Gene expression analysis of bone marrow CD34 positive cells in patients with rheumatoid arthritis

• Project/Area Number: 20790695
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kitasato University
• Principal Investigator: HASHIMOTO Atsushi Kitasato University, 医学部, 助教 (50327376)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 関節リウマチ / 変形性関節症 / 骨髄 / FKBP5 / RAW264.7

Research Abstract

The expression of mRNA for FKBP5 was significantly higher in rheumatoid arthritis (RA) BM CD34+ cells than those in osteoarthritis (OA). FKBP5 stable transfectant of RAW 264.7 (Mouse leukemic monocyte macrophage cell line) was made and stimulated with TNF-α, resulted in up-regulation of NFkB and transformation to fibroblast-like cells. This is a possible pathway linking bone marrow and arthritis in RA patients. Identification of the gene associated with pathogenesis of RA such as FKBP5 can lead to new fundamental gene therapy of RA.

Research Products

• [Journal Article] Enhanced expression of mRNA for FK506-binding protein 5 in bone marrow CD34 positive cells in patients with rheumatoid arthritis. (2010)
  • Author(s): Matsushita R, Hashimoto A, Tomita T, Yoshitawa H, Tanaka S, Endo H, Hirohata S.
  • Journal Title: Clinical andExperimental Rheumatology Vol.28, No.1 Pages: 87-90
  • Peer Reviewed
• [Journal Article] Enhanced expression of mRNA for FK506-binding protein 5 in bone marrow CD34 positive cells in patients with rheumatoid arthritis. (2010)
  • Author(s): Matsushita R, Hashimoto A, et al.
  • Journal Title: Clinical and Experimental Rheumatology Volume: 28 Pages: 87-90
  • Peer Reviewed
• [Presentation] GENE EXPRESSION ANALYSIS OF BONE MARROW CD34 POSITIVE CELLS IN PATIENTS WITH RHEUMATOID ARTHRITIS. (2010)
  • Author(s): R.Matsushita, A.Hashimoto, S.Tanaka, H.Endo, S.Hirohata, T.Tomita, H.Yoshikawa.
  • Organizer: European League against Rheumatic Diseases2010
  • Place of Presentation: Rome (Abstract)
  • Year and Date: 2010-06-16
• [Presentation] 関節リウマチ骨髄におけるFKBP5のNFkB活性化への関与に関する解析 (2009)
  • Author(s): 松下礼子、橋本篤、飯塚進子、遠藤平仁、冨田哲也、吉川秀樹、廣畑俊成
  • Organizer: 第53回日本リウマチ学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-25
• [Presentation] 関節リウマチ骨髄における発現遺伝子の解析 (2008)
  • Author(s): 松下礼子、橋本篤、ほか
  • Organizer: アメリカリウマチ学会
  • Place of Presentation: アメリカ・サンフランシスコ
  • Year and Date: 2008-10-26
• [Presentation] 関節リウマチ骨髄における発現遺伝子の解析 (2008)
  • Author(s): 松下礼子、橋本篤、飯塚進子、木村美保、遠藤平仁、廣畑俊成、冨田哲也、吉川秀樹
  • Organizer: 第52回日本リウマチ学会総会・学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2008-04-21
• [Presentation] 関節リウマチ骨髄における発現遺伝子の解析 (2008)
  • Author(s): 松下礼子、橋本篤、ほか
  • Organizer: 日本リウマチ学会総会・学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2008-04-21