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Detection of imprinting abnormalities in rhabdomyosarcoma patients using tumor and serum DNA.

Research Project

Project/Area Number 20790738
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

KIKUCHI Ken  Kyoto Prefectural University of Medicine, 医学研究科, 助教 (40453104)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords横紋筋肉腫 / インプリンティング / IGF2 / 血清遊離DNA / H19 / 血清DNA
Research Abstract

In RMS cell lines and RMS tumor samples, we were able to detect a methylation abnormality in the imprinting center, in other words, imprinting abnormalities in IGF2 and H19 by Melting Curve Analysis. In RMS tumor samples, it was suggested that imprinting abnormalities correlate with the expression of IGF2 mRNA. Furthermore, we established a method to detect imprinting abnormalities in IGF2 and H19 in serum DNA.

Report

(3 results)
  • 2010 Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (5 results)

All 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Restoration of p53 pathway by nutlin-3 induces cell cycle arrest and apoptosis in human rhabdomyosarcoma cells.2009

    • Author(s)
      Miyachi M, Kakazu N, Yagyu S, Katsumi Y, Tsubai-Shimizu S, Kikuchi K, Tsuchiya K, Iehara T, Hosoi H.
    • Journal Title

      Clinical cancer research. 15

      Pages: 4077-4084

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Restoration of p53 pathway by nutlin-3 induces cell cycle arrest and apoptosis in human rhabdomyosarcoma cells.2009

    • Author(s)
      Miyachi M, Kakazu N, Yagyu S, Katsumi Y, Tsubai-Shimizu S, Kikuchi K, Tsuchiya K, Iehara T, Hosoi H.
    • Journal Title

      Clinical cancer research 15

      Pages: 4077-4084

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 横紋筋肉腫患者血清遊離DNAを用いたIGF2/H19 imprinting異常の検出2008

    • Author(s)
      菊地顕
    • Organizer
      第111回日本小児科学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-04-26
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Detection of IGF2 and H19 imprinting abnormalities in rhabdomyosarcoma patients using tumor and serum DNA.2008

    • Author(s)
      Kikuchi K.
    • Organizer
      40th International Society of Pediatric Oncology.
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2010 Final Research Report
  • [Presentation] Detection of IGF2 and H19 imprinting abnormalities in rhabdomyosarcoma patients using tumor and serum DNA.2008

    • Author(s)
      Ken Kikuchi
    • Organizer
      40th International Society of Pediatric Oncology
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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