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Abnormalities in cell polarity in polycystic kidney disease

Research Project

Project/Area Number 20790739
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionWakayama Medical University

Principal Investigator

TOGAWA Hiroko  Wakayama Medical University, 医学部, 助教 (30445093)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords多発性嚢胞腎 / 遺伝性腎疾患 / 上皮間葉移行 / PCKラット / 間葉系マーカー / E-カドヘリン / βカテニン / エンドサイトーシス / 細胞極性 / Na^+-K^+-ATPase / Na^<+->K^<+->ATPase
Research Abstract

We examined the expression of cell adhesion molecules and mesenchymal markers to clarify a loss of cell polarity in PCK rat, an ARPKD model with a mutation in the orthologue of human PKHDJ. E-cadherin and β-catenin expression levels in cystic tubule was attenuated and localized to lateral cell-cell contacts according to cyst enlargement in PCK rats. Some epithelial cells in large cysts derived from these segments of nephron, especially in adjacent fibrotic area, showed positive immunoreactivity of vimentin and fibronectin. Vimentin and fibronectin positive cells are increased in fibrotic area around cyst. In conclusions, these findings suggested that EMT is involved in pathophysiology of PKD.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (18 results)

All 2011 2010 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (12 results)

  • [Journal Article] Epithelial-to-mesenchymal transition in cyst-lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease.2011

    • Author(s)
      Togawa H, Nakanishi K, Mukaiyama H, Hama T, Shima Y, Sako M, Miyajima M, Nozu K, Nishii K, Nagao S, Takahashi H, Iijima K, Yoshikawa N.
    • Journal Title

      Am J Physiol Renal Physiol. 300(2)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epithelial-to-mesenchymal transition in cyst-lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease.2011

    • Author(s)
      Togawa H
    • Journal Title

      Am J Physiol Renal Physiol

      Volume: 300

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased chymase-positive mast cells in children with crescentic glomerulonephritis.2009

    • Author(s)
      Togawa H
    • Journal Title

      Pediatric Nephrology 24

      Pages: 1071-1075

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased chymase-positive mast cells in children with crescentic glomerulonephritis2009

    • Author(s)
      Togawa H
    • Journal Title

      Pediatr Nephrol 24

      Pages: 1071-1075

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Membranous nephropathy associated with thyroid-peroxidase antigen2009

    • Author(s)
      Shima Y
    • Journal Title

      Pediatr Nephrol 24

      Pages: 605-608

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved renal survival in Japanese children with IgA nephropathy2008

    • Author(s)
      Yata N
    • Journal Title

      Pediatr Nephrol 23

      Pages: 905-912

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Epithelial-to-Mesenchymal Transition (EMT) in cpk Mouse, a Model of ARPKD.2010

    • Author(s)
      Togawa H, Nakanishi K, Mukaiyama H, Hama T, Shima Y, Miyajima M, Nozu K, Takahashi H, Nagao S, Iijima K, Yoshikawa N.
    • Organizer
      43^<th> Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Denver, USA
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 常染色体劣性多発性嚢胞腎(ARPKD)モデルcpkマウスの尿細管上皮細胞における上皮間葉移行(EMT)2010

    • Author(s)
      戸川寛子, 中西浩一, 島友子, 佐古まゆみ, 向山弘展, 宮嶋正康, 野津寛大, 吉原大輔, 長尾枝澄香, 飯島一誠, 吉川徳茂
    • Organizer
      第44回日本小児腎臓病学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] ヒトARPKDと相同遺伝子変異を有するPCK ratにおける上皮間葉移行2010

    • Author(s)
      戸川寛子, 中西浩一, 島友子, 佐古まゆみ, 向山弘展, 宮嶋正康, 野津寛大, 吉原大輔, 長尾枝澄香, 飯島一誠, 吉川徳茂
    • Organizer
      第53回日本腎臓学会学術総会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] ARPKD相同遺伝子ラットモデルの尿細管上皮細胞における上皮間葉移行(EMT).2009

    • Author(s)
      戸川寛子
    • Organizer
      第44回日本小児腎臓病学会学術集会
    • Place of Presentation
      一橋記念講堂・学術センター(東京都)
    • Year and Date
      2009-06-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒトARPKD相同遺伝子ラットモデルにおける尿細管部位特異的嚢胞形成.2009

    • Author(s)
      戸川寛子
    • Organizer
      第52回日本腎臓学会学術総会
    • Place of Presentation
      パシイフイコ横浜(神奈川県)
    • Year and Date
      2009-06-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] Snaill Is Involved in Epithelial-to-Mesenchymal Transition (EMT) in Cyst-Lining Epithelial.2009

    • Author(s)
      Togawa H, Nakanishi K, Shima Y, Sako M, Miyajima M, Nozu K, Iijima K, Yoshihara D, Nagao S, Yoshikawa N
    • Organizer
      42^<th> Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      San Diego, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] ARPKD相同遺伝子ラットモデルの尿細管上皮細胞における上皮間葉移行(EMT)2009

    • Author(s)
      戸川寛子, 中西浩一, 島友子, 佐古まゆみ. 宮嶋正康, 野津寛大, 飯島一誠, 吉原大輔, 長尾枝澄香, 吉川徳茂
    • Organizer
      第44回日本小児腎臓病学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒトARPKD相同遺伝子ラットモデルにおける尿細管部位特異的嚢胞形成2009

    • Author(s)
      戸川寛子, 中西浩一, 島友子, 佐古まゆみ, 宮嶋正康, 西井一宏, 長尾枝澄香, 高橋久英, 吉川徳茂
    • Organizer
      第52回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Snail1 Is Involved in Epithelial-to-Mesenchymal Transition (EMT) in Cyst-Lining Epithelial Cells in PCK Rat.2009

    • Author(s)
      Togawa H
    • Organizer
      42^<th> Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      San Diego, USA
    • Related Report
      2009 Annual Research Report
  • [Presentation] Epithelial-to-mesenchymal transition (EMT) in cyst-lining epithelial cells in PCK rat2008

    • Author(s)
      Hiroko Togawa
    • Organizer
      The 41th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2008-11-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] Epithelial-to-Mesenchymal Transition in Cyst-Lining Epithelial Cells in PCK Rat. J Am Soc Nephrol 19 : 362A2008

    • Author(s)
      Togawa H, Nakanishi K, Obana M, Shima Y, Miyajima M, Nozu K, Iijima K, Nishi K, Nagao S, Takahashi H, Yoshikawa N
    • Organizer
      2008 41^<th> Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] 多発性嚢胞腎(PKD)の嚢胞形成にはE-cadherin/β-cateninの破綻が関与する2008

    • Author(s)
      戸川寛子, 中西浩一, 佐古まゆみ, 尾鼻美奈, 島友子, 宮嶋正康, 西井一宏, 長尾枝澄香, 高橋久英, 吉川徳茂
    • Organizer
      第43回日本小児腎臓病学会学術集会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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