| Project/Area Number |
20790710
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Infectious disease medicine
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| Research Institution | Osaka University |
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Principal Investigator |
DAIDOJI Tomo Osaka University, 微生物病研究所, 特任研究員 (80432433)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
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| Keywords | 高病原性鳥インフルエンザ / アポトーシス / 高病原性トリインフルエンザ |
|---|
| Research Abstract |
To elucidate the mechanism of H5N1 pathogenesis, we prepared primary airway epithelial cells from alveolar tissues from 1-year-old pigs and generated recombinant H5N3 viruses possessing H5N1 hemagglutinin (HA) by using reverse genetics system. After infection with the recombinant H5N3 viruses, H5N1 HA protein played a critical role in inducing caspase-dependent apoptosis in infected porcine alveolar epithelial cells. In addition, H5N1 HA1 region (84-340 amino acids) was essential for induction of caspase-dependent apoptosis in infected cells.
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