Abnormalities in cell polarity in polycystic kidney disease
| Project/Area Number |
20790739
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
TOGAWA Hiroko Wakayama Medical University, 医学部, 助教 (30445093)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 多発性嚢胞腎 / 遺伝性腎疾患 / 上皮間葉移行 / PCKラット / 間葉系マーカー / E-カドヘリン / βカテニン / エンドサイトーシス / 細胞極性 / Na^+-K^+-ATPase / Na^<+->K^<+->ATPase |
|---|
| Research Abstract |
We examined the expression of cell adhesion molecules and mesenchymal markers to clarify a loss of cell polarity in PCK rat, an ARPKD model with a mutation in the orthologue of human PKHDJ. E-cadherin and β-catenin expression levels in cystic tubule was attenuated and localized to lateral cell-cell contacts according to cyst enlargement in PCK rats. Some epithelial cells in large cysts derived from these segments of nephron, especially in adjacent fibrotic area, showed positive immunoreactivity of vimentin and fibronectin. Vimentin and fibronectin positive cells are increased in fibrotic area around cyst. In conclusions, these findings suggested that EMT is involved in pathophysiology of PKD.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Epithelial-to-mesenchymal transition in cyst-lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease.2011
Author(s)
Togawa H, Nakanishi K, Mukaiyama H, Hama T, Shima Y, Sako M, Miyajima M, Nozu K, Nishii K, Nagao S, Takahashi H, Iijima K, Yoshikawa N.
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Journal Title
Am J Physiol Renal Physiol. 300(2)
Related Report
Peer Reviewed
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[Presentation] Epithelial-to-Mesenchymal Transition (EMT) in cpk Mouse, a Model of ARPKD.2010
Author(s)
Togawa H, Nakanishi K, Mukaiyama H, Hama T, Shima Y, Miyajima M, Nozu K, Takahashi H, Nagao S, Iijima K, Yoshikawa N.
Organizer
43^<th> Annual Meeting of the American Society of Nephrology
Place of Presentation
Denver, USA
Related Report
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[Presentation] 常染色体劣性多発性嚢胞腎(ARPKD)モデルcpkマウスの尿細管上皮細胞における上皮間葉移行(EMT)2010
Author(s)
戸川寛子, 中西浩一, 島友子, 佐古まゆみ, 向山弘展, 宮嶋正康, 野津寛大, 吉原大輔, 長尾枝澄香, 飯島一誠, 吉川徳茂
Organizer
第44回日本小児腎臓病学会学術集会
Place of Presentation
大阪
Related Report
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[Presentation] ヒトARPKDと相同遺伝子変異を有するPCK ratにおける上皮間葉移行2010
Author(s)
戸川寛子, 中西浩一, 島友子, 佐古まゆみ, 向山弘展, 宮嶋正康, 野津寛大, 吉原大輔, 長尾枝澄香, 飯島一誠, 吉川徳茂
Organizer
第53回日本腎臓学会学術総会
Place of Presentation
神戸
Related Report
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[Presentation] Snaill Is Involved in Epithelial-to-Mesenchymal Transition (EMT) in Cyst-Lining Epithelial.2009
Author(s)
Togawa H, Nakanishi K, Shima Y, Sako M, Miyajima M, Nozu K, Iijima K, Yoshihara D, Nagao S, Yoshikawa N
Organizer
42^<th> Annual Meeting of the American Society of Nephrology
Place of Presentation
San Diego, USA
Related Report
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[Presentation] Epithelial-to-Mesenchymal Transition in Cyst-Lining Epithelial Cells in PCK Rat. J Am Soc Nephrol 19 : 362A2008
Author(s)
Togawa H, Nakanishi K, Obana M, Shima Y, Miyajima M, Nozu K, Iijima K, Nishi K, Nagao S, Takahashi H, Yoshikawa N
Organizer
2008 41^<th> Annual Meeting of the American Society of Nephrology
Place of Presentation
Philadelphia, USA
Related Report
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