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Functional analysis of GRIN family in the central nervous system

Research Project

Project/Area Number 20790860
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionTsurumi University

Principal Investigator

MOTOTANI Yasumasa  Tsurumi University, 歯学部, 助教 (60421830)

Co-Investigator(Renkei-kenkyūsha) OKAMURA Tasashi  国立国際医療研究センター研究所, ヒト型動物開発研究質, 室長 (00333790)
Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords精神薬理学 / 三量体 / Gタンパク質 / 脳神経 / 動物モデル / Gタンパク
Research Abstract

We have studied about the roles of GRIN family, which binds to Gαi/o subunits, in the brain. In this study, we were able to generate both conditional GRIN1 KO mice and simple GRIN3 KO mice. Also, GRIN1 knockdown by lentivirus based RNAi system was successful. GRIN1 knockdown in retinoic acids induced neuronal P19 cells caused the reduction of GABAB2 receptor without the affect of Gαo expression. Furthermore, stimulation of cannabinoid receptor 1 (CB1) agonist could not activate the ERK pathway in these cells. On the other hand, ablation of GRIN3 in mice led to the decrease of both dopamine D1 and D2 receptors. These observations indicate that GRIN family plays the roles for GPCR function, such as stability and turn ov er in the cell membranes.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (4 results)

All 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Dynamic expression patterns of G protein regulated neuroite outgrowth1 (GRIN1) and its colocalization with Gao implicate roles of Gao-GRIN1 signaling pathway in neuronal cell migration, differentiation and wiring2008

    • Author(s)
      Ikuo Masuho, Yasumasa Mototani, Yoshinori Sahara, Junko Asami, Shun Nakamura, Tohru Kozasa, Takayoshi Inoue
    • Journal Title

      Developmental Dynamics 237(9)

      Pages: 2415-29

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic expression patterns of G protein-regulated inducer of neurite outgrowthl (GRIN1) and its colocalization with Gao implicate roles of Gao- GRIN1 signaling pathway in neuronal cell migration, differentiation and wiring2008

    • Author(s)
      Ikuo Masuho Yasumasa Mototani (co-first author)
    • Journal Title

      Developmental Dynamics 237(9)

      Pages: 24152429-24152429

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Identification of cell-types expressing G protein-regulated induced neurite outgrowth 1(GRIN1) in the developing and adult mice2008

    • Author(s)
      本谷安正
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2009 Final Research Report
  • [Presentation] GRIN1-EGFPトランスジェニックマウスを用いたGRIN1発現細胞の同定2008

    • Author(s)
      本谷安正
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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