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The role of Wnt signaling pathway in hair regeneration by using mouse adult hair follicle apithelial stem cells

Research Project

Project/Area Number 20790812
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionNara Medical University

Principal Investigator

OUJI Yukiteru  Nara Medical University, 医学部, 助教 (50343421)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords皮膚炎症・再生学 / 毛包再生医学 / Wnt / 再生医学 / 毛包 / 幹細胞 / 皮膚 / b-catenin / 細胞
Research Abstract

Characterization and elucidation of the differentiation mechanism of stem cell in skin hair follicle (hair follicle epithelial stem cells : HFESCs) is important for understanding the differentiation of epithelial cells and hair follicle morphogenesis. Recently, it is thought that the Wnt signaling pathway is critical for regulating a number of basic cell functions, such as cell proliferation, differentiation, and migration, leading to morphogenesis and organogenesis, and we have already reported that the Wnt signal is important for hair reconstitution and hair growth in vitro and in vivo. In this study, we performed cell sorting of mouse HFESCs and examined the effects of the various Wnts (Wnt-3a, -5a, -10b, and -11) on HFESCs in vitro. Interestingly, only HFESCs cultured with Wnt-3a showed proliferated, and expressed stem cell markers compared with control condition. Furthermore, involvement of the canonical Wnt signal pathway was demonstrated by a TCF reporter (pTOPFLASH) assay. Our results suggest that Wnt-3a promotes the proliferation of HFESCs and may play an important role in HFESCs maintenance.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (12 results)

All 2010 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results)

  • [Journal Article] Noriko Ouji-Sageshima, Atsushi Kubo and Shigeaki Ishizaka2010

    • Author(s)
      Yukiteru Ouji, Masahide Yoshikawa, Mariko Nishiofuku
    • Journal Title

      Effects of Wnt-10b on proliferation and differentiation of adult murineskin-derived CD34 and CD49f double-positive cells J Biosci Bioeng (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Wnt-10b on proliferation and differentiation of adult murine skin-derived CD34 and CD49f double-positive cells2010

    • Author(s)
      Ouji Y, Yoshikawa M, Nishiofuku M, Ouji-Sageshima N, Kubo A, Ishizaka S.
    • Journal Title

      J.Biosc.Bioeng. (In press)

    • NAID

      110007700572

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 石坂重昭発毛の再生医学Journal of Nara Medical Association2008

    • Author(s)
      王寺幸輝, 吉川正英
    • Journal Title

      59巻

      Pages: 33-41

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 発毛の再生医学2008

    • Author(s)
      王寺幸輝、吉川正英、石坂重昭
    • Journal Title

      奈良医学雑誌 59

      Pages: 33-41

    • NAID

      120004972940

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 毛包幹細胞を用いた発毛再生-Wntsの影響について-2010

    • Author(s)
      王寺幸輝、吉川正英、石坂重昭
    • Organizer
      日本再生医療学会
    • Place of Presentation
      広島
    • Year and Date
      2010-03-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 石坂重昭毛包幹細胞を用いた発毛再生-Wntsの影響について-2010

    • Author(s)
      王寺幸輝、吉川正英
    • Organizer
      第9回日本再生医療学会総会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Shigeaki Ishizaka Effects of Wnt families in the hair follicle stem cells2009

    • Author(s)
      Yukiteru Ouji, Masahide Yoshikawa
    • Organizer
      第39回日本免疫学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2009-12-03
    • Related Report
      2009 Final Research Report
  • [Presentation] Effects of Wnts as immune regulators on hair follicle stem cells (HFSCs)2009

    • Author(s)
      Ouji Y, Yoshikawa M, Ishizaka S.
    • Organizer
      日本免疫学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2009-12-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 石坂重昭毛包幹細胞の樹立を目指して-毛包幹細胞に対するWnt familyの影響について-2009

    • Author(s)
      王寺幸輝、久保篤史、後藤達志、吉川正英
    • Organizer
      第8回日本再生医療学会総会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-06
    • Related Report
      2009 Final Research Report
  • [Presentation] 毛包幹細胞の樹立を目指して-毛包幹細胞に対するWnt familyの影響について-2009

    • Author(s)
      王寺幸輝、久保篤史、吉川正英、後藤達志、石坂重昭
    • Organizer
      日本再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス皮膚上皮細胞および培養毛包器官におけるWntの影響2008

    • Author(s)
      王寺幸輝、吉川正英、後藤達志、石坂重昭
    • Organizer
      日本免疫学会
    • Place of Presentation
      京都
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Shigeaki Ishizaka Effects of Wnts on differentiation and hair shaft growth in mouse skin epithelial cells and hair follicle organ2008

    • Author(s)
      Yukiteru Ouji, Masahide Yoshikawa, Tatsushi Coto
    • Organizer
      第38回日本免疫学会総会・学術集会
    • Place of Presentation
      京都
    • Year and Date
      2008-12-02
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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