| Project/Area Number |
20790816
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Shimane University |
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Principal Investigator |
DEKIO Itaru Shimane University, 医学部, 講師 (80338128)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 皮膚感染症 / 微生物ゲノム / 皮膚科学 |
|---|
| Research Abstract |
In order to investigate comprehensive profiles of microbiota including unculturable microorganisms in various skin diseases, we examined sampling method from the skin lesions, culture conditions to grow microorganisms from the samples, and extraction method of microbial genomes from the samples. Swab-wash method was found to be suitable for sampling, and optimal culture conditions for culture analysis were discovered. However, we could not find extraction method for microbial genome of various skin microorganisms. As a secondary accomplishment, measurement of TARC level in stratum corneum in the tape-stripped skin samples is revealed to be useful to evaluate severity of skin lesion in atopic dermatitis patients.
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