Analysis of rotavirus antigenemia in hepatopoietic stem cell transplanted children

• Project/Area Number: 20790752
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Fujita Health University
• Principal Investigator: SUGATA Ken Fujita Health University, 医学部, 助教 (60454401)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: ロタウイルス / 抗原血症 / 免疫抑制状態 / 造血幹細胞移植 / 抗RV IgG抗体 / HSCT

Research Abstract

We've already reported that rotavirus antigenemia has been found in immunocompetent rotavirus gastroenteritis patients. However, the pathogenesis of rotavirus infection in immunocompromised transplant recipients remains unclear. Serially collected serum samples obtained from the patients receiving allogeneic hematopoietic stem cell transplants were measured rotavirus antigen levels using a previously established enzyme-linked immunosorbent assay.
Rotavirus antigenemia occurred in hematopoietic stem cell transplant (HSCT) recipients. Although the duration of antigenemia was clearly longer than in immunocompetent rotavirus gastroenteritis patients, the levels of antigen were not as high.

Research Products

• [Journal Article] Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients. (2010)
  • Author(s): Ihira M, Sugiyama H, Enomoto Y, Higashimoto Y, Sugata K, Asano Y, Yoshikawa T.
  • Journal Title: J Virol Methods. 167(1) Pages: 103-106
  • Peer Reviewed
• [Journal Article] Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients. (2010)
  • Author(s): Ihira M, et al.
  • Journal Title: J Virol Methods Volume: 167 Pages: 103-106
  • Peer Reviewed
• [Journal Article] Evaluation of reverse transcription loop-mediated isothermal amplification assays for rapid diagnosis of pandemic influenza A/H1N1 2009 virus. (2010)
  • Author(s): Nakauchi M, et al.
  • Journal Title: J Med Virol Volume: 83 Pages: 10-15
  • Peer Reviewed
• [Journal Article] 単純ヘルペス感染症 (2010)
  • Author(s): 菅田健
  • Journal Title: 小児内科 Volume: 42 Pages: 171-175
• [Journal Article] Single episode of Behcet's disease-like symptoms caused by herpes simplex virus reactivation. (2009)
  • Author(s): Sugata K, Enomoto Y, Sugiyama H, Fujita A, Miyake F, Asano Y, Yoshikawa.
  • Journal Title: Peditr Int. 51(4) Pages: 577-578
  • NAID: 10025347817
  • Peer Reviewed
• [Journal Article] Exanthem subitum-associated encephalitis : nationwide survey in Japan. (2009)
  • Author(s): Yoshikawa T, Ohashi M, Miyake F, Fujita A, Usui C, Sugata K, Suga S, Hashimoto S, Asano Y.
  • Journal Title: Pediatr Neurol. 41(5) Pages: 353-358
  • Peer Reviewed
• [Journal Article] Single episode of Behcet's disease-like symptoms caused by herpes simplex virus reactivation (2009)
  • Author(s): Sugata K et al.
  • Journal Title: Pediatr Int 51 Pages: 577-578
  • NAID: 10025347817
  • Peer Reviewed
• [Journal Article] Exanthem subitum-associated encephalitis : nationwide survey in Japan (2009)
  • Author(s): Yoshikawa T et al.
  • Journal Title: Pediatr Neurol 41 Pages: 353-358
  • Peer Reviewed
• [Journal Article] Analysis of rotavirus antigenemia and extraintestinal manifestations in children with rotavirus gastroenteritis. (2008)
  • Author(s): Sugata K, Taniguchi K, Yui A, Miyake F, Suga S, Asano Y, Ohashi M, Suzuki K, Nishimura N, Ozaki T, Yoshikawa T.
  • Journal Title: Pediatr. 22(2) Pages: 392-397
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification for discriminating between human herpesvirus 6A and B. (2008)
  • Author(s): Ihira M, Ohta A, Sugata K, Suga S, Asano Y, Yoshikawa T.
  • Journal Title: J Virol Methods. 154(1-2) Pages: 223-225
  • Peer Reviewed
• [Journal Article] Elevated serum cytokine levels are associated with human herpesvirus 6 reactivation in hematopoietic stem cell transplantation recipients. (2008)
  • Author(s): Fujita A, Ihira M, Suzuki R, Enomoto Y, Sugiyama H, Sugata K, Suga S, Asano Y, Yagasaki H, Kojima S, Matsumoto K, Kato K, Yoshikawa T.
  • Journal Title: J.Infect. 57(3) Pages: 241-248
  • Peer Reviewed
• [Journal Article] Human herpesvirus 6 infection in adult living related liver transplant recipients. (2008)
  • Author(s): Ohashi M, Sugata K, Ihira M, Asano Y, Egawa H, Takada Y, Uemoto S, Yoshikawa T.
  • Journal Title: Liver Transpl. 14(1) Pages: 100-109
  • Peer Reviewed
• [Journal Article] Analysis of rotavirus antigenemia and extraintestinal manifestations in children with rotavirus gastroenteritis. (2008)
  • Author(s): Sugata K, et.al.
  • Journal Title: Pediatr 122 Pages: 392-7
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B. (2008)
  • Author(s): Ihira M, et.al.
  • Journal Title: J Virol Methods 154 Pages: 223-5
  • Peer Reviewed
• [Journal Article] Elevated serum cytokine levels are associated with human herpesvirus 6 reactivation in hematopoietic stem cell transplantation recipients (2008)
  • Author(s): Fujita A, et.al.
  • Journal Title: J Infect 57 Pages: 241-8
  • Peer Reviewed
• [Journal Article] Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients
  • Author(s): Ihira M et al
  • Journal Title: J Virol Methods (In press)
  • Peer Reviewed
• [Presentation] 造血幹細胞移植患児で観察された長期間、低レベルロタウイルス抗原血症の病態解明 (2010)
  • Author(s): 菅田健
  • Organizer: 第58回日本ウイルス学会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] 造血幹細胞移植患児で観察された長期間、低レベルロタウイルス抗原血症の病態解明 (2010)
  • Author(s): 菅田健
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-07
• [Presentation] 膠原病患者における帯状疱疹発症リスクの解析:水痘帯状疱疹ウイルスELISPOTアッセイによる特異的細胞性免疫能の評価 (2010)
  • Author(s): 菅田健
  • Organizer: 第25回ヘルペスウイルス研究会
  • Place of Presentation: 浜松
  • Year and Date: 2010-05-27
• [Presentation] 造血器腫瘍の化学療法中にBKV感染を認めた2小児例 (2009)
  • Author(s): 菅田健
  • Organizer: 第41回日本小児感染症学会
  • Place of Presentation: 福井
  • Year and Date: 2009-11-15
• [Presentation] 免疫不全患児におけるロタウイルス感染症の病態解明:院内感染リスクの高い小児病棟内での状況把握 (2009)
  • Author(s): 菅田健
  • Organizer: 第57回日本ウイルス学会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-26
• [Presentation] Factors in association with three important herpesviruses (CMV, HHV-6, and EBV) reactivation after hematopoietic stem cell transplant. (2009)
  • Author(s): Ken Sugata
  • Organizer: 34th Annual International Herpesvirus Workshop
  • Place of Presentation: USA Ithaca
  • Year and Date: 2009-07-29
• [Presentation] Factors in association with three important herpesviruses(CMV, HHV-6, and EBV)reactivation after hematopoietic stem cell transplant (2009)
  • Author(s): Sugata K
  • Organizer: IDSA(Infectious Diseases Society of America)
  • Place of Presentation: Ithaca, NY
• [Presentation] Analysis of rotavirus antigenemia in hematopoietic stem cell transplant recipients. (2008)
  • Author(s): Ken Sugata
  • Organizer: IDSA
  • Place of Presentation: USA Washington DC
  • Year and Date: 2008-10-28
• [Presentation] Norovirus胃腸炎による高度脱水で死亡した1小児例 (2008)
  • Author(s): 菅田健
  • Organizer: 第44回中部日本小児科学会
  • Place of Presentation: 富山
  • Year and Date: 2008-08-24
• [Presentation] Analysis of rotavirus antigenemia in hematopoietic stem cell transplant recipients. (2008)
  • Author(s): Ken Sugata
  • Organizer: 4^<th> ACPID
  • Place of Presentation: Indonesia Surabaya
  • Year and Date: 2008-07-04
• [Presentation] 造血幹細胞移植患児におけるロタウイルス抗原血症の解析 (2008)
  • Author(s): 菅田健
  • Organizer: 第49回日本臨床ウイルス学会
  • Place of Presentation: 犬山
  • Year and Date: 2008-06-15
• [Presentation] Analysis of rotavirus antigenemia in hematopoietic stem cell transplant recipients (2008)
  • Author(s): Sugata K
  • Organizer: 46<th>^ Infectious Diseases Society of America
  • Place of Presentation: Washington, Washington DC