A role of nuclear import activity on the pathomechanism of cutaneous diseases

• Project/Area Number: 20790800
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: UMEGAKI Noriko Osaka University, 医学系研究科, 助教 (80397629)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 核移行シグナル / karyopherin α2 / TAP methods / mass spectrometry / karyopherin α / HGF / 増殖 / PCNA / LC / MS / プロモーター解析 / 皮膚炎症・再生学 / 核移次行シグナル

Research Abstract

Detection and analysis of target proteins imported by karyopherin alpha 2 (KPNA2) in HaCaT cells using tandem affinity purification method and mass spectrometry to investigate the roles of KPNA2 in the cell growth. Immunohistochemical staining using anti-KPNA2 antibody was performed in the skin diseases including psoriasis and squamous cell carcinomas to demonstrate the relation ship between KPNA2 and keratinocyte proliferation.

Research Products

• [Journal Article] Good's syndrome (hypogammaglobulinemia with thymoma) presenting intractable opportunistic infections and hyperkeratotic lichen planus (2010)
  • Author(s): Hanafusa T, Umegaki N, Ymaguchi Y, Katayama I
  • Journal Title: Journal of Dermatology 37 Pages: 171-174
  • NAID: 10027065837
  • Peer Reviewed
• [Journal Article] Good's syndrome (hypogammaglobulinemia with thymoma) presenting intractable onnortunistic infections and hyperkeratotic lichen planus. (2010)
  • Author(s): Hanafusa T, Umegaki N, Ymaguchi Y, Katayama I
  • Journal Title: Journal of Dermatology 37 Pages: 171-174
  • Peer Reviewed
• [Journal Article] Novel and recurrent nonsense mutation of the SLC39A4 gene in Japanese patients with acrodermatitis enteropathica (2009)
  • Author(s): Nakano H, Nakamura Y, Kawamura T, Shibagaki N, Matsue H, Aizu T, Rokunohe D, Akasaka E, Kimura K, Nishizawa A, Umegaki N, Mitsuhashi Y, Shimada S, Sawamura D.
  • Journal Title: Br J Dermatol 161(1) Pages: 184-6
  • Peer Reviewed
• [Journal Article] 強皮症様症状を呈し、突然死した全身性アミロイドーシスの1 (2009)
  • Author(s): 梅垣知子、長澤智彦、大畑千佳、中村敏明、片山一朗、西本憲弘、光定伸浩
  • Journal Title: 臨床皮膚科 63(12) Pages: 940-4
• [Journal Article] かゆみを伴う皮膚疾患患者での労働生産性の評価とヒスタミンH1拮抗薬による改善効果の検討 (2009)
  • Author(s): 室田浩之、北場俊、谷守金田眞理梅垣知子、片山一朗
  • Journal Title: Progress in Medicine 29 Pages: 1842-1848
  • Peer Reviewed
• [Journal Article] Novel and recurrent nonsense mutation of the SLC39A4 gene in Japanese patients with acrodermatitis enteropathica. (2009)
  • Author(s): Nakano H, Nakamura Y, Kawamura T, Shibagaki N, Matsue H, Aizu T, Rokunohe D, Akasaka E, Kimura K, Nishizawa A, Umegaki N, Mitsuhashi Y, Shimada S, Sawamura D.
  • Journal Title: Br J Dermatol. 161(1) Pages: 184-186
  • Peer Reviewed
• [Journal Article] かゆみを伴う皮膚疾患患者での労働生産性の評価とヒスタミンH1拮抗薬による改善効果の検討 (2009)
  • Author(s): 室田浩之、北場俊、谷守金田眞理, 梅垣知子、片山一朗
  • Journal Title: Progress in Medicine 29 Pages: 1842-1848
• [Journal Article] 強皮症様症状を呈し、突然死した全身性アミロイドーシスの1例 (2009)
  • Author(s): 梅垣知子、長澤智彦、大畑千佳、中村敏明、片山一朗、西本憲弘、光定伸浩
  • Journal Title: 臨床皮膚科 63 Pages: 940-944
  • Peer Reviewed
• [Journal Article] 遠心分離法による白血球除去療法が有効であった難治性壊疽性膿皮症の1例 (2008)
  • Author(s): 花房崇明、梅垣知子、山口裕史、片山一朗
  • Journal Title: 皮膚科の臨床 50 Pages: 871-874
  • Peer Reviewed
• [Journal Article] A novel homozygous missense mutation in the fatty aldehyde dehydrogenase gene causes Sjogren-Larsson syndrome (2008)
  • Author(s): Nakano H, Akasaka E, Rokunohe D, Yokoyama S, Toyomaki Y, Umegaki N. Mitsuhashi Y, Sawamura D.
  • Journal Title: J Dermatol Sci 52(2) Pages: 136-8
  • NAID: 10024457607
  • Peer Reviewed
• [Journal Article] New aspect of anti-inflammatory action of lipo-prostaglandinEl in the management of collagen diseases-related skin ulcer (2008)
  • Author(s): Murota H, Kotobuki Y, Umegaki N, Tani M, Katayama I.
  • Journal Title: Rheumatol Int 28(11) Pages: 1127-35
  • Peer Reviewed
• [Presentation] ヒト化抗IL-6受容体モノクローナル抗体(アクテムラ)療法を試みた関節症性乾癬の1例 (2009)
  • Author(s): 梅垣知子、吉良正浩、片山一朗、緒方篤
  • Organizer: 24回乾癬学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-04
• [Presentation] A case of recessive dystrophic epidermolysis bullosa complicated with dilated cardiomyopathy and fast-growing squamous cell carcinoma producing PTH-rP and G-CSF (2008)
  • Author(s): Noriko UMEGAKI^1, Katsuto TAMAI^2, Sayaka NASUNO^3, Shigetoyo KOGAKI^3, Keiichi OZONO^3, Ichiro KATAVAMA^1
  • Organizer: 第10回日本中国合同皮膚科学会
  • Place of Presentation: 杭州
  • Year and Date: 2008-11-01