analysis of function about keratinocyte differentiation of p51/p63, homologue of tumor suppressor gene p53

• Project/Area Number: 20790786
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Tohoku University
• Principal Investigator: OKAWA Eisaku Tohoku University, 大学院・医学系研究科, 助教 (20451586)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 発生・分化 / 表皮細胞分化 / p51 / p63 / p53

Research Abstract

I found that p63 was related to the mechanism of keratin 1 expression in keratinocyte differentiation. p63 induces keratin 1 via JNK/AP-1 activation. I explained keratinocyte growth factor, from dermal fibroblast, suppressed keratin 1 expression via ERK signaling.I examined the relationship between p63 and sonic hedgehog. I overexpressed p63 in keratinocyte and showed the promoter activity of sonic hedgehog increased. In vivo, I showed that p63 expressed strongly in basal cell carcinoma by immunostaining method.

Research Products

• [Journal Article] Ectopic expression of the p53 homologue p63 is linked to squamous metaplasia in extramammary Paget's disease with invasive adenocarcinoma. (2009)
  • Author(s): E Ogawa, R Okuyama, T Egawa, H Nagoshi, H Tagami, S Ikawa, S Aiba
  • Journal Title: Histopathology 54 Pages: 374-393
  • Peer Reviewed
• [Journal Article] Cutaneous accumulation of abnormal polyglutamine proteins of patients with dentatorubral-pallidoluysian atrophy (2009)
  • Author(s): Ohta M, Okuyama R, Ogawa E, Kisu K, Sato H, Aoki M, Aiba S.
  • Journal Title: European Journal of Neurology Vol.16 Pages: 1246-1249
  • Peer Reviewed
• [Journal Article] p63/p51-induced Onset of Keratinocyte Differentiation via the c-Jun N-terminal Kinase Pathway Is Counteracted by Keratinocyte Growth Factor. (2008)
  • Author(s): E Ogawa, R Okuyama, T Egawa, H Nagoshi, M Obinata, H Tagami, S Ikawa, S Aiba
  • Journal Title: THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 283,NO.49 Pages: 34241-34249
  • Peer Reviewed
• [Journal Article] p63/p51induced Onset of Keratinocyte Differentiation via the c-Jun N-terminal Kinase Pathway Is Counteracted by Keratinocyte Growth Factor (2008)
  • Author(s): Eisaku Ogawa
  • Journal Title: The Journal of Biological Chemistry 283 Pages: 34241-34249
  • Peer Reviewed
• [Presentation] p63 prevents growth arrest via Sonic hedgehog induction (2009)
  • Author(s): 小川英作、奥山隆平、岡田修子、井川俊太郎、相場節也
  • Organizer: 日本研究皮膚科学会第34回年次学術大会・総会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-04
• [Presentation] Notch2 down-regulation in wound healing is contributed to acceleration of cell motility (2009)
  • Author(s): 奥山隆平、斉藤るみ子、小川英作、井川俊太郎、相場節也
  • Organizer: 日本研究皮膚科学会第34回年次学術大会・総会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-04