Development of novel blood RNA diagnostic system for inflammatory skin disease

• Project/Area Number: 20790824
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Hyogo College of Medicine
• Principal Investigator: TSUDA Tatsuya Hyogo College of Medicine, 医学部, 助教 (80434942)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 炎症性皮膚疾患 / 網羅的解析 / 末梢血 / RNAマーカー / 選抜アレイ / 新規RNAマーカー

Research Abstract

We performed DNA microarray analysis of total RNA obtained from peripheral blood of psoriasis vulgaris and atopic dermatitis patients. We focused on CCL23 and S100P relating with clinical symptoms and other blood data, and tried to develop focused array to detect these two genes with low costs and easy operation. However, developed focused array did not have enough sensitivity.

Research Products

• [Journal Article] Tetracyclines modulate protease-activated receptor 2-mediated proinflammatory reactions in epidermal keratinocytes. (2009)
  • Author(s): Ishikawa C, Tsuda T, Konishi H, Nakagawa N, Yamanishi K.
  • Journal Title: Antimicrobial agents and chemotherapy 53巻 Pages: 1760-1765
  • Peer Reviewed
• [Journal Article] Tetracyclines modulate protease-activated receptor 2-mediated proinflammatory reactions in epidermal keratinocytes (2009)
  • Author(s): Ishikawa C
  • Journal Title: Antimicrobial agents and chemotherapy 53 Pages: 1760-1765
  • Peer Reviewed
• [Journal Article] Identification of preferred substrate sequences for transglutaminase 1-development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin. (2008)
  • Author(s): Sugimura Y, Hosono M, Kitamura M, Tsuda T, Yamanishi K, Maki M, Hitomi K.
  • Journal Title: The FEBS Journal 275巻 Pages: 5667-77
  • Peer Reviewed
• [Journal Article] Adult cutaneous alveolar rhabdomyosarcoma on the face diagnosed by the expression of PAX3-FKHR gene fusion transcripts. (2008)
  • Author(s): Nakagawa N, Tsuda T, Yamamoto M, Ito T, Futani H, Yamanishi K.
  • Journal Title: Journal of Dermatology 35巻 Pages: 462-467
  • NAID: 10024297532
  • Peer Reviewed
• [Journal Article] A novel point mutation of keratin 17 (KRT17) in a Japanese family with pachyonychia congenita type 2: an RNA-based genetic analysis using a single hair bulb. (2008)
  • Author(s): Tsuda T, Ishikawa C, Nakagawa N, Konishi H, Tarutani M, Matsuki M, Yamanishi K.
  • Journal Title: British Journal of Dermatology 159巻 Pages: 730-732
  • Peer Reviewed
• [Journal Article] Cutaneous malignant fibrous histiocytoma (undifferentiated pleomorphic sarcoma) arising in a chronic scalp ulcer of a patient with non-bullous congenital ichthyosiform erythroderma. (2008)
  • Author(s): Fujimoto T, Tsuda T, Yamamoto M, Tarutani M, Natsuaki M, Minami S, Ito T, Kozuka T, Yamanishi K.
  • Journal Title: Journal of the European Academy of Dermatology and Venereology 23巻 Pages: 202-203
  • Peer Reviewed
• [Journal Article] Effect of 14-membered-ring macrolides on production of interleukin-8 mediated by protease-activated receptor 2 in human keratinocytes (2008)
  • Author(s): Tatsuya Tsuda
  • Journal Title: Antimicrobial agents and chemotherapy 52 Pages: 1538-1541
  • Peer Reviewed
• [Journal Article] A novel point mutation of keratin 17 (KRT17) in a Japanese family with pachyonychia congenita type 2 : an RNA-based genetic analysis using a single hair bulb (2008)
  • Author(s): Tatsuya Tsuda
  • Journal Title: British journal of dermatology 159 Pages: 730-732
  • Peer Reviewed
• [Journal Article] Adult cutaneous alveolar rhabdomyosarcoma on the face diagnosed by the expression of PAX3-FKHR gene fusion transcripts (2008)
  • Author(s): Noboru Nakagawa
  • Journal Title: Journal of dermatology 35 Pages: 462-467
  • Peer Reviewed
• [Journal Article] Identification of preferred substrate sequences for transglutaminase 1--development of a novel peptide that can efficiently detect cross-linking enzyme activity in the skin (2008)
  • Author(s): Sugimura Y
  • Journal Title: The FEBS journal 275 Pages: 5667-77
  • Peer Reviewed
• [Presentation] 膿胞性乾癬に対する白血球除去療法 (2009)
  • Author(s): 今井康友、古川紗綾佳、津田達也、上田英一郎、山西清文
  • Organizer: 第87回兵庫県皮膚科医会総会・学術集談会
  • Place of Presentation: 生田神社会館、兵庫県
  • Year and Date: 2009-11-28
• [Presentation] 急性腎障害を合併したケラチン10の新規ミスセンス変異をもつ水庖型先天性魚鱗癬様紅皮症 (2009)
  • Author(s): 山本雅章
  • Organizer: 日本皮膚科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2009-04-25
• [Presentation] 急性腎障害を合併したケラチン10の新規ミスセンス変異をもつ水疱型先天性魚鱗癬様紅皮症 (2009)
  • Author(s): 山本雅章、津田達也、小西弘江、山西清文
  • Organizer: 日本皮膚科学会総会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 瀧澤俊広、トランスグルタミナーゼ1へのアルギニン142点突然変異導入マウスの表現型解析 (2008)
  • Author(s): 津田達也、松木正人、小西弘江、山西清文
  • Organizer: 第22回表皮細胞研究会
  • Place of Presentation: ホテル日航東京
  • Year and Date: 2008-12-06
• [Presentation] Psoriasis like hyperplastic and inflammatory lesions produced by epidermis specific, inducible activation of Raf in mice. (2008)
  • Author(s): M Tarutani, Y Imai, T Tsuda, K Nakanishi, K Yamanishi
  • Organizer: International Investigative Dermatology
  • Place of Presentation: 京都国際会議場
• [Presentation] Serum calcium-binding S100A8/A9 proteins in patients with psoriatic arthritis are derived from not only the skin lesions but also circulating monocytes. (2008)
  • Author(s): S Aochi, K Tsuji, M Sakaguchi, N Huh, T Tsuda, K Yamanishi, M komine, K Iwatasuki
  • Organizer: International Investigative Dermatology 2008
  • Place of Presentation: 京都国際会議場
• [Presentation] Serum calcium-binding S100A8/A9 proteins in patients with psoriatic arthritis are derived from not only the skin lesions but also circulating monocytes (2008)
  • Author(s): Aochi S
  • Organizer: International Investigative Dermatology 2008
  • Place of Presentation: 京都国際会館
• [Presentation] Psoriasis like hyperplastic and inflammatory lesions produced by epidermis specific, inducible activation of Raf in mice (2008)
  • Author(s): Tarutani M
  • Organizer: International Investigative Dermatology 2008
  • Place of Presentation: 京都国際会館