peroxide scavenger, protecting cells from oxidative stress-induced apoptosis (2008)
  • Author(s): Lu X, Kambe F, Cao X, Kozaki Y, Kaji T, Ishii T, Seo H.
  • Journal Title: Endocrinology 149(7) Pages: 3267-73
  • Peer Reviewed
• [Journal Article] Thyroid hormone activates adenosine 5'-monophosphate-activated protein kinase via intracellular calcium mobilization and activation of calcium/calmodulin-dependent protein kinase beta (2008)
  • Author(s): Yamauchi M, Kambe F, Cao X, Lu X, Kozaki Y, Oiso Y, Seo H.
  • Journal Title: Mol Endocrinol 22(4) Pages: 893-903
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor-I activation of Akt survival cascade in neuronal cells requires the presence of its cognate receptor in caveolae (2008)
  • Author(s): Lu X, Kambe F, Cao X, Ya mauchi M and Seo H.
  • Journal Title: Exp Cell Res. 314(2) Pages: 342-51