| Project/Area Number |
20790740
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
SHIMA Yuko Wakayama Medical University, 医学部, 助教 (60433364)
|
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
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| Keywords | ネフローゼ症候群 / 小胞体ストレス / シクロスポリン / ネフリン / 小胞体関連分解 / アポトーシス |
|---|
| Research Abstract |
To clarify the presence of ER stress and a role of it in children with nephrotic syndrome, we obtained renal biopsy specimens in children with nephrotic syndrome. We performed real-time PCR to investigate the presence of ER stress and apoptosis. We analyzed EIF2α, GRP78, GRP94, CHOP and BAD. The amount of each gene amplification was examined before and after the administration of cyclosporine. The patients with cyclosporine showed significant increase of amplification of GRP78, GRP94, BAD, and EIF2α, suggesting that the use of cyclosporine is a factor provoking ER stress. The amplification of BAD increased significantly after the administration of cyclosporine, suggesting that the use of cyclosporine may induce apoptosis through ER stress.
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