| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Human β-defensin (HBD) -3 has potent and salt-resistant antimicrobial activity against both gram-negativeand gram positive bacteria and this activity, while other HBDs possess antimicrobial activity against only gram-negative strains and it is attenuated under salt existence. We hypothesized that these characteristics are due to two arginine residues in the COOH-terminal of HBD-3 which is not involved in other HBD-3. To investigate, we designated two recombinant mutants of HBD-3; desR HBD-3 in which these two arginine residues were deleted from HBD-3 and NRR-HBD-3 in which these two arginine residues were shifted from COOH-terminal to NH2-terminal and esxamined their antimicrobial activities and salt-resistance. The results showed that these two argine residues are required to exist in the COOH-terminal in order to maintain the activity and salt-resistance of HBD-3.
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