Investigation of antipruritic effects in atopic dermatitis using nerve repulsion factor Sema3A

• Project/Area Number: 20790818
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Juntendo University
• Principal Investigator: TOMINAGA Mitsutoshi Juntendo University, 大学院・医学研究科, 博士研究員 (50468592)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 痒み / アトピー性皮膚炎 / Semaphorin 3A / 知覚神経 / Sema3A / 難治性かゆみ / 知覚神経線維 / 外用剤 / 難治性掻痒

Research Abstract

This study was performed to investigate antipruritic effects of semaphorin 3A (Sema3A), which is one of nerve repulsion factors, in atopic dermatitis (AD) using NC/Nga mouse, an AD model. Topical application of recombinant human Sema3A ointment on atopic NC/Nga mice significantly suppressed the scratching behavior and dermatitis compared with those of control mice. Therefore, this finding suggested the therapeutic efficacy of Sema3A ointment in pruritus and dermatitis of AD.

Research Products

• [Journal Article] Neurotropin inhibits both capsaicin-induced substance P release and nerve growth factor-induced neurite outgrowth in cultured rat dorsal root ganglion neurons (2010)
  • Author(s): Taneda K, Tominaga M, Tengara S, Ogawa H, Takamori K.
  • Journal Title: Clin Exp Dermatol. 35 Pages: 73-77
  • Peer Reviewed
• [Journal Article] Histological characterization of cutaneous nerve fibers containing gastrin-releasing peptide in atopic dermatitis (2009)
  • Author(s): Tominaga M, Ogawa H, Takamori K.
  • Journal Title: J Invest Dermatol. 129 Pages: 2901-2905
  • Peer Reviewed
• [Journal Article] In vitro model for penetration of sensory nerve fibers on a Matrigel basement membrane: Implications for possible application to intractable pruritus (2009)
  • Author(s): Tominaga M, Kamo A, Tengara S, Ogawa H, Takamori K.
  • Journal Title: Br J Dermatol. 161 Pages: 1028-1037
  • Peer Reviewed
• [Journal Article] Psoralen-ultraviolet A therapy alters epidermal Sema3A and NGF levels and modulates epidermal innervation in atopic dermatitis (2009)
  • Author(s): Tominaga M, Tengara S, Kamo A, Ogawa H, Takamori K.
  • Journal Title: J Dermatol Sci 55 Pages: 40-46
  • NAID: 10025334220
  • Peer Reviewed
• [Journal Article] The penetartion mechanisms of nerve fibers into the epidermis of atopic dermatitis (2009)
  • Author(s): Tominaga M, Takamori K.
  • Journal Title: J Environ Dermatol Cutan Allergol Vol.3No.2 Pages: 70-77
• [Journal Article] オピオイドと痒み (2009)
  • Author(s): 冨永光俊
  • Journal Title: 日本皮膚科学会雑誌 119(13) Pages: 2502-2505
  • NAID: 10028277614
• [Journal Article] 表皮内神経と痒み (2009)
  • Author(s): 冨永光俊、種田研一、高森建二
  • Journal Title: アレルギーの臨床 Vol.29,No.10 Pages: 27-32
• [Journal Article] In vitro model for penetration of sensory nerve fibers on a Matrigel basement membrane : Implications for possible application to intractable pruritus. (2009)
  • Author(s): Tominaga M, Kamo A, Tengara S, Ogawa H, Takamori K
  • Journal Title: British Journal of Dermatology 161 Pages: 1028-1037
  • Peer Reviewed
• [Journal Article] Histological characterization of cutaneous nerve fibers containing gastrin-releasing peptide in NC/Nga mice : an atopic dermatitis model. (2009)
  • Author(s): Tominaga M, Ogawa H, Takamori K
  • Journal Title: Journal of Investigative Dermatology 129 Pages: 2901-2905
  • Peer Reviewed
• [Journal Article] Psoralen-ultraviolet A therapy alters epidermal Sema3A and NGF levels and modulates epidermal innervation in atopic dermatitis. (2009)
  • Author(s): Tominaga M, Tengara S, Kamo A, Ogawa H, Takamori K
  • Journal Title: Journal of Dermatological Science 55 Pages: 40-46
  • NAID: 10025334220
  • Peer Reviewed
• [Journal Article] Decreased production of semaphorin 3A in the lesional skin of atopic dermatitis (2008)
  • Author(s): Tominaga M, Ogawa H, Takamori K.
  • Journal Title: Br J Dermatol 158 Pages: 842-844
  • Peer Reviewed
• [Journal Article] アトピー性皮膚炎のバリア機能異常と痒みの関係 (2008)
  • Author(s): 高森建二、種田研一、冨永光俊
  • Journal Title: アレルギーの臨床 28 Pages: 28-33
• [Journal Article] Decreased production of semaphorin 3A in the lesional skin of atopic dermatitis (2008)
  • Author(s): Tominaga M
  • Journal Title: British Journal of Dermatology 158 Pages: 842-844
  • Peer Reviewed
• [Journal Article] アトピー性皮膚炎のバリア機能異常と痒みの関係 (2008)
  • Author(s): 高森建二
  • Journal Title: アレルギーの臨床 28 Pages: 28-33
  • Peer Reviewed
• [Journal Article] Recent advances in pathophysiological mechanisms of itch
  • Author(s): Tominaga M, Takamori K.
  • Journal Title: Expert Rev Dermatol 2010(in press)
  • Peer Reviewed
• [Presentation] アトピー性皮膚炎の痒みのメカニズム-表皮内神経とオピオイドの観点から- (2010)
  • Author(s): 冨永光俊
  • Organizer: 第5回熊本かゆみ研究会学術講演会
  • Place of Presentation: 熊本
• [Presentation] Regulation of itch in atopic dermatitis-Involvement of Sema3A- (2009)
  • Author(s): Takamori K, Tominaga M, Taneda K, Negi O
  • Organizer: 10th International Congress of Dermatology
  • Place of Presentation: Czech
  • Year and Date: 2009-05-21
• [Presentation] Implications for peripheral opioid systems to pruritus (2009)
  • Author(s): Tominaga M, Takamori K.
  • Organizer: 5th International Workshop for the Study of Itch
  • Place of Presentation: Tokyo, Japan
• [Presentation] Epidermal nerve density is modulated by keratinocyte-produced anosmin-1 (2009)
  • Author(s): Tengara S, Tominaga M, Takamori K
  • Organizer: 5th International Workshop for the Study of Itch
  • Place of Presentation: Tokyo, Japan
• [Presentation] Evaluation of epidermal nerve density and opioid receptor levels in psoriatic itch (2009)
  • Author(s): Taneda K, Tominaga M, Negi O, Kamo A, Tengara S, Ogawa H, Takamori K
  • Organizer: 5th International Workshop for the Study of Itch
  • Place of Presentation: Tokyo, Japan
• [Presentation] オピオイドと痒み (2009)
  • Author(s): 冨永光俊
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡
• [Presentation] アトピー性皮膚炎マウスにおけるガストリン放出ペプチド含有神経の組織学的特性化 (2009)
  • Author(s): 冨永光俊、高森建二
  • Organizer: 第19回国際痒みシンポジウム
  • Place of Presentation: 大阪
• [Presentation] In vitro model for penetration of sensory nerve fibers on a Matrigel basement membrane: Implications for possible application to intractable pruritus (2009)
  • Author(s): 冨永光俊、高森建二
  • Organizer: 第34回日本研究皮膚科学会
  • Place of Presentation: 福岡
• [Presentation] 乾癬の痒みに関する研究-痒みを伴う乾癬皮疹における表皮内神経線維の関与- (2009)
  • Author(s): 種田研一、冨永光俊、根木治、加茂敦子、Suhandy Tengara、高森建二
  • Organizer: 第64回日本皮膚科学会山梨地方会
  • Place of Presentation: 山梨
• [Presentation] Implications for peripheral opioid systems to pruritus (2009)
  • Author(s): Tominaga M, Takamori K
  • Organizer: 5^<th> International Workshop for the Study of Itch
  • Place of Presentation: Tokyo
• [Presentation] Mechanism of itch in atopic dermatitis-Involvement of opioid systems- (2008)
  • Author(s): Tominaga M, Takamori K
  • Organizer: 5th Georg Rajka International Symposium on Atopic Dermatitis (5th ISAD)
  • Place of Presentation: Kyoto, Japan
• [Presentation] Involvement of keratinocyte-derived semaphorin 3A in epidermal innervation of atopic dermatitis (2008)
  • Author(s): Tominaga M, Takamori K
  • Organizer: 5th International Investigative Dermatology (IID)
  • Place of Presentation: Kyoto, Japan
• [Presentation] 乾癬の痒みに関する研究-痒みを伴う乾癬皮疹における表皮内神経線維の関与- (2008)
  • Author(s): 種田研一、冨永光俊、根木治、須賀康、高森建二
  • Organizer: 第23回日本乾癬学会学術大会
  • Place of Presentation: 旭川
• [Presentation] 表皮内でのSema3Aの発現の低下と、表皮内神経伸長を認めたアミロイド苔癬 (2008)
  • Author(s): 種田研一、冨永光俊、根木治、須賀康、高森建二
  • Organizer: 第72回日本皮膚科学会東部支部学術大会
  • Place of Presentation: 秋田
• [Presentation] ラット脊髄後根神経節細胞を用いたノイロトロピンの痒み抑制効果の研究 (2008)
  • Author(s): 種田研一、冨永光俊、根木治、スハンディ・テンガラ、須賀康、高森建二
  • Organizer: 地方会
  • Place of Presentation: 東京
• [Presentation] Involvement of keratinocyte-derived semaphorin 3A in epidermal innervation of atopic dermatitis (2008)
  • Author(s): Tominaga M
  • Organizer: 5^<th> International Investigative Dermatology (IID)
  • Place of Presentation: 京都
• [Book] からだと光の辞典 (2010)
  • Author(s): 冨永光俊、高森建二
  • Publisher: 朝倉書店(in press)
• [Remarks]
  • URL: http://www.urayasu-juntendo.gr.jp/sinryou/f_newsr_hihuka_8.htm
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kankyo_igaku/k6.html
• [Remarks]
  • URL: http://www.urayasu-juntendo.r.j/sinryou/f_newsr_hihuka_8.htm
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kankyo_igaku/k6.html
• [Patent(Industrial Property Rights)] アレルギー性皮膚炎の診断方法 (2008)
  • Inventor(s): 冨永光俊, 高森建二
  • Industrial Property Rights Holder: 順天堂
  • Patent Publication Number: 2008-107275
  • Filing Date: 2008-05-08