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Defective erythropoiesis and ribosomal protein genes : Analysis of zebrafish model for Diamond-Blackfan anemia

Research Project

Project/Area Number 20790734
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionUniversity of Miyazaki

Principal Investigator

UECHI Tamayo  University of Miyazaki, フロンティア科学実験総合センター, 研究員 (10381104)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsリボソームタンパク質遺伝子 / 造血異常 / ゼブラフィッシュ / リボソームタンパク質 / ダイヤモンド・ブラックファン貧血 / モルフォリノアンチセンスオリゴ / 翻訳異常 / 赤芽球 / リボソーム病
Research Abstract

To investigate the pathogenic mechanism of Diamond-Blackfan anemia (DBA), I have developed a zebrafish DBA model by knocking down the ribosomal protein s19 gene using morpholino antisense oligo. Translational efficiency or production of total proteins in this model seemed almost same as control embryos. However, they showed the defective maturation of erythroid lineage by 2-3 days post fertilization. This suggests that there is an unknown mechanism that affect translation of specific factors, especially those involved in the erythroid pathway.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (15 results)

All 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (9 results)

  • [Journal Article] リボソーム病:翻訳装置のシステム障害2009

    • Author(s)
      上地珠代, 剣持直哉
    • Journal Title

      最新RNAと疾患研究(遺伝子医学MOOK15号)

      Pages: 79-84

    • Related Report
      2009 Final Research Report
  • [Journal Article] Loss of ribosomal protein L11 affects zebrafish embryonic development through a p53-dependent apoptotic response.2009

    • Author(s)
      Chakraborty A, Uechi T, Higa S, Torihara H, Kenmochi N.
    • Journal Title

      PLoS ONE. 4

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] レボソーム病:翻訳装置のシステム障害2009

    • Author(s)
      上地珠代
    • Journal Title

      遺伝子医学MOOk:最新RNAと疾患研究 18

      Pages: 79-84

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Loss of ribosomal protein L11 affects zebrafish embryonic development through a p53-dependent anoptotic resnonse2009

    • Author(s)
      Anirban Chakraborty
    • Journal Title

      PLoS ONE 4

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of ribosomal protein S19 during early embryogenesis leads to reduction of erythrocytes in a zebrafish model of Diamond-Blackfan anemia.2008

    • Author(s)
      Uechi T, Nakajima Y, Chakraborty A, Torihara H, Higa S, Kenmochi N.
    • Journal Title

      Hum Mol Genet. 17

      Pages: 3204-3211

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of ribosomal protein S19 during early embryogenesis leads to reduction of erythrocytes in a zebrafish model of Diamond-Blackfan anemia2008

    • Author(s)
      Tamayo Uechi
    • Journal Title

      Human Molecular Genetics 17

      Pages: 3204-3211

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] リボソームの翻訳異常とダイヤモンド・ブラックファン貧血:ゼブラフィッシュを用いた解析2009

    • Author(s)
      上地珠代
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Loss of ribosomal protein s19 leads to defective erythropoiesis in a zebrafish model of Diamond-Blackfan anemia2009

    • Author(s)
      上地珠代
    • Organizer
      Ribosomal Synthesis
    • Place of Presentation
      レーゲンスブルグ,ドイツ
    • Year and Date
      2009-08-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] リボソームの翻訳異常とダイヤモンド・ブラックファン貧血2009

    • Author(s)
      上地珠代, 中島由香里, 鳥原英嗣, 剣持直哉.
    • Organizer
      ゼブラフィッシュを用いた解析日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] Loss of ribosomal protein s19 leads to defective erythropoiesis in a zebrafish model of Diamond-Blackfan anemia Ribosome Synthesis2009

    • Author(s)
      Uechi T, Nakajima Y, Torihara H, Chakraborty A, Kenmochi N.
    • Organizer
      Regensburg
    • Place of Presentation
      ドイツ
    • Related Report
      2009 Final Research Report
  • [Presentation] 造血異常とリボソームタンパク質遺伝子の変異:ゼブラフィッシュを用いた解析2009

    • Author(s)
      上地珠代, 中島由香里, 鳥原英嗣, 剣持直哉
    • Organizer
      日本RNA学会
    • Place of Presentation
      新潟
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 翻訳異常とダイヤモンド・ブラックファン貧血 : ゼブラフィッシュを用いた解析2008

    • Author(s)
      上地珠代
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] リボソームタンパク質の変異と疾患〜ダイヤモンド・ブラックファン貧血モデルの解析〜2008

    • Author(s)
      上地珠代
    • Organizer
      日本RNA学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-07-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] アニルバンチャクラボルティ, 比嘉三代美, 剣持直哉. 翻訳異常とダイヤモンド・ブラックファン貧血:ゼブラフィッシュモデルを用いた解析2008

    • Author(s)
      上地珠代, 中島由香里, 鳥原英嗣
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Presentation] アニルバンチャクラボルティ, 比嘉三代美, 剣持直哉. リボソームタンパク質の変異と疾患:ダイヤモンド・ブラックファン貧血モデルの解析2008

    • Author(s)
      上地珠代, 中島由香里, 鳥原英嗣
    • Organizer
      日本RNA学会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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