| Project/Area Number |
20790731
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Hiroshima University |
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Principal Investigator |
OKADA Satoshi Hiroshima University, 病院, 病院助教 (80457241)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 免疫学 / 遺伝子 / 疾患モデルマウス / ELA2 / 重症先天性好中球減少症 |
|---|
| Research Abstract |
We tried to generate Severe Congenital Neutropenia (SCN) model mouse. We produced wild-type (WT), P110L, R101Q and C194X ELA2 by PCR-based mutagenesis and introduced them into retrovirus Tet-on gene expression vector. We performed gene expression experiment by using HEK293 cells. Although ELA2 expression was observed, we could not analyze them because the ELA2 introduced cells result in cell death. Now, we are trying to control ELA2 expression level by control the concentration of doxycycline. After that, we are planning generate SCN model mouse by BM reconstitution using CD34 positive cells from human cord blood.
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