Molecular analysis of mechanisms regulating drug sensitivity of lymphoid neoplasms to HDAC inhibitor

• Project/Area Number: 20790802
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Okayama University
• Principal Investigator: FUJII Kazuyasu Okayama University, 岡山大学病院, 助教 (70452571)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: HDAC阻害剤 / 薬剤感受性 / ヒストン脱アセチル化酵素阻害剤 / プロテオーム / 2D-DIGE / HDAC阻害薬 / バルプロ酸 / NK細胞

Research Abstract

To elucidate the molecular mechanisms and the predictive markers of sensitivity to histone deacetylase inhibitor (HDACi), we compared with IC50 values for valproic acid, one of the HDACi, in 37 lymphoid cell lines and their 2D-DIGE expression profiles. We newly identified 19 protein spots associated with HDACi sensitivity.

Research Products

• [Journal Article] Erythema multiforme-like contact reaction due to liquid-formulated 2, 2-dibromo-3-nitrilopropionamide: involvement of cytotoxic T-lymphocyte reaction. (2009)
  • Author(s): Nakanishi G, Fujii K, Asagoe K, Tanaka T, Iwatsuki K.
  • Journal Title: Clin Exp Dermatol. vol.39 Pages: 732-736
  • Peer Reviewed
• [Journal Article] 【T細胞腫瘍】皮膚T細胞リンパ腫菌状息肉症/Sezary症候群と原発性皮膚CD30陽性リンパ増殖症. (2009)
  • Author(s): 藤井一恭、岩月啓氏
  • Journal Title: 血液・腫瘍科 58巻 Pages: 686-693
• [Journal Article] Erythema multiforme-like contact reaction due to liquid-formulated 2,2-dibromo-3-nitrilopropionamide : involvement of cytotoxic T-lymphocyte reaction. (2009)
  • Author(s): Sennh A
  • Journal Title: Clin Exp Dermatol. 34 Pages: 732736-732736
  • Peer Reviewed
• [Journal Article] Human papillomavirus genome integration in multifocal vulvar Bowen"s disease and squamous cell carcinoma. (2009)
  • Author(s): Nakanishi G
  • Journal Title: Clin Exp Dermatol. 34 Pages: 965967-965967
  • Peer Reviewed
• [Journal Article] 【T細胞腫瘍】皮膚T細胞リンパ腫菌状息肉症/Sezary症候群と原発性皮膚CD30陽性リンパ増殖症 (2009)
  • Author(s): 藤井一恭
  • Journal Title: 血液・腫瘍科 58 Pages: 686693-686693
• [Presentation] 心筋浸潤を合併した重症型種痘様水疱症:副腎皮質ステロイドとVP16間歇療法による治療例. (2010)
  • Author(s): 平井陽至
  • Organizer: 第20回EBウイルス研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-06
• [Presentation] 心筋浸潤を合併した重症型種痘様水庖症:副腎皮質ステロイドとVP16間歌療法による治療例 (2010)
  • Author(s): 平井陽至
  • Organizer: 第20回EBウイルス研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-06
• [Presentation] 蚊刺過敏症における館ウイルス感染-NK細胞クローンの解析 (2009)
  • Author(s): 藤井一恭
  • Organizer: 第24回中国四国ウイルス研究会
  • Place of Presentation: 岡山
  • Year and Date: 2009-07-05
• [Presentation] 皮膚T細胞リンパ腫における新しい治療戦略HDAC阻害薬. (2009)
  • Author(s): 藤井一恭
  • Organizer: 第25回日本皮膚悪性腫瘍学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2009-05-23
• [Presentation] 皮膚T細胞リンパ腫における新しい治療戦略HDAC阻害薬 (2009)
  • Author(s): 藤井一恭
  • Organizer: 第25回日本皮膚悪性腫瘍学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2009-05-23
• [Presentation] Valproic acid, an HDAC inhibitor induces G1-cell cycle arrest in natural killer (NK) cell lines established from patients with chronic active EB virus infection. (2009)
  • Author(s): Fujii K
  • Organizer: ISCL annual meeting, 2009.
  • Place of Presentation: San Francisco, CA
  • Year and Date: 2009-03-05
• [Presentation] Valproic acid, an HDAC inhibitor induces G 1-cell cycle arrest in natural killer (NK) cell lines established from patients with chronic active EB virus infection (2009)
  • Author(s): Fujii Kazuyasu
  • Organizer: ISCL annual meeting, 2009
  • Place of Presentation: San Francisco, CA
  • Year and Date: 2009-03-05
• [Presentation] Valproic acid, an HDAC inhibitor, diminishes lymphoproliferation of EB-virus infected NK cells via G1 cell cycle arrest. (2009)
  • Author(s): K Fujii
  • Organizer: The 34th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Fukuoka
• [Presentation] EBウイルス関連リンパ球増殖症におけるT/NK細胞の組織選択的浸潤と皮膚病変についての解析 (2009)
  • Author(s): 平井陽至
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡
• [Presentation] MTX/インフリキシマブ投与中に生じ、中止により消退したdiffuse large B-cell lymphoma (2009)
  • Author(s): 加持達弥
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡
• [Presentation] 骨髄異形成症候群関連膿皮症と難治性慢性膿皮症は、造血シグナル上昇に関連して発症する (2009)
  • Author(s): 濱田利久
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡
• [Presentation] Production of proinflammatory cytokines without invocation of cytotoxic effects by an Epstein-Barr virus infected NK cell line established from a patient with hypersensitivity to mosquito bites (2009)
  • Author(s): D Suzuki
  • Organizer: 39th annual ESDR meeting
  • Place of Presentation: ブダペスト(ハンガリー)
• [Presentation] Distinct infiltrating cell types in the skin lessions associated EBV-associated T/NK/lymphoproliferative disorders (2009)
  • Author(s): K Fujii
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Low cytotoxicity of EB virus (EBV)-infected NK cell line established from a patient with chronic active EBV infection (2009)
  • Author(s): D Suzuki
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Valproic acid, an HDAC inhibitor, diminishes lymphoproliferation of EB-virus infected NK cells via G1 cell cycle arrest (2009)
  • Author(s): K Fujii
  • Organizer: The 34th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 福岡
• [Presentation] Simultaneous detection of herpes simplex and varicella-zoster viral transcripts in the same skin lesion (2009)
  • Author(s): Y Hirai
  • Organizer: The 34th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 福岡
• [Presentation] Anti-proliferative effect of valproic acid against natural killer-cell lymphoma cell lines. (2008)
  • Author(s): 藤井一恭
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Possibilities of valproic acid as a therapeutic tool for natural killer-cell neoplasms. (2008)
  • Author(s): Fujii K
  • Organizer: Lymphoma Expert meeting
  • Place of Presentation: Kyoto
  • Year and Date: 2008-05-15
• [Presentation] Possibilities of valproic acid as a therapeutic tool for natural killer-cell neoplasms (2008)
  • Author(s): Fujii Kazuyasu
  • Organizer: Lymphoma Expert meeting
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-05-15
• [Presentation] Possibilities of valproic acid as a therapeutic tool for natural killer-cell neoplasms. (2008)
  • Author(s): Fujii K
  • Organizer: International Investigative Dermatology 2008.
  • Place of Presentation: Kyoto
• [Presentation] Possibilities of valproic acid as a therapeutic tool for natural killer-cell neoplasms (2008)
  • Author(s): Fujii Kazuyasu
  • Organizer: International Investigative Dermatology 2008
  • Place of Presentation: Kyoto, Japan
• [Presentation] Anti-proliferative effect of valproic acid against natural killer-cell lymphoma cell lines (2008)
  • Author(s): 藤井一恭
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋